A Ligation-driven CRISPR-Cas Biosensing Platform for Non-Nucleic Acid Target Detections

2021 ◽  
Author(s):  
Jiali Zhao ◽  
Zhen Tan ◽  
Liu Wang ◽  
Chunyang Lei ◽  
Zhou Nie

Herein, we describe a CRISPR-Cas12a sensing platform activated by DNA ligation reaction for the sensitive detection of non-nucleic acid targets, including NAD+, ATP and polynucleotide kinase (PNK). In this design,...

Nanoscale ◽  
2014 ◽  
Vol 6 (12) ◽  
pp. 6866-6872 ◽  
Author(s):  
Jia Ge ◽  
Li-Juan Tang ◽  
Qiang Xi ◽  
Xi-Ping Li ◽  
Ru-Qin Yu ◽  
...  

A nanobiosensor was developed based on a WS2 nanosheet for detection of T4 polynucleotide kinase with low interference and superior sensitivity.


RSC Advances ◽  
2016 ◽  
Vol 6 (94) ◽  
pp. 91077-91082 ◽  
Author(s):  
Jia Ge ◽  
Zhen-Zhen Dong ◽  
Lin Zhang ◽  
Qi-Yong Cai ◽  
Dong-Mei Bai ◽  
...  

A novel label-free biosensor for high sensing of NAD+ based on dsDNA-templated CuNPs and DNA ligation reaction.


2018 ◽  
Vol 90 (24) ◽  
pp. 14507-14513 ◽  
Author(s):  
Lili Tong ◽  
Xiuxiu Wang ◽  
Wen Gao ◽  
Zhenhua Liu ◽  
Zhenzhen Chen ◽  
...  

2017 ◽  
Vol 33 (7) ◽  
pp. 783-788 ◽  
Author(s):  
Chao HU ◽  
Xiang Juan KONG ◽  
Ru Qin YU ◽  
Ting Ting CHEN ◽  
Xia CHU

2020 ◽  
Vol 12 (8) ◽  
pp. 1085-1093
Author(s):  
Justin M. Rosenbohm ◽  
James M. Robson ◽  
Rishabh Singh ◽  
Rose Lee ◽  
Jane Y. Zhang ◽  
...  

Rapid electrostatic enrichment of DNA from urine samples for improved isothermal nucleic acid amplification-based detection of Trichomonas vaginalis.


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