Self-assembly nanoparticles based on supramolecular-organic frameworks and temoporfin for an enhanced photodynamic therapy in vitro and in vivo

2022 ◽  
Author(s):  
Ziyue Xu ◽  
Weipeng Mao ◽  
Zizhen Zhao ◽  
Zekun Wang ◽  
Yue-Yang Liu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Self Assembly ◽  
Three Dimensional ◽  
Water Soluble ◽  
Generation Efficiency ◽  
Quenching Effect

Water-soluble three-dimensional supramolecular-organic frameworks (SOFs) and temoporfin (mTHPC) are discovered to form uniform self-assembly nanoparticles. These nanoparticles demonstrate an improved 1O2 generation efficiency due to a reduced aggregation-caused quenching effect....

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

1978 ◽  
Vol 36 (2) ◽  
pp. 434-435
Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Three Dimensional ◽  
Cytochemical Study ◽  
Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

scholarly journals Multifunctional AIE iridium (III) photosensitizer nanoparticles for two-photon-activated imaging and mitochondria targeting photodynamic therapy

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Xuzi Cai ◽  
Kang-Nan Wang ◽  
Wen Ma ◽  
Yuanyuan Yang ◽  
Gui Chen ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Water Solubility ◽  
Tissue Imaging ◽  
Ros Generation ◽  
Generation Efficiency ◽  
Deep Tissue ◽  
Two Photon ◽  
Mitochondria Targeting

AbstractDeveloping novel photosensitizers for deep tissue imaging and efficient photodynamic therapy (PDT) remains a challenge because of the poor water solubility, low reactive oxygen species (ROS) generation efficiency, serve dark cytotoxicity, and weak absorption in the NIR region of conventional photosensitizers. Herein, cyclometalated iridium (III) complexes (Ir) with aggregation-induced emission (AIE) feature, high photoinduced ROS generation efficiency, two-photon excitation, and mitochondria-targeting capability were designed and further encapsulated into biocompatible nanoparticles (NPs). The Ir-NPs can be used to disturb redox homeostasis in vitro, result in mitochondrial dysfunction and cell apoptosis. Importantly, in vivo experiments demonstrated that the Ir-NPs presented obviously tumor-targeting ability, excellent antitumor effect, and low systematic dark-toxicity. Moreover, the Ir-NPs could serve as a two-photon imaging agent for deep tissue bioimaging with a penetration depth of up to 300 μm. This work presents a promising strategy for designing a clinical application of multifunctional Ir-NPs toward bioimaging and PDT.

scholarly journals The first biantennary bacterial secondary cell wall polymer and its influence on S-layer glycoprotein assembly

2002 ◽  
Vol 368 (2) ◽  
pp. 483-494 ◽  
Author(s):  
Christian STEINDL ◽  
Christina SCHÄFFER ◽  
Thomas WUGEDITSCH ◽  
Michael GRANINGER ◽  
Irena MATECKO ◽  
...  
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Secondary Cell Wall ◽  
Water Soluble ◽  
Bacterial Cells ◽  
Cell Wall Polymer ◽  
Thaw Cycle ◽  
Secondary Cell Wall Polymer

