Oleacein inhibits STAT3, activates the apoptotic machinery, and exerts anti-metastatic effects in the SH-SY5Y human neuroblastoma cells

2020 ◽  
Vol 11 (4) ◽  
pp. 3271-3279 ◽  
Author(s):  
Santa Cirmi ◽  
Marilena Celano ◽  
Giovanni Enrico Lombardo ◽  
Valentina Maggisano ◽  
Antonio Procopio ◽  
...  
Keyword(s):  
Cell Adhesion ◽  
Olive Oil ◽  
Neuroblastoma Cells ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Apoptotic Death ◽  
Cell Adhesion And Migration ◽  
And Migration ◽  
Dependent Pathway

The present findings demonstrate that oleacein, the main secoiridoid of extravirgin olive oil, acts against neuroblastoma cells, by reducing cell adhesion and migration, inducing apoptotic death and modulating STAT3-dependent pathway.

scholarly journals Shielding of the Geomagnetic Field Alters Actin Assembly and Inhibits Cell Motility in Human Neuroblastoma Cells

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Wei-Chuan Mo ◽  
Zi-Jian Zhang ◽  
Dong-Liang Wang ◽  
Ying Liu ◽  
Perry F. Bartlett ◽  
...  
Keyword(s):  
Actin Cytoskeleton ◽  
Geomagnetic Field ◽  
Neuroblastoma Cells ◽  
Actin Assembly ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Cell Adhesion And Migration ◽  
Underlying Mechanisms ◽  
And Migration

Abstract Accumulating evidence has shown that absence of the geomagnetic field (GMF), the so-called hypomagnetic field (HMF) environment, alters the biological functions in seemingly non-magnetosensitive cells and organisms, which indicates that the GMF could be sensed by non-iron-rich and non-photo-sensing cells. The underlying mechanisms of the HMF effects on those cells are closely related to their GMF sensation but remain poorly understood so far. Previously, we found that the HMF represses expressions of genes associated with cell migration and cytoskeleton assembly in human neuroblastoma cells (SH-SY5Y cell line). Here, we measured the HMF-induced changes on cell morphology, adhesion, motility and actin cytoskeleton in SH-SY5Y cells. The HMF inhibited cell adhesion and migration accompanied with a reduction in cellular F-actin amount. Moreover, following exposure to the HMF, the number of cell processes was reduced and cells were smaller in size and more round in shape. Furthermore, disordered kinetics of actin assembly in vitro were observed during exposure to the HMF, as evidenced by the presence of granule and meshed products. These results indicate that elimination of the GMF affects assembly of the motility-related actin cytoskeleton, and suggest that F-actin is a target of HMF exposure and probably a mediator of GMF sensation.

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