A Novel TMTP1-Modified Theranostic Nanoplatform for Targeted In Vivo NIR-II Fluorescence Imaging-Guided Chemotherapy of Cervical Cancer

Near-infrared II (NIR-II, 900-1700 nm) fluorescence bioimaging with advantages of good biosafety, excellent spatial resolution, high sensitivity, and contrast has attracted great attentions in biomedical research fields. However, most of...

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A Novel TMTP1-modified Theranostic Nanoplatform for Targeted in Vivo NIR-II Fluorescence Imaging-guided Chemotherapy of Cervical Cancer

10.21203/rs.3.rs-818556/v1 ◽

2021 ◽

Author(s):

Alifu Nuernisha ◽

Rong Ma ◽

Lijun Zhu ◽

Zhong Du ◽

Shuang Chen ◽

...

Keyword(s):

Cervical Cancer ◽

Blood Vessels ◽

Fluorescence Imaging ◽

Spatial Resolution ◽

Self Assembly ◽

Tumor Targeting ◽

High Sensitivity ◽

High Definition ◽

Targeting Ability

Abstract BackgroundNear-infrared II (NIR-II, 900-1700 nm) fluorescence bioimaging with advantages of good biosafety, excellent spatial resolution, high sensitivity and contrast, has attracted great attentions in biomedical research fields. However, most nanoprobes used for NIR-II fluorescence imaging have poor tumor-targeting ability and therapeutic efficiency. To overcome these limitations, a novel NIR-II-emissive theranostic nanoplatform for imaging and treatment of cervical cancer was designed and prepared. The NIR-II-emissive dye IR-783 and chemotherapy drug doxorubicin (DOX) were encapsulated into liposomes, and the tumor-targeting peptide TMTP1 was conjugated to the surface of the liposomes to form IR-783-DOX-TMTP1 nanoparticles (NPs) via self-assembly methods.ResultsThe IR-783-DOX-TMTP1 NPs showed strong NIR-II emission, excellent biocompatibility, a long lifetime, and low toxicity. Further, high-definition NIR-II fluorescence microscopy images of ear blood vessels and intratumor blood vessels were obtained from IR-783-DOX-TMTP1 NPs-stained mice with high spatial resolution under 808 nm laser excitation. Moreover, IR-783-DOX-TMTP1 NPs showed strong tumor targeting ability and high efficiently chemotherapeutic character towards cervical tumors. ConclusionsThe novel targeting and NIR-II-emissive IR-783-DOX-TMTP1 NPs have potential in diagnosis and therapy for cervical cancer.

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Protein enhanced NIR-IIb emission of indocyanine green for functional bioimaging

10.1101/2020.05.30.125104 ◽

2020 ◽

Author(s):

Mubin He ◽

Di Wu ◽

Yuhuang Zhang ◽

Xiaoxiao Fan ◽

Hui Lin ◽

...

Keyword(s):

Indocyanine Green ◽

Fluorescence Imaging ◽

Clinical Application ◽

Spatial Resolution ◽

Near Infrared ◽

Protein Solutions ◽

Lymph System ◽

Fetal Bovine ◽

Auto Fluorescence

AbstractFluorescence imaging performed in the 1500-1700 nm spectral range (labeled as near-infrared IIb, NIR-IIb) promises high imaging contrast and spatial resolution for its little photon scattering effect and minimum auto-fluorescence. Though inorganic and organic probes have been developed for NIR-IIb bioimaging, most are in preclinical stage, hampering further clinical application. Herein, we showed that indocyanine green (ICG), an US Food and Drug Administration (FDA)-approved agent, exhibited remarkable amount of NIR-IIb emission when dissolved into different protein solutions, including human serum albumin, rat bile, and fetal bovine serum. We performed fluorescence imaging in NIR-IIb window to visualize structures of lymph system, extrahepatic biliary tract and cerebrovascular. Results demonstrated that proteins promoted NIR-IIb emission of ICG in vivo and that NIR-IIb imaging with ICG preserved higher signal-to-background ratio (SBR) and spatial resolution compared with the conventional near-infrared II (NIR-II) fluorescence imaging. Our findings confirm that NIR-IIb fluorescence imaging can be successfully performed using the clinically approved agent ICG. Further clinical application in NIR-IIb region would hopefully be carried out with appropriate ICG-protein solutions.

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Acceptor Engineering of Small Molecule Fluorophores for NIR-II Fluorescent and NIR-I Photoacoustic Imaging

Journal of Materials Chemistry B ◽

10.1039/d1tb02282b ◽

2021 ◽

Author(s):

Yaxi Li ◽

Hongli Zhou ◽

Renzhe Bi ◽

Xiuting Li ◽

Menglei Zha ◽

...

Keyword(s):

Fluorescence Imaging ◽

Small Molecule ◽

Near Infrared ◽

Photoacoustic Imaging ◽

High Sensitivity ◽

Tissue Penetration ◽

Deep Tissue ◽

Infrared Window

Fluorescence imaging in the second near-infrared window (NIR-II) has been an emerging technique in diverse in vivo applications with high sensitivity/resolution and deep tissue penetration. To date, the designing principle...