The cell surface of Aneurinibacillus thermoaerophilus DSM 10155 is covered with a square surface (S)-layer glycoprotein lattice. This S-layer glycoprotein, which was extracted with aqueous buffers after a freeze—thaw cycle of the bacterial cells, is the only completely water-soluble S-layer glycoprotein to be reported to date. The purified S-layer glycoprotein preparation had an overall carbohydrate content of 19%. Detailed chemical investigations indicated that the S-layer O-glycans of previously established structure accounted for 13% of total glycosylation. The remainder could be attributed to a peptidoglycan-associated secondary cell wall polymer. Structure analysis was performed using purified secondary cell wall polymer—peptidoglycan complexes. NMR spectroscopy revealed the first biantennary secondary cell wall polymer from the domain Bacteria, with the structure α-d-GlcpNAc-(1→3)-β-d-ManpNAc-(1→4)-β-d-GalpNAc-(1→3)-α-d-GlcpNAc-(1→3)-β-d-ManpNAc-(1→4)-β-d-GalpNAc-(1→3)-α-d-GlcpNAc-(1→4)-[α-d-GlcpNAc-(1→3)-β-d-ManpNAc-(1→4)-β-d-GalpNAc-(1→3)-α-d-GlcpNAc-(1→3)-β-d-ManpNAc-(1→4)-β-d-GalpNAc-(1→3)-α-d-GlcpNAc-(1→3)]-β-d-ManpNAc-(1→3)-α-d-GlcpNAc-(1→3)-β-d-ManpNAc-(1→3)-α-d-GlcpNAc-(1→3)-α-d-GlcpNAc-(1→O)-PO2--O-PO2--(O→6)-MurNAc- (where MurNAc is N-acetylmuramic acid). The neutral polysaccharide is linked via a pyrophosphate bond to the C-6 atom of every fourth N-acetylmuramic acid residue, in average, of the A1γ-type peptidoglycan. In vivo, the biantennary polymer anchored the S-layer glycoprotein very effectively to the cell wall, probably due to the doubling of motifs for a proposed lectin-like binding between the polymer and the N-terminus of the S-layer protein. When the cellular support was removed during S-layer glycoprotein isolation, the co-purified polymer mediated the solubility of the S-layer glycoprotein in vitro. Initial crystallization experiments performed with the soluble S-layer glycoprotein revealed that the assembly property could be restored upon dissociation of the polymer by the addition of poly(ethylene glycols). The formed two-dimensional crystalline S-layer self-assembly products exhibited the same lattice symmetry as observed on intact bacterial cells.

scholarly journals JUSTIFICATION OF THE APPLICATION OF THE METHOD OF THREE-DIMENSIONAL PHOTODYNAMIC THERAPY OF DISEASES AND PROCESSES OF MICROBIAL AND NEOPLASTIC NATURE IN CLINICAL GYNECOLOGY

2017 ◽  
Vol 4 (4) ◽  
pp. 194-200
Author(s):  
M. T Aleksandrov ◽  
Vladimir M. Zuev ◽  
Yu. I Pimancheva ◽  
E. P Pashkov ◽  
G. E Bagramova
Keyword(s):  
Photodynamic Therapy ◽  
Experimental Studies ◽  
Three Dimensional ◽  
The Body ◽  
Myelogenous Leukemia ◽  
Ehrlich Carcinoma ◽  
Neoplastic Processes ◽  
Medical Diagnostic

There were executed experimental studies on test subjects of microbial (Staphylococcus aureus and Pseudomonas aeruginosa) and neoplastic nature (in vitro - suspension of cells of the line of chronic myelogenous leukemia K562 in a volume of 60 μl and in an amount of 60 ± 1 × 103, in vivo - mice infected with Ehrlich carcinoma) on the substantiation the use of chlorophyll-containing drugs activated for photodynamic therapy (PDT) outside the biological object. No additional PDT activation of the drug was performed.The high bactericidal (on the test objects of microbes) and anti-tumor PDT efficacy of chlorophyll-containing preparations activated outside the organism was substantiated, with their subsequent administration per os and accumulation in practically all organs and tissues of the body was validated. The developed medical diagnostic technology in its clinical application has proved its effectiveness in women with inflammatory and/or neoplastic processes of the pelvic organs. The used equipment and preparation are approved for clinical use.

scholarly journals Photodynamic Therapy-Mediated Immune Responses in Three-Dimensional Tumor Models

2021 ◽  
Vol 22 (23) ◽  
pp. 12618
Author(s):  
Nkune Williams Nkune ◽  
Nokuphila Winifred Nompumelelo Simelane ◽  
Hanieh Montaseri ◽  
Heidi Abrahamse
Keyword(s):  
Photodynamic Therapy ◽  
Immune Responses ◽  
Three Dimensional ◽  
Tumor Model ◽  
Experimental Models ◽  
Cellular Heterogeneity ◽  
Tumor Models ◽  
Cellular Environment

