Characterization of membrane polypeptides from pea leaf peroxisomes involved in superoxide radical generation

Abstract Objectives It has been a long time since seaweeds have been used for food ingredients in Asian countries. Recently, a body of research has revealed the health benefits of bioactive compounds in seaweeds, especially their antioxidant capacities. Although numerous seaweeds inhabit in the ocean, only a small percentage has been explored for functional food. Therefore, the purpose of this study was to analyze the antioxidant capacities of various seaweeds grown off the Korean coast to screen for the potential functional food sources. Methods Ten ethanol extracts of Korean seaweeds were provided by the National Marine Biodiversity Institute of Korea, which included Scytosiphon gracilis, Scytosiphon lomentaria, Sargassum muticum, Sargassum confusum, Petrospongium rugosum, Sargassum fusiforme, Petalonia fascia, Sargassum nigrifolium, Ishige foliacea, and Myelophycus simplex. Hundred mg/L of samples in 80% methanol was used to measure their ABTS, DPPH, and superoxide radical scavenging activities. Mg vitamin C equivalent antioxidant capacity (VCEAC)/100 mg was used to express the ABTS and DPPH radical scavenging capacities. For the superoxide radical scavenging capacity, inhibition rate of superoxide radical generation (%) was calculated. Results Among the ten seaweeds, S. nigrifolium and I. foliacea exhibited the most significant radical scavenging capacities. DPPH radical scavenging capacities of I. foliacea and S. nigrifolium were 122.4 mg VCEAC/100 mg and 95.8 mg VCEAC/100 mg, respectively. For ABTS radical scavenging capacity, I. foliacea exhibited 178.5 mg VCEAC/100 mg, followed by S. nigrifolium as 80.9 mg VCEAC/100 mg. I. foliacea inhibited about 68% of superoxide radical generation followed by S. nigrifolium (40.6%) while vitamin C as positive control inhibited about 28.9% of superoxide radical generation. The antioxidant capacities measured by the three assays were positively correlated with each other. Conclusions The current study explored total antioxidant capacities of various Korean seaweeds and found I. foliacea and S. nigrifolium as the most potential antioxidant-rich food resources. Further research would be warranted to investigate bioactive compounds from S. nigrifolium and I. foliacea. Funding Sources This work was supported by the Pukyong National University Research Fund in 2018.

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Adenosine inhibition of neutrophil damage during reperfusion does not involve KATP-channel activation

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.1997.273.4.h1677 ◽

1997 ◽

Vol 273 (4) ◽

pp. H1677-H1687 ◽

Cited By ~ 5

Author(s):

Zhi-Qing Zhao ◽

James C. Todd ◽

Hiroki Sato ◽

Xin-Liang Ma ◽

J. Vinten-Johansen

Keyword(s):

Infarct Size ◽

Superoxide Radical ◽

Receptor Activation ◽

Polymorphonuclear Neutrophils ◽

Vehicle Group ◽

Myeloperoxidase Activity ◽

Dependent Manner ◽

Glucose Levels ◽

Radical Generation ◽

Dose Dependent

This study tests the hypothesis that cardioprotection exerted by adenosine A2-receptor activation and neutrophil-related events involves stimulation of ATP-sensitive potassium (KATP) channels on neutrophils during reperfusion. The adenosine A2 agonist CGS-21680 (CGS) inhibited superoxide radical generation from isolated rabbit polymorphonuclear neutrophils (PMNs) in a dose-dependent manner from 17.7 ± 2.1 to 7.4 ± 1.3 nmol/5 × 106 PMNs ( P < 0.05). Pinacidil, a KATP-channel opener, partially inhibited superoxide radical production, which was completely reversed by glibenclamide (Glib). Incremental doses of Glib in combination with CGS (1 μM) did not alter CGS-induced inhibition of superoxide radical generation. CGS significantly reduced PMN adherence to the endothelial surface of aortic segments in a dose-dependent manner from 189 ± 8 to 50 ± 6 PMNs/mm2( P < 0.05), which was also not altered by incremental doses of Glib. Infusion of CGS (0.025 mg/kg) before reperfusion reduced infarct size from 29 ± 2% in the Vehicle group to 15 ± 1% in rabbits undergoing 30 min of ischemia and 120 min of reperfusion ( P< 0.05). Glib (0.3 mg/kg) did not change the infarct size (28 ± 2%) vs. the Vehicle group and did not attenuate infarct size reduction by CGS (16 ± 1%). Glib did not change blood glucose levels. Cardiac myeloperoxidase activity was decreased in the ischemic tissue of the CGS group (0.15 ± 0.03 U/100 mg tissue) compared with the Vehicle group (0.37 ± 0.05 U/100 mg tissue; P < 0.05). We conclude that adenosine A2 activation before reperfusion partially reduces infarct size by inhibiting neutrophil activity and that this effect does not involve KATP-channel stimulation.

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Effect of Adriamycin on superoxide radical generation in isolated heart mitochondria

BIOPHYSICS ◽

10.1134/s0006350907060097 ◽

2007 ◽

Vol 52 (6) ◽

pp. 582-586 ◽

Cited By ~ 2

Author(s):

I. V. Sviryaeva ◽

E. K. Ruuge ◽

K. B. Shumaev

Keyword(s):

Superoxide Radical ◽

Isolated Heart ◽

Heart Mitochondria ◽

Radical Generation

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Characterization of hydroxyl radical generation in the striatum of free-moving rats due to carbon monoxide poisoning, as determined by in vivo microdialysis

Brain Research ◽

10.1016/j.brainres.2004.05.047 ◽

2004 ◽

Vol 1016 (2) ◽

pp. 281-284 ◽

Cited By ~ 24

Author(s):

Shuichi Hara ◽

Toshiji Mukai ◽

Kunihiko Kurosaki ◽

Fumi Kuriiwa ◽

Takahiko Endo

Keyword(s):

Carbon Monoxide ◽

Hydroxyl Radical ◽

Carbon Monoxide Poisoning ◽

In Vivo Microdialysis ◽

Radical Generation

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Iron-induced changes in nitric oxide and superoxide radical generation in rat liver after lindane or thyroid hormone treatment

Toxicology Letters ◽

10.1016/s0378-4274(00)00295-2 ◽

2001 ◽

Vol 119 (2) ◽

pp. 87-93 ◽

Cited By ~ 21

Author(s):

Pamela Cornejo ◽

Gladys Tapia ◽

Susana Puntarulo ◽

Mónica Galleano ◽

Luis A. Videla ◽

...

