Trichromatic-emission and dual-ratio semiconducting polymer dots as fluorescent probe for simultaneous quantification of Cu2+ and pH in vitro and in vivo

2020 ◽  
Vol 56 (61) ◽  
pp. 8647-8650
Author(s):  
Qiang Zhang ◽  
Junyong Sun ◽  
Rongchao Zhang ◽  
Xueli Chen ◽  
Ningning Chen ◽  
...  
Keyword(s):  
Hela Cells ◽  
Simultaneous Detection ◽  
Blue Color ◽  
Semiconducting Polymer ◽  
Simultaneous Quantification ◽  
Polymer Dots ◽  
Multicolor Imaging ◽  
Semiconducting Polymer Dots

Polymer dots emitting in the red, green and blue color regions, have been successfully applied as lysosome-targeting nanoprobes for the simultaneous detection and multicolor imaging of pH and Cu2+ in HeLa cells and zebrafish.

Brightness Enhancement of Near-Infrared Semiconducting Polymer Dots for in Vivo Whole-Body Cell Tracking in Deep Organs

2018 ◽  
Vol 10 (32) ◽  
pp. 26928-26935 ◽  
Author(s):  
Zhe Zhang ◽  
Ye Yuan ◽  
Zhihe Liu ◽  
Haobin Chen ◽  
Dandan Chen ◽  
...  
Keyword(s):  
Cell Tracking ◽  
Near Infrared ◽  
Whole Body ◽  
Semiconducting Polymer ◽  
Body Cell ◽  
Brightness Enhancement ◽  
Polymer Dots ◽  
Semiconducting Polymer Dots

Tuning the Emission of Semiconducting Polymer Dots from Green to Near-Infrared by Alternating Donor Monomers and Their Applications for in Vivo Biological Imaging

2015 ◽  
Vol 5 (1) ◽  
pp. 154-157 ◽  
Author(s):  
Sz-Yu Liou ◽  
Chi-Shiang Ke ◽  
Jhih-Han Chen ◽  
Yun-Wen Luo ◽  
Shih-Yu Kuo ◽  
...  
Keyword(s):  
Near Infrared ◽  
Biological Imaging ◽  
Semiconducting Polymer ◽  
Polymer Dots ◽  
Semiconducting Polymer Dots

Quinoxaline-Based Semiconducting Polymer Dots for in Vivo NIR-II Fluorescence Imaging

2019 ◽  
Vol 52 (15) ◽  
pp. 5735-5740 ◽  
Author(s):  
Ye Liu ◽  
Jinfeng Liu ◽  
Dandan Chen ◽  
Xiaosha Wang ◽  
Zhenbao Liu ◽  
...  
Keyword(s):  
Fluorescence Imaging ◽  
Semiconducting Polymer ◽  
Polymer Dots ◽  
Semiconducting Polymer Dots

Reversible Ratiometric NADH Sensing Using Semiconducting Polymer Dots

2021 ◽  
Author(s):  
Haobin Chen ◽  
Jiangbo Yu ◽  
Xiaoxiao Men ◽  
Jicheng Zhang ◽  
Zhaoyang Ding ◽  
...  
Keyword(s):  
Semiconducting Polymer ◽  
Polymer Dots ◽  
Semiconducting Polymer Dots

scholarly journals Light-induced crosslinkable semiconducting polymer dots

2015 ◽  
Vol 6 (3) ◽  
pp. 2102-2109 ◽  
Author(s):  
Yue Zhang ◽  
Fangmao Ye ◽  
Wei Sun ◽  
Jiangbo Yu ◽  
I-Che Wu ◽  
...  
Keyword(s):  
Small Molecules ◽  
Chemical Stability ◽  
Semiconducting Polymer ◽  
Polymer Dots ◽  
Semiconducting Polymer Dots ◽  
Physical And Chemical

This paper describes photocrosslinkable Pdots with enhanced colloidal, physical, and chemical stability, and excellent encapsulating ability of functional small molecules.

scholarly journals Optically Encoded Semiconducting Polymer Dots with Single-Wavelength Excitation for Barcoding and Tracking of Single Cells

