Genetic analysis with pyrosequencing using loop pipetting and a light dependent resistor

Analytical Methods ◽

10.1039/d1ay01123e ◽

2021 ◽

Author(s):

Faisal Iqbal ◽

Muhammad Imran Shabbir

Keyword(s):

Genetic Analysis ◽

Point Of Care ◽

Light Sensor ◽

Dna Sequencer

A DNA sequencer called LoopSeeq was developed using pyrosequencing chemistry where iterative addition of dNTPs was performed by contact dispensing through a loop pipette and detection of chemiluminescence with inexpensive light sensor LDR for point-of-care applications.

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Genetic Analysis of H1N1 Influenza Virus from Throat Swab Samples in a Microfluidic System for Point-of-Care Diagnostics

Journal of the American Chemical Society ◽

10.1021/ja203981w ◽

2011 ◽

Vol 133 (23) ◽

pp. 9129-9135 ◽

Cited By ~ 146

Author(s):

B. Scott Ferguson ◽

Steven F. Buchsbaum ◽

Ting-Ting Wu ◽

Kuangwen Hsieh ◽

Yi Xiao ◽

...

Keyword(s):

Influenza Virus ◽

Genetic Analysis ◽

Throat Swab ◽

Point Of Care ◽

H1n1 Influenza ◽

Microfluidic System ◽

H1n1 Influenza Virus ◽

Point Of Care Diagnostics

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Label-Free Colorimetric Detection of Urine Glucose Based on Color Fading Using Smartphone Ambient-Light Sensor

Chemosensors ◽

10.3390/chemosensors8010010 ◽

2020 ◽

Vol 8 (1) ◽

pp. 10 ◽

Cited By ~ 1

Author(s):

Wang ◽

Guo ◽

Hu ◽

Liang ◽

Li ◽

...

Keyword(s):

Limit Of Detection ◽

Colorimetric Assay ◽

Point Of Care ◽

Colorimetric Detection ◽

Diabetic Patients ◽

Ambient Light ◽

Label Free ◽

Urine Glucose ◽

Light Sensor

In this work, a label-free colorimetric assay was developed for the determination of urine glucose using smartphone ambient-light sensor (ALS). Using horseradish peroxidase—hydrogen peroxide—3,3′,5,5′-tetramethylbenzidine (HRP-H2O2-TMB) colored system, quantitative H2O2 was added to samples to-be-determined for deepest color. The presence of glucose oxidase in urine led to the formation of H2O2 and the reduction of TMBred. As a result of this, the color of the urine faded and the solution changed from deep blue to light blue. We measured the illuminance of the transmitted light by a smartphone ambient light sensor, and thereby color changes were used to calculate the content of urine glucose. After method validation, this colorimetric assay was practically applied for the determination of urine samples from diabetic patients. Good linearity was obtained in the range of 0.039–10.000 mg/mL (R2 = 0.998), and a limit of detection was 0.005 mg/mL. Our method was had high accuracy, sensitivity, simplicity, rapidity, and visualization, providing a new sensor to be potentially applicable for point-of-care detection of urine glucose.

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Point of care sensing and biosensing using ambient light sensor of smartphone: Critical review

TrAC Trends in Analytical Chemistry ◽

10.1016/j.trac.2018.11.014 ◽

2019 ◽

Vol 110 ◽

pp. 393-400 ◽

Cited By ~ 30

Author(s):

Sibasish Dutta

Keyword(s):

Critical Review ◽

Point Of Care ◽

Ambient Light ◽

Light Sensor

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A handheld flow genetic analysis system (FGAS): towards rapid, sensitive, quantitative and multiplex molecular diagnosis at the point-of-care level

Lab on a Chip ◽

10.1039/c5lc00139k ◽

2015 ◽

Vol 15 (12) ◽

pp. 2597-2605 ◽

Cited By ~ 30

Author(s):

Bowen Shu ◽

Chunsun Zhang ◽

Da Xing

Keyword(s):

Genetic Analysis ◽

Real Time ◽

Molecular Diagnosis ◽

Point Of Care ◽

Care Level ◽

Microfluidic Platform ◽

Analysis System

An ultra-compact, battery-powered and easy-to-use microfluidic platform is proposed for rapid, sensitive, multiplex and real-time quantification and point-of-care genetic analysis.

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Rapid Determination of Monozygous Twinning with a Microfabricated Capillary Array Electrophoresis Genetic-Analysis Device1

Clinical Chemistry ◽

10.1373/clinchem.2007.102319 ◽

2008 ◽

Vol 54 (6) ◽

pp. 1080-1084 ◽

Cited By ~ 7

Author(s):

Stephanie H I Yeung ◽

Igor L Medintz ◽

Susan A Greenspoon ◽

Richard A Mathies

Keyword(s):

Genetic Analysis ◽

Rapid Determination ◽

Point Of Care ◽

Pcr Amplification ◽

Control Individual ◽

Unrelated Control ◽

Capillary Array Electrophoresis ◽

Genotypic Analysis ◽

Diagnostic Technology ◽

Capillary Array

Abstract Background: Microfabricated genetic-analysis devices have great potential for delivering complex clinical diagnostic technology to the point of care. As a demonstration of the potential of these devices, we used a microfabricated capillary array electrophoresis (μCAE) instrument to rapidly characterize the familial and genotypic relationship of twins who had been assigned fraternal (dizygous) status at birth. Methods: We extracted the genomic DNA from buccal samples collected from the twin sons, the parents, another sibling, and an unrelated control individual. We then carried out multiplex PCR amplification of sequences at 16 short tandem repeat loci commonly used in forensic identity testing. We simultaneously separated the amplicons from all of the individuals on a μCAE device and fluorescently detected the amplicons with single-base resolution in <30 min. Results: The genotypic analysis confirmed the identical status of the twins and revealed, in conjunction with the medical data, that their twin status arose from the rarer dichorionic, diamniotic process. Conclusions: The ability to rapidly analyze complex genetic samples with μCAE devices demonstrates that this approach can help meet the growing need for rapid genetics-based diagnostics.

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