Whole tissue and single cell mechanics are correlated in human brain tumors

Biomechanical changes are critical for cancer progression. However, the relationship between the rheology of single cells measured ex-vivo and the living tumor is not yet understood. Here, we combined single-cell...

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A non-aggressive, highly efficient, enzymatic method for dissociation of human brain-tumors and brain-tissues to viable single-cells

BMC Neuroscience ◽

10.1186/s12868-016-0262-y ◽

2016 ◽

Vol 17 (1) ◽

Cited By ~ 15

Author(s):

Ilan Volovitz ◽

Netanel Shapira ◽

Haim Ezer ◽

Aviv Gafni ◽

Merav Lustgarten ◽

...

Keyword(s):

Brain Tumors ◽

Human Brain ◽

Single Cells ◽

Enzymatic Method ◽

Human Brain Tumors ◽

Highly Efficient ◽

Brain Tissues

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S-A-6. The Relationship between the Enzymatic Activities of Human Brain Tumors and Clinical Features

Neurologia medico-chirurgica ◽

10.2176/nmc.7.80 ◽

1965 ◽

Vol 7 ◽

pp. 80-83

Author(s):

Takao KAWASHIMA ◽

Kisaburo TAKEUCHI ◽

Masato NAKAMURA ◽

Takuro OGATA ◽

Akira NISHIMOTO

Keyword(s):

Brain Tumors ◽

Human Brain ◽

Clinical Features ◽

Enzymatic Activities ◽

Human Brain Tumors ◽

The Relationship

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Redox Imbalance and Biochemical Changes in Cancer by Probing Redox-Sensitive Mitochondrial Cytochromes in Label-Free Visible Resonance Raman Imaging

Cancers ◽

10.3390/cancers13050960 ◽

2021 ◽

Vol 13 (5) ◽

pp. 960

Author(s):

Halina Abramczyk ◽

Beata Brozek-Pluska ◽

Monika Kopec ◽

Jakub Surmacki ◽

Maciej Błaszczyk ◽

...

Keyword(s):

Cytochrome C ◽

Brain Tumors ◽

Human Brain ◽

Redox State ◽

Ductal Carcinoma ◽

Ex Vivo ◽

Raman Imaging ◽

Enhancement Effect ◽

Human Brain Tumors ◽

Mitochondrial Cytochromes

To monitor redox state changes and biological mechanisms occurring in mitochondrial cytochromes in cancers improving methods are required. We used Raman spectroscopy and Raman imaging to monitor changes in the redox state of the mitochondrial cytochromes in ex vivo human brain and breast tissues at 532 nm, 633 nm, 785 nm. We identified the oncogenic processes that characterize human infiltrating ductal carcinoma (IDC) and human brain tumors: gliomas; astrocytoma and medulloblastoma based on the quantification of cytochrome redox status by exploiting the resonance-enhancement effect of Raman scattering. We visualized localization of cytochromes by Raman imaging in the breast and brain tissues and analyzed cytochrome c vibrations at 750, 1126, 1337 and 1584 cm-1 as a function of malignancy grade. We found that the concentration of reduced cytochrome c becomes abnormally high in human brain tumors and breast cancers and correlates with the grade of cancer. We showed that Raman imaging provides additional insight into the biology of astrocytomas and breast ductal invasive cancer, which can be used for noninvasive grading, differential diagnosis.

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