2017 ◽  
Vol 89 (11) ◽  
pp. 6232-6238 ◽  
Author(s):  
Chun-Ting Kuo ◽  
Hong-Shang Peng ◽  
Yu Rong ◽  
Jiangbo Yu ◽  
Wei Sun ◽  
...  
Keyword(s):  
Single Cells ◽  
Semiconducting Polymer ◽  
Polymer Dots ◽  
Semiconducting Polymer Dots

scholarly journals Single-Chain Semiconducting Polymer Dots

Langmuir ◽  
2014 ◽  
Vol 31 (1) ◽  
pp. 499-505 ◽  
Author(s):  
Fangmao Ye ◽  
Wei Sun ◽  
Yue Zhang ◽  
Changfeng Wu ◽  
Xuanjun Zhang ◽  
...  
Keyword(s):  
Single Chain ◽  
Semiconducting Polymer ◽  
Polymer Dots ◽  
Semiconducting Polymer Dots

BAF is required for emerin assembly into the reforming nuclear envelope

2001 ◽  
Vol 114 (24) ◽  
pp. 4575-4585 ◽  
Author(s):  
Tokuko Haraguchi ◽  
Takako Koujin ◽  
Miriam Segura-Totten ◽  
Kenneth K. Lee ◽  
Yosuke Matsuoka ◽  
...  
Keyword(s):  
Nuclear Envelope ◽  
Hela Cells ◽  
Core Region ◽  
Lamin B ◽  
The Core ◽  
Double Stranded Dna ◽  
Recessive Form ◽  
Nuclear Envelope Assembly

Mutations in emerin cause the X-linked recessive form of Emery-Dreifuss muscular dystrophy (EDMD). Emerin localizes at the inner membrane of the nuclear envelope (NE) during interphase, and diffuses into the ER when the NE disassembles during mitosis. We analyzed the recruitment of wildtype and mutant GFP-tagged emerin proteins during nuclear envelope assembly in living HeLa cells. During telophase, emerin accumulates briefly at the ‘core’ region of telophase chromosomes, and later distributes over the entire nuclear rim. Barrier-to-autointegration factor (BAF), a protein that binds nonspecifically to double-stranded DNA in vitro, co-localized with emerin at the ‘core’ region of chromosomes during telophase. An emerin mutant defective for binding to BAF in vitro failed to localize at the ‘core’ in vivo, and subsequently failed to localize at the reformed NE. In HeLa cells that expressed BAF mutant G25E, which did not show ‘core’ localization, the endogenous emerin proteins failed to localize at the ‘core’ region during telophase, and did not assemble into the NE during the subsequent interphase. BAF mutant G25E also dominantly dislocalized LAP2β and lamin A from the NE, but had no effect on the localization of lamin B. We conclude that BAF is required for the assembly of emerin and A-type lamins at the reforming NE during telophase, and may mediate their stability in the subsequent interphase.

scholarly journals Screening of lung cancer biomarker-proteins with a multiplex electrochemical sensor system based on aptamers

2018 ◽  
Vol 17 (3) ◽  
pp. 13-21
Author(s):  
Yu. E. Glazyrin ◽  
A. V. Shabalina ◽  
K. A. Ryginskaya ◽  
S. S. Zamay ◽  
V. A. Kolovski ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Blood Plasma ◽  
Simultaneous Detection ◽  
Selection Procedure ◽  
Cancer Tissue ◽  
Dna Aptamers ◽  
Affinity Enrichment ◽  
In Blood Plasma

The aimof this work is the development and demonstration of the method of simultaneous detection of several biomarkers of lung cancer in the blood plasma of patients using a multiplex electrochemical testing system based on DNA aptamers. DNA aptamers are a new class of synthetic affinity probes obtained by in vitro or in vivo selection procedure by the systematic evolution of ligands by exponential enrichment (SELEX).Materials and methods.A set of aptamers obtained previously by selection for postoperative lung cancer tissue was used to create a multiplex electrochemical biochip. Identification of aptamer target proteins was performed using a modified affinity enrichment method (AptaBID). Molecular targets for the used set of aptamers to lung cancer were defined as vimentin, defensin, a light chain of myosin, tubulin alpha 1-B, neutrophil elastase and A1 elongation factor 1.Measurements of the presence of these biomarker proteins in blood plasma were carried out using electrochemical detection. The difference between peak heights before and after plasma deposition on the electrodes modified by aptamers was considered as a response of the system to the presence of protein onco-markers in blood plasma. Blood plasma of healthy volunteers was used as control.Results. Research showed that in the blood plasma of all the patients with lung cancer the content of biomarker proteins that bind to aptamers on electrode surfaces was increased. The increased content of these proteins in the blood plasma of patients suggests the presence of invasiveness and metastasis of tumors and their chemo-resistance.

Sign in / Sign up

Export Citation Format

Share Document