scholarly journals Production and characterization of chitooligosaccharides by the fungal chitinase Chit42 immobilized on magnetic nanoparticles and chitosan beads: selectivity, specificity and improved operational utility

RSC Advances ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 5529-5536
Author(s):  
Peter E. Kidibule ◽  
Jessica Costa ◽  
Andrea Atrei ◽  
Francisco J. Plou ◽  
Maria Fernandez-Lobato ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Industrial Application ◽  
Chitosan Beads

Chitin-active enzymes are of great biotechnological interest due to the wide industrial application of chitinolytic materials.

Expression and characterization of an α-glucosidase from Thermoanaerobacter ethanolicus JW200 with potential for industrial application

Biologia ◽  
2009 ◽  
Vol 64 (6) ◽  
Author(s):  
Yue-Hong Wang ◽  
Yu Jiang ◽  
Zuo-Ying Duan ◽  
Wei-Lan Shao ◽  
Hua-Zhong Li
Keyword(s):  
Escherichia Coli ◽  
Heat Shock ◽  
Optimal Condition ◽  
Industrial Application ◽  
Conversion Rate ◽  
Hydrolytic Activity ◽  
Thermoanaerobacter Ethanolicus ◽  
Transglycosylation Activity

AbstractIn this study, a new α-glucosidase gene from Thermoanaerobacter ethanolicus JW200 was cloned and expressed in Escherichia coli by a novel heat-shock vector pHsh. The recombinant α-glucosidase exhibited its maximum hydrolytic activity at 70°C and pH 5.0∼5.5. With p-nitrophenyl-α-D-glucoside as a substrate and under the optimal condition (70°C, pH 5.5), K m and V max of the enzyme was 1.72 mM and 39 U/mg, respectively. The purified α-glucosidase could hydrolyze oligosaccharides with both α-1,4 and α-1,6 linkages. The enzyme also had strong transglycosylation activity when maltose was used as sugar donor. The transglucosylation products towards maltose are isomaltose, maltotriose, panose, isomaltotriose and tetrasaccharides. The enzyme could convert 400 g/L maltose to oligosaccharides with a conversion rate of 52%, and 83% of the oligosaccharides formed were prebiotic isomaltooligosaccharides (containing isomaltose, panose and isomaltotriose).

scholarly journals Adsorption Studies on Magnetic Nanoparticles Functionalized with Silver to Remove Nitrates from Waters

Water ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 1757
Author(s):  
Yesica Vicente-Martínez ◽  
Manuel Caravaca ◽  
Antonio Soto-Meca ◽  
Miguel Ángel Martín-Pereira ◽  
María del Carmen García-Onsurbe
Keyword(s):  
Magnetic Nanoparticles ◽  
Nitrate Content ◽  
Adsorption Efficiency ◽  
Experimental Conditions ◽  
Dependent Behavior ◽  
Before And After ◽  
Naoh Solution ◽  
High Concentrations ◽  
Interference Studies

This paper presents a novel procedure for the treatment of contaminated water with high concentrations of nitrates, which are considered as one of the main causes of the eutrophication phenomena. For this purpose, magnetic nanoparticles functionalized with silver (Fe3O4@AgNPs) were synthesized and used as an adsorbent of nitrates. Experimental conditions, including the pH, adsorbent and adsorbate dose, temperature and contact time, were analyzed to obtain the highest adsorption efficiency for different concentration of nitrates in water. A maximum removal efficiency of 100% was reached for 2, 5, 10 and 50 mg/L of nitrate at pH = 5, room temperature, and 50, 100, 250 and 500 µL of Fe3O4@AgNPs, respectively. The characterization of the adsorbent, before and after adsorption, was performed by energy dispersive X-ray spectroscopy, scanning electron microscopy, Brunauer-Emmett-Teller analysis and Fourier-transform infrared spectroscopy. Nitrates can be desorbed, and the adsorbent can be reused using 500 µL of NaOH solution 0.01 M, remaining unchanged for the first three cycles, and exhibiting 90% adsorption efficiency after three regenerations. A deep study on equilibrium isotherms reveals a pH-dependent behavior, characterized by Langmuir and Freundlich models at pH = 5 and pH = 1, respectively. Thermodynamic studies were consistent with physicochemical adsorption for all experiments but showed a change from endothermic to exothermic behavior as the temperature increases. Interference studies of other ions commonly present in water were carried out, enabling this procedure as very selective for nitrate ions. In addition, the method was applied to real samples of seawater, showing its ability to eliminate the total nitrate content in eutrophized waters.

Curcuminoids-conjugatiked multicore magnetic nanoparticles: design and characterization of a potential theranostic nanoplatform

2021 ◽  
pp. 160448
Author(s):  
Evelyn C.S. Santos ◽  
Jamili A. Cunha ◽  
Marcel G. Martins ◽  
Bianca M. Galeano-Villar ◽  
Richard J. Caraballo-Vivas ◽  
...  
Keyword(s):  
Magnetic Nanoparticles

Characterization of as-synthesized FeCo magnetic nanoparticles by coprecipitation method

2013 ◽  
Vol 113 (17) ◽  
pp. 17A313 ◽  
Author(s):  
Jinu Kim ◽  
Jinbae Kim ◽  
Jongryoul Kim ◽  
Ki Hyeon Kim
Keyword(s):  
Magnetic Nanoparticles ◽  
Coprecipitation Method

scholarly journals Low temperature solventless synthesis and characterization of Ni and Fe magnetic nanoparticles

2012 ◽  
Vol 48 (66) ◽  
pp. 8237 ◽  
Author(s):  
Liis Seinberg ◽  
Shinpei Yamamoto ◽  
Ruwan Gallage ◽  
Masahiko Tsujimoto ◽  
Yoji Kobayashi ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Low Temperature ◽  
Synthesis And Characterization ◽  
Solventless Synthesis

Semi-wet growth and characterization of multi-functional nano-engineered mixed metal oxides for industrial application

2021 ◽  
Vol 67 (4) ◽  
pp. 100542
Author(s):  
Laxman Singh ◽  
Ravikant Sharma ◽  
Narayan Singh ◽  
Atendra Kumar ◽  
Dev K Mahato ◽  
...  
Keyword(s):  
Metal Oxides ◽  
Industrial Application ◽  
Mixed Metal Oxides ◽  
Mixed Metal

Synthesis and characterization of PEG coated hollow Fe3O4 magnetic nanoparticles as a drug carrier

2021 ◽  
pp. 131357
Author(s):  
Xianhong Da ◽  
Ruonan Li ◽  
Xiang Li ◽  
Yunshu Lu ◽  
Fenfen Gu ◽  
...  
Keyword(s):  
Magnetic Nanoparticles ◽  
Drug Carrier ◽  
Synthesis And Characterization ◽  
Fe3o4 Magnetic Nanoparticles
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