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Recent Advances in Second Near-Infrared Region (NIR-II) Fluorophores and Biomedical Applications

Frontiers in Chemistry ◽

10.3389/fchem.2021.750404 ◽

2021 ◽

Vol 9 ◽

Author(s):

Yingying Chen ◽

Liru Xue ◽

Qingqing Zhu ◽

Yanzhi Feng ◽

Mingfu Wu

Keyword(s):

Fluorescence Imaging ◽

Near Infrared ◽

High Sensitivity ◽

Infrared Region ◽

Radiation Hazard ◽

High Signal ◽

Tissue Penetration ◽

Tumor Delineation ◽

Near Infrared Region

Fluorescence imaging technique, characterized by high sensitivity, non-invasiveness and no radiation hazard, has been widely applicated in the biomedical field. However, the depth of tissue penetration is limited in the traditional (400–700 nm) and NIR-I (the first near-infrared region, 700–900 nm) imaging, which urges researchers to explore novel bioimaging modalities with high imaging performance. Prominent progress in the second near-infrared region (NIR-II, 1000–1700 nm) has greatly promoted the development of biomedical imaging. The NIR-II fluorescence imaging significantly overcomes the strong tissue absorption, auto-fluorescence as well as photon scattering, and has deep tissue penetration, micron-level spatial resolution, and high signal-to-background ratio. NIR-II bioimaging has been regarded as the most promising in vivo fluorescence imaging technology. High brightness and biocompatible fluorescent probes are crucial important for NIR-II in vivo imaging. Herein, we focus on the recently developed NIR-II fluorescent cores and their applications in the field of biomedicine, especially in tumor delineation and image-guided surgery, vascular imaging, NIR-II-based photothermal therapy and photodynamic therapy, drug delivery. Besides, the challenges and potential future developments of NIR-II fluorescence imaging are further discussed. It is expected that our review will lay a foundation for clinical translation of NIR-II biological imaging, and inspire new ideas and more researches in this field.

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Structural and Functional NIR-II Fluorescence Bioimaging in Urinary System via Clinically Approved Dye Methylene Blue

10.1101/2020.01.24.917955 ◽

2020 ◽

Author(s):

Dingwei Xue ◽

Di Wu ◽

Zeyi Lu ◽

Abudureheman Zebibula ◽

Zhe Feng ◽

...

Keyword(s):

Methylene Blue ◽

Renal Function ◽

Fluorescence Imaging ◽

Near Infrared ◽

High Sensitivity ◽

Infrared Region ◽

Great Promise ◽

Urinary System ◽

Diagnosis And Prognosis

AbstractAccurate structural and functional imaging is vital for the diagnosis and prognosis of the urinary system diseases. Near-infrared region (NIR) II fluorescence imaging has shown advantages of high sensitivity, high safety, and fast feedback compared to the conventional imaging methods but limited to its clinical applicability. Herein, we first report that in vivo NIR-II fluorescence imaging of the urinary system enabled by clinically approved and renal-clearable NIR dye methylene blue, which can not only achieve clear invasive/non-invasive urography but also noninvasively detect renal function. These results demonstrate that MB assisted NIR-II fluorescence imaging holds great promise for invasive/noninvasive structural imaging of the urinary system clinically and investigation of renal function in animal models preclinically.

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Gram-scale synthesis of a neodymium chelate as a spectral CT and second near-infrared window imaging agent for visualizing the gastrointestinal tract in vivo

Journal of Materials Chemistry B ◽

10.1039/d0tb02276d ◽

2021 ◽

Author(s):

Pengrui Zhuang ◽

Ke Xiang ◽

Xiangxi Meng ◽

Guohe Wang ◽

Ziyuan Li ◽

...

Keyword(s):

Gastrointestinal Tract ◽

Fluorescence Imaging ◽

Large Scale ◽

Near Infrared ◽

Imaging Agent ◽

Spectral Ct ◽

Green Method ◽

Infrared Window

A facile and green method was developed to fabricate Nd-DTPA on a large scale without byproducts for CT/spectral CT and NIR II fluorescence imaging of the gastrointestinal tract in vivo.

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A novel antibody targeting CD24 and hepatocellular carcinoma in vivo by near-infrared fluorescence imaging

Immunobiology ◽

10.1016/j.imbio.2015.07.010 ◽

2015 ◽

Vol 220 (12) ◽

pp. 1328-1336 ◽

Cited By ~ 15

Author(s):

Hua He ◽

Xiaojie Tu ◽

Juan Zhang ◽

Desmond Omane Acheampong ◽

Li Ding ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Fluorescence Imaging ◽

Near Infrared ◽

Near Infrared Fluorescence ◽

Antibody Targeting ◽

Near Infrared Fluorescence Imaging

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Near-infrared persistent luminescence hollow mesoporous nanospheres for drug delivery and in vivo renewable imaging

Journal of Materials Chemistry B ◽

10.1039/c6tb02674e ◽

2016 ◽

Vol 4 (48) ◽

pp. 7845-7851 ◽

Cited By ~ 18

Author(s):

Junpeng Shi ◽

Meng Sun ◽

Xia Sun ◽

Hongwu Zhang

Keyword(s):

Drug Delivery ◽

Near Infrared ◽

High Sensitivity ◽

Drug Carriers ◽

Template Method ◽

Persistent Luminescence ◽

Deep Tissue ◽

Large Capacity

Near-infrared persistent luminescence hollow mesoporous nanospheres have been synthesized via a template method. These nanospheres can be used as large capacity drug carriers and realize super long-term and high sensitivity tracking of drug delivery in deep tissue.

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