Keyword(s):

Nitric Oxide ◽

Thyroid Hormone ◽

Rat Liver ◽

Superoxide Radical ◽

Hormone Treatment ◽

Radical Generation ◽

Induced Changes

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Superoxide Radical Generation, NADPH Oxidase Activity, and Cytochrome P-450 Content of Rat Liver Microsomal Fractions in an Experimental Hyperthyroid State: Relation to Lipid Peroxidation*

Endocrinology ◽

10.1210/endo-117-2-496 ◽

1985 ◽

Vol 117 (2) ◽

pp. 496-501 ◽

Cited By ~ 109

Author(s):

VIRGINIA FERNANDEZ ◽

XIMENA BARRIENTOS ◽

KATINA KIPREOS ◽

ALFONSO VALENZUELA ◽

LUIS A. VIDELA

Keyword(s):

Lipid Peroxidation ◽

Nadph Oxidase ◽

Rat Liver ◽

Oxidase Activity ◽

Superoxide Radical ◽

Nadph Oxidase Activity ◽

Hyperthyroid State ◽

Radical Generation ◽

Cytochrome P 450

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Mitochondrial respiratory activity and superoxide radical generation in the liver, brain and heart after chronic ethanol intake

Biochemical Pharmacology ◽

10.1016/0006-2952(94)90312-3 ◽

1994 ◽

Vol 47 (10) ◽

pp. 1827-1833 ◽

Cited By ~ 33

Author(s):

Catherine Ribiere ◽

Isabelle Hininger ◽

Chantal Saffar-Boccara ◽

Dominique Sabourault ◽

Roger Nordmann

Keyword(s):

Superoxide Radical ◽

Respiratory Activity ◽

Ethanol Intake ◽

Chronic Ethanol ◽

Radical Generation ◽

Mitochondrial Respiratory Activity ◽

Mitochondrial Respiratory

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Superoxide- and no-dependent mechaniSmS of antitumor and antimetaStatic effect of L-arginine hydrochLoride and coenzyme Q10

Experimental Oncology ◽

10.31768/2312-8852.2016.38(1):31-35 ◽

2016 ◽

Vol 38 (1) ◽

pp. 31-35 ◽

Cited By ~ 3

Author(s):

A P Burlaka ◽

I I Ganusevich ◽

V V Golotiuk ◽

A V Vovk ◽

S M Lukin

Keyword(s):

Tumor Growth ◽

Lewis Lung Carcinoma ◽

Superoxide Radical ◽

Tumor Growth Inhibition ◽

Low Doses ◽

Lewis Lung ◽

Combined Use ◽

Arginine Hydrochloride ◽

Radical Generation ◽

High Doses

Aim: To study the redox-dependent mechanism of antiradical, antitumor and antimetastatic action of L-arginine hydrochloride (L-Arg) and coenzyme Q10 (CoQ10) in vivo. Materials and Methods: The study was performed on С57Вl mice with transplanted Lewis lung carcinoma treated by intraperitoneal injections of L-Arg at low or high doses (60 and 360 mg/kg body weight), CoQ10 (0.2 and 1.2 mg/kg body weight) or their combinations. Electron paramagnetic resonance was applied for analysis of mitochondrial electron transport chain, СoQ10 levels, free iron (FI), the level of NO, and the rate of superoxide radical generation. The activity of matrix metalloproteinase (MMP)-2 and -9 in tumor tissue was determined by zymography method in polyacrylamide gel. Results: Administration of L-Arg at high doses caused an inhibition of tumor growth by 48 ± 8.0%, increase of superoxide radical generation rate and NO levels to a value of 1.23 ± 0.14 аnd 2.26 ± 0.31 nm/g tissue · min, and decreased activity of MMP-2 and -9 (3.55 ± 0.8 and 4.8 ± 1.0 r.u., respectively). Treatment with L-Arg at low doses stimulated tumor growth and increased the levels of MMP-2 and -9 activities (8.44 ± 2.7 and 9.8 ± 3.1 r.u., respectively). Administration of СoQ10 at high doses significantly decreased superoxide radical generation rate to the values of 0.44 ± 0.09 nm/g tissue · min, levels of free iron and NO, and caused tumor growth inhibition by 54 ± 11.3%. The combined use of L-Arg and СoQ10 at high doses caused tumor growth inhibition by 51 ± 7.4% compared to Lewis lung carcinoma-bearing untreated animals (р < 0.05). Conclusions: Administration of L-Arg and СoQ10 caused the dose-dependent effect on the rate of generation of superoxide radicals, level of ubisemyquinone, complexes NOFeS-proteins, levels of FI and NO. L-Arg at low doses positively modulated MMP-9 activity that promoted tumor progression. Upon combined use of L-Arg and СoQ10, superoxide radicals and NO form the redox state that causes decrease of MMP-2, -9 activities with consequent inhibition of tumor invasion and metastasis.

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