Photodynamic therapy (PDT) is a promising non-invasive phototherapeutic approach for cancer therapy that can eliminate local tumor cells and produce systemic antitumor immune responses. In recent years, significant efforts have been made in developing strategies to further investigate the immune mechanisms triggered by PDT. The majority of in vitro experimental models still rely on the two-dimensional (2D) cell cultures that do not mimic a three-dimensional (3D) cellular environment in the human body, such as cellular heterogeneity, nutrient gradient, growth mechanisms, and the interaction between cells as well as the extracellular matrix (ECM) and therapeutic resistance to anticancer treatments. In addition, in vivo animal studies are highly expensive and time consuming, which may also show physiological discrepancies between animals and humans. In this sense, there is growing interest in the utilization of 3D tumor models, since they precisely mimic different features of solid tumors. This review summarizes the characteristics and techniques for 3D tumor model generation. Furthermore, we provide an overview of innate and adaptive immune responses induced by PDT in several in vitro and in vivo tumor models. Future perspectives are highlighted for further enhancing PDT immune responses as well as ideal experimental models for antitumor immune response studies.

scholarly journals Synthesis of Water-Soluble Iridium (III)-Containing Nanoparticles for Biological Applications

2015 ◽  
Vol 2015 ◽  
pp. 1-7
Author(s):  
Huanzhi Hou ◽  
Pengfei Sun ◽  
Quli Fan ◽  
Xiaomei Lu ◽  
Cheng Xue ◽  
...  
Keyword(s):  
Self Assembly ◽  
Laser Scanning ◽  
Morphological Characterization ◽  
Fluorescent Imaging ◽  
Water Soluble ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Biological Applications

Water-soluble nanoparticles (Ir/PGlc-NP, Ir/β-1,3-glucan-NP) based on water-soluble glycopolymers (PGlc),β-1,3-glucan polysaccharide, and conjugated phosphorescent Ir (III) complexes were successfully synthesized by self-assembly. The obtained nanoparticles have good spherical morphological characterization with a mean diameter of 50 nm measured by TEM. Ir/PGlc-NP and Ir/β-1,3-glucan-NP showed the same emission maxima at 565 nm in aqueous solution and both caused effective apoptosis and death of HepG2 and Hela cells after being irradiated at 445 nm for 30 min in vitro. Fluorescence cellular imaging was conducted by confocal laser scanning microscopy (CLSM) using HepG2 cells as the model cell in which the nanoparticles had successfully entered into the cytoplasm with high brightness. Furthermore, after injecting the nanoparticles into live mice in vivo, the real-time fluorescence imaging as well as the nanoparticles distribution in organs at 24 hours after administration indicated that these nanoparticles can serve as fluorescent imaging contrast for further biological applications.

Programmable in vitro Co-Encapsidation of Guest Proteins Inside Protein Nanocages

2018 ◽  
Author(s):  
Noor H. Dashti ◽  
Rufika S. Abidin ◽  
Frank Sainsbury
Keyword(s):  
Self Assembly ◽  
Mammalian Cells ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Super Resolution ◽  
Cell Engineering ◽  
Particle Analysis ◽  
Protein Cages

Bioinspired self-sorting and self-assembling systems using engineered versions of natural protein cages have been developed for biocatalysis and therapeutic delivery. The packaging and intracellular delivery of guest proteins is of particular interest for both <i>in vitro</i> and <i>in vivo</i> cell engineering. However, there is a lack of platforms in bionanotechnology that combine programmable guest protein encapsidation with efficient intracellular uptake. We report a minimal peptide anchor for <i>in vivo</i> self-sorting of cargo-linked capsomeres of the Murine polyomavirus (MPyV) major coat protein that enables controlled encapsidation of guest proteins by <i>in vitro</i> self-assembly. Using Förster resonance energy transfer (FRET) we demonstrate the flexibility in this system to support co-encapsidation of multiple proteins. Complementing these ensemble measurements with single particle analysis by super-resolution microscopy shows that the stochastic nature of co-encapsidation is an overriding principle. This has implications for the design and deployment of both native and engineered self-sorting encapsulation systems and for the assembly of infectious virions. Taking advantage of the encoded affinity for sialic acids ubiquitously displayed on the surface of mammalian cells, we demonstrate the ability of self-assembled MPyV virus-like particles to mediate efficient delivery of guest proteins to the cytosol of primary human cells. This platform for programmable co-encapsidation and efficient cytosolic delivery of complementary biomolecules therefore has enormous potential in cell engineering.

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