A dual-round signal amplification strategy for colorimetric/photoacoustic/fluorescence triple read-out detection of prostate specific antigen

A colorimetric/fluorescence/photoacoustic triple read-out detection of prostate specific antigen (PSA) was developed by using a silica coated Au@Ag core–shell nanorod (denoted Au@Ag@SiO2) based enzyme-linked immunosorbent assay (ELISA) system.

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An acid-responsive all-in-one signal amplification strategy for the ultrasensitive prostate-specific antigen detection

New Journal of Chemistry ◽

10.1039/c9nj03510a ◽

2019 ◽

Vol 43 (40) ◽

pp. 15910-15914 ◽

Cited By ~ 1

Author(s):

Yan Wang ◽

Lijuan Chen ◽

Qingxia Wu ◽

Zejun Wen ◽

Yujing Ren ◽

...

Keyword(s):

Prostate Specific Antigen ◽

Signal Amplification ◽

Specific Antigen ◽

Antigen Detection ◽

One Pot ◽

Colorimetric Immunoassay ◽

Amplification Strategy

A one-pot strategy for the preparation of all-in-one signal tags for the colorimetric immunoassay of prostate-specific antigen.

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Signal amplification strategy for sensitive immunoassay of prostate specific antigen (PSA) based on ferrocene incorporated polystyrene spheres

Analytica Chimica Acta ◽

10.1016/j.aca.2013.07.054 ◽

2013 ◽

Vol 793 ◽

pp. 19-25 ◽

Cited By ~ 29

Author(s):

Lu Ding ◽

Jinmao You ◽

Rongmei Kong ◽

Fengli Qu

Keyword(s):

Prostate Specific Antigen ◽

Signal Amplification ◽

Specific Antigen ◽

Polystyrene Spheres ◽

Amplification Strategy

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CRISPR/Cas13a signal amplification linked immunosorbent assay (CLISA)

10.1101/781237 ◽

2019 ◽

Author(s):

Qian Chen ◽

Tian Tian ◽

Erhu Xiong ◽

Po Wang ◽

Xiaoming Zhou

Keyword(s):

Immunosorbent Assay ◽

Signal Amplification ◽

Limit Of Detection ◽

Enzyme Linked Immunosorbent Assay ◽

Inflammatory Factor ◽

Early Diagnosis Of Cancer ◽

Low Concentrations ◽

Conventional Elisa ◽

Amplification Strategy ◽

Endothelial Growth Factor

ABSTRACTThe enzyme-linked immunosorbent assay (ELISA) is a basic technique used in analytical and clinical investigations. However, conventional ELISA is still not sensitive enough to detect ultra-low concentrations of biomarkers for the early diagnosis of cancer, cardiovascular risk, neurological disorders, and infectious diseases. Herein we show a mechanism utilizing the CRISPR/Cas13a-based signal export amplification strategy, which double-amplifies the output signal by T7 RNA polymerase transcription and CRISPR/Cas13a collateral cleavage activity. This process is termed the CRISPR/Cas13a signal amplification linked immunosorbent assay (CLISA). The proposed method was validated by detecting an inflammatory factor, human interleukin-6 (human IL-6), and a tumor marker, human vascular endothelial growth factor (human VEGF), which achieved limit of detection (LOD) values of 45.81 fg/mL (2.29 fM) and 32.27 fg/m (0.81 fM), respectively, demonstrating that CLISA is at least 102-fold more sensitive than conventional ELISA.

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An Fe3O4/PEDOT:PSS nanocomposite as an advanced electroconductive material for the biosensing of the prostate-specific antigen in unprocessed human plasma samples

Analytical Methods ◽

10.1039/c9ay01988j ◽

2019 ◽

Vol 11 (44) ◽

pp. 5661-5672 ◽

Cited By ~ 6

Author(s):

Mina Shaban ◽

Mohammad Hasanzadeh ◽

Elham Solhi

Keyword(s):

Human Plasma ◽

Prostate Specific Antigen ◽

Signal Amplification ◽

Specific Antigen ◽

Plasma Samples ◽

Real Samples ◽

Human Plasma Samples ◽

Sandwich Type ◽

Amplification Strategy

A novel sandwich-type biosensor based on a signal amplification strategy of various nanocomposites was developed to monitor the prostate-specific antigen (PSA) in real samples.

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