A bone regeneration strategy via dual delivery of demineralized bone matrix powder and hypoxia-pretreated bone marrow stromal cells using an injectable self-healing hydrogel

2021 ◽  
Author(s):  
Donghai Li ◽  
Zhouyuan Yang ◽  
Xin Zhao ◽  
Yue Luo ◽  
Yi Ou ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Regeneration ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Marrow Stromal Cells ◽  
Self Healing ◽  
Matrix Powder ◽  
Demineralized Bone

Dual delivery of demineralized bone matrix (DBM) powder and hypoxia-pretreated bone marrow stromal cells (BMSCs) using an injectable self-healing hydrogel enhances bone regeneration.

Transplanted Endothelial Cells Enhance Orthotopic Bone Regeneration

2006 ◽  
Vol 85 (7) ◽  
pp. 633-637 ◽  
Author(s):  
D. Kaigler ◽  
P.H. Krebsbach ◽  
Z. Wang ◽  
E.R. West ◽  
K. Horger ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Endothelial Cells ◽  
Bone Formation ◽  
Bone Regeneration ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Bone Matrix ◽  
Marrow Stromal Cells ◽  
Calvarial Defects ◽  
Osseous Defect

The aim of this study was to determine if endothelial cells could enhance bone marrow stromal-cell-mediated bone regeneration in an osseous defect. Using poly-lactide-co-glycolide scaffolds as cell carriers, we transplanted bone marrow stromal cells alone or with endothelial cells into 8.5-mm calvarial defects created in nude rats. Histological analyses of blood vessel and bone formation were performed, and microcomputed tomography (μCT) was used to assess mineralized bone matrix. Though the magnitude of the angiogenic response between groups was the same, μCT analysis revealed earlier mineralization of bone in the co-transplantation condition. Ultimately, there was a significant increase (40%) in bone formation in the co-transplantation group (33 ± 2%), compared with the transplantation of bone marrow stromal cells alone (23 ± 3%). Analysis of these data demonstrates that, in an orthotopic site, transplanted endothelial cells can influence the bone-regenerative capacity of bone marrow stromal cells.

Design of hydroxyapatite bioceramics with micro-/nano-topographies to regulate the osteogenic activities of bone morphogenetic protein-2 and bone marrow stromal cells

Nanoscale ◽  
2020 ◽  
Vol 12 (13) ◽  
pp. 7284-7300 ◽  
Author(s):  
Xiangfeng Li ◽  
Minjun Liu ◽  
Fuying Chen ◽  
Yuyi Wang ◽  
Menglu Wang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Regeneration ◽  
Bone Morphogenetic Protein ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Bone Morphogenetic Protein 2 ◽  
Natural Bone ◽  
Morphogenetic Protein ◽  
Bone Apatite

Biomimicking the nanostructure of natural bone apatite to enhance the bioactivity of hydroxyapatite (HA) biomaterials is an eternal topic in the bone regeneration field.

Short-Time Survival and Engraftment of Bone Marrow Stromal Cells in an Ectopic Model of Bone Regeneration

2010 ◽  
Vol 16 (2) ◽  
pp. 489-499 ◽  
Author(s):  
Paolo Giannoni ◽  
Silvia Scaglione ◽  
Antonio Daga ◽  
Cristina Ilengo ◽  
Michele Cilli ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Regeneration ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Short Time

Bone marrow stromal cells with a combined expression of BMP-2 and VEGF-165 enhanced bone regeneration

2011 ◽  
Vol 6 (1) ◽  
pp. 015013 ◽  
Author(s):  
Caiwen Xiao ◽  
Huifang Zhou ◽  
Guangpeng Liu ◽  
Peng Zhang ◽  
Yao Fu ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Regeneration ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells

Demineralized bone matrix-based microcarrier scaffold favors vascularized large bone regeneration in vivo in a rat model

2018 ◽  
Vol 33 (2) ◽  
pp. 182-195 ◽  
Author(s):  
Qiannan Li ◽  
Wenjie Zhang ◽  
Guangdong Zhou ◽  
Yilin Cao ◽  
Wei Liu ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Stem Cells ◽  
Bone Marrow ◽  
Bone Regeneration ◽  
Demineralized Bone Matrix ◽  
Bone Matrix ◽  
Matrix Group ◽  
Micro Computed Tomography ◽  
Demineralized Bone

Insufficient neo-vascularization of in vivo implanted cell-seeded scaffold remains a major bottleneck for clinical translation of engineered bone formation. Demineralized bone matrix is an ideal bone scaffold for bone engineering due to its structural and biochemical components similar to those of native bone. We hypothesized that the microcarrier form of demineralized bone matrix favors ingrowth of vessels and bone regeneration upon in vivo implantation. In this study, a rat model of femoral vessel pedicle-based bone engineering was employed by filling the demineralized bone matrix scaffolds inside a silicone chamber that surrounded the vessel pedicles, and to compare the efficiency of vascularized bone regeneration between microcarrier demineralized bone matrix and block demineralized bone matrix. The results showed that bone marrow stem cells better adhered to microcarrier demineralized bone matrix and produced more extracellular matrices during in vitro culture. After in vivo implantation, microcarrier demineralized bone matrix seeded with bone marrow stem cells formed relatively more bone tissue than block demineralized bone matrix counterpart at three months upon histological examination. Furthermore, micro-computed tomography three-dimensional reconstruction showed that microcarrier demineralized bone matrix group regenerate significantly better and more bone tissues than block demineralized bone matrix both qualitatively and quantitatively (p < 0.05). Moreover, micro-computed tomography reconstructed angiographic images also demonstrated significantly enhanced tissue vascularization in microcarrier demineralized bone matrix group than in block demineralized bone matrix group both qualitatively and quantitatively (p < 0.05). Anti-CD31 immunohistochemical staining of (micro-) vessels and semi-quantitative analysis also evidenced enhanced vascularization of regenerated bone in microcarrier demineralized bone matrix group than in block demineralized bone matrix group (p < 0.05). In conclusion, the microcarrier form of demineralized bone matrix is an ideal bone regenerative scaffold due to its advantages of osteoinductivity and vascular induction, two essentials for in vivo bone regeneration.

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