scholarly journals Tug-of-war: molecular dynamometers against living cells for analyzing sub-piconewton interaction of specific protein with cell membrane

2021 ◽  
Author(s):  
Huipu Liu ◽  
Yunlong Chen ◽  
Jiawei Wang ◽  
Yuanjiao Yang ◽  
Huangxian Ju
Keyword(s):  
Membrane Proteins ◽  
Cell Membrane ◽  
Specific Protein ◽  
Living Cells ◽  
Membrane Interactions ◽  
Membrane Interaction ◽  
Protein Membrane ◽  
Functional Regulation ◽  
Signal Transductions

Protein-membrane interactions play important roles in signal transductions and functional regulation of membrane proteins. Here, we design a molecular dynamometer (MDM) for analyzing protein-membrane interaction on living cells. The MDM...

Specific protein-membrane interactions promote packaging of metallo-β-lactamases into outer membrane vesicles

2021 ◽  
Author(s):  
Carolina López ◽  
Alessio Prunotto ◽  
Guillermo Bahr ◽  
Robert A. Bonomo ◽  
Lisandro J. González ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Electrostatic Interactions ◽  
Active Form ◽  
Membrane Vesicles ◽  
Specific Protein ◽  
Outer Membrane Vesicles ◽  
Soluble Enzyme ◽  
Membrane Interactions ◽  
Resistance Determinants ◽  
Protein Membrane

Outer membrane vesicles (OMVs) act as carriers of bacterial products such as plasmids and resistance determinants, including metallo-β-lactamases. The lipidated, membrane-anchored metallo-β-lactamase NDM-1 can be detected in Gram-negative OMVs. The soluble domain of NDM-1 also forms electrostatic interactions with the membrane. Herein, we show that these interactions promote its packaging into OMVs produced by Escherichia coli . We report that favorable electrostatic protein-membrane interactions are also at work in the soluble enzyme IMP-1, while being absent in VIM-2. These interactions correlate with an enhanced incorporation of IMP-1 compared to VIM-2 into OMVs. Disruption of these interactions in NDM-1 and IMP-1 impairs their inclusion into vesicles, confirming their role in defining the protein cargo in OMVs. These results also indicate that packaging of metallo-β-lactamases into vesicles in their active form is a common phenomenon that involves cargo selection based on specific molecular interactions.

scholarly journals Estimating the accuracy of the MARTINI model towards the investigation of Peripheral Protein-Membrane Interactions

2020 ◽  
Author(s):  
Sriraksha Srinivasan ◽  
Valeria Zoni ◽  
Stefano Vanni
Keyword(s):  
Membrane Proteins ◽  
Biological Processes ◽  
Cellular Membranes ◽  
Membrane Interactions ◽  
Peripheral Protein ◽  
Peripheral Membrane Proteins ◽  
Protein Membrane

Peripheral membrane proteins play a major role in numerous biological processes by transiently associating with cellular membranes, often with extreme membrane specificity. Because of the short-lived nature of these interactions,...

LATERAL DIFFUSION OF MEMBRANE PROTEINS AT CELL MEMBRANE

2010 ◽  
Vol 24 (14) ◽  
pp. 1533-1540
Author(s):  
LEI FAN ◽  
TIAN YOU FAN
Keyword(s):  
Membrane Proteins ◽  
Cell Membrane ◽  
Fluid Velocity ◽  
Complex Structure ◽  
Fluid Motion ◽  
Lateral Diffusion ◽  
Living Cells ◽  
Dominant Factor ◽  
Protein Size ◽  
And Diffusion

The cell membrane is an important organ of living cells, which has a complex structure influenced by the interaction between membrane proteins and cell membrane. On the basis of fluid motion and diffusion interaction, a simple model is proposed to evaluate quantitatively the effects of the protein size and membrane fluid velocity on the lateral diffusion of membrane proteins at the cell membrane. The study shows that the diffusion coefficient is a dominant factor on the lateral diffusion.

scholarly journals Specific protein-membrane interactions promote the export of metallo-β-lactamases via outer membrane vesicles

2021 ◽  
Author(s):  
Carolina López ◽  
Alessio Prunotto ◽  
Guillermo Bahr ◽  
Robert A. Bonomo ◽  
Lisandro J. González ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Electrostatic Interactions ◽  
Active Form ◽  
Membrane Vesicles ◽  
Specific Protein ◽  
Outer Membrane Vesicles ◽  
Membrane Interactions ◽  
Molecular Features ◽  
Protein Membrane ◽  
Enhanced Secretion

ABSTRACTOuter membrane vesicles (OMVs) act as carriers of resistance determinants such as metallo-β-lactamases. The metallo-β-lactamase NDM-1 is present in OMVs produced by Gram-negative bacteria since it is a lipidated, membrane-anchored protein. The soluble domain of NDM-1 also forms electrostatic interactions with the membrane. Herein, we show that these interactions promote its export into OMVs produced by Escherichia coli. We report that favorable electrostatic protein-membrane interactions are also at work in the soluble enzyme IMP-1, while being absent in VIM-2. These interactions correlate with an enhanced secretion into OMVs of IMP-1 compared to VIM-2. Disruption of these interactions in NDM-1 and IMP-1 impairs export into vesicles, confirming their role in defining the protein cargo in OMVs. These results also indicate that export of metallo-β-lactamases into vesicles in their active form is a common phenomenon that involves cargo selection based on molecular features.

In situ fluorescent profiling of living cell membrane proteins at a single-molecule level

2019 ◽  
Vol 55 (28) ◽  
pp. 4043-4046 ◽  
Author(s):  
Yuanyuan Fan ◽  
Lu Li ◽  
Meng Lu ◽  
Haibin Si ◽  
Bo Tang
Keyword(s):  
Membrane Proteins ◽  
Cell Membrane ◽  
Single Molecule ◽  
Signal Amplification ◽  
Living Cells ◽  
Single Molecule Level ◽  
Visualization Analysis ◽  
Amplification Method ◽  
Cell Membrane Proteins

A signal amplification method is developed for visualization analysis of membrane proteins on living cells at a single-molecule level.

Trophoblast Cell Membrane Interactions at Implantation

1970 ◽  
Vol 28 ◽  
pp. 310-311
Author(s):  
A. C. Enders
Keyword(s):  
Epithelial Cells ◽  
Cell Membrane ◽  
Membrane Fusion ◽  
Trophoblast Cell ◽  
Membrane Interactions ◽  
Uterine Epithelial Cells ◽  
Functional Complex ◽  
Cytotrophoblast Cells ◽  
Complex Relationships ◽  
Syncytial Trophoblast

The alteration in membrane relationships seen at implantation include 1) interaction between cytotrophoblast cells to form syncytial trophoblast and addition to the syncytium by subsequent fusion of cytotrophoblast cells, 2) formation of a wide variety of functional complex relationships by trophoblast with uterine epithelial cells in the process of invasion of the endometrium, and 3) in the case of the rabbit, fusion of some uterine epithelial cells with the trophoblast.Formation of syncytium is apparently a membrane fusion phenomenon in which rapid confluence of cytoplasm often results in isolation of residual membrane within masses of syncytial trophoblast. Often the last areas of membrane to disappear are those including a desmosome where the cell membranes are apparently held apart from fusion.

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