scholarly journals Thermal stability of ribosomal ribonucleic acid from baby hamster kidney cells

1966 ◽  
Vol 101 (3) ◽  
pp. 721-726 ◽  
Author(s):  
SJ Martin
Keyword(s):  
Heat Treatment ◽  
Thermal Stability ◽  
Ribonucleic Acid ◽  
Sedimentation Coefficient ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Similar Treatment ◽  
Ribosomal Ribonucleic Acid ◽  
Thermal Stability Of ◽  
Baby Hamster Kidney Cells

1. RNA has been prepared from baby hamster kidney cells by extraction with a phenol-EDTA mixture and further purified by passing through a column of Sephadex G-25 that had been equilibrated with water. 2. Aging of the total RNA extracts at 4 degrees or heating at 95 degrees followed by rapid cooling caused a conversion of 28s RNA into material sedimenting in sucrose gradients at approx. 18s. 3. When heated RNA was re-extracted with phenol the sedimentation profile was not returned to that of the unheated RNA. 4. The 28s and 18s RNA fractions were collected separately from sucrose gradients by precipitation with 2vol. of ethanol and passed through a Sephadex G-25 column equilibrated with water. 5. Heat treatment of purified 28s RNA at 95 degrees caused the sedimentation coefficient to increase to approx. 40s, whereas similar treatment of 18s RNA caused no significant increase. If the RNA was heated before the Sephadex G-25 treatment the sedimentation coefficient of the 28s and 18s RNA decreased to approx. 12s and 8s. 6. Heating mixtures of purified 28s and 18s RNA at 95 degrees caused some aggregation of 18s material with the 28s fraction.

scholarly journals Synthesis of ribonucleic acid in baby-hamster kidney cells in the presence of actinomycin D

1967 ◽  
Vol 105 (3) ◽  
pp. 979-985 ◽  
Author(s):  
S J Martin ◽  
F. Brown
Keyword(s):  
Ribonucleic Acid ◽  
Base Composition ◽  
Actinomycin D ◽  
Exponential Phase ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Rich Medium ◽  
Prolonged Incubation ◽  
Rna Molecules ◽  
Baby Hamster Kidney Cells

1. RNA molecules with sedimentation values in sucrose gradients of 12–20s are synthesized in baby-hamster kidney cells even after prolonged incubation in medium containing 1μg. of actinomycin D/ml. 2. The rate of formation of this RNA is dependent on the age of the cultures and is greatest during the exponential phase of growth. 3. Growth of cells on nutritionally poor medium causes degradation and inhibits the synthesis of these RNA fractions. 4. Replacement of the nutritionally poor medium with a rich medium stimulates the synthesis of actinomycin-resistant RNA. This stimulation is blocked by cycloheximide. 5. The base composition of this RNA is characterized by low cytidine and high guanosine values.

Turbomolecular Pumped Electron Microscopy of Rubella Virus (In BHK Celis)

1968 ◽  
Vol 26 ◽  
pp. 204-205
Author(s):  
A. B. Taylor ◽  
G. C. Cole ◽  
M. A. Holcomb ◽  
C. A. Baechler
Keyword(s):  
Electron Microscopy ◽  
Rubella Virus ◽  
Phosphate Buffer ◽  
Fetal Calf Serum ◽  
Calf Serum ◽  
Basal Medium ◽  
Kidney Cells ◽  
Baby Hamster Kidney ◽  
Input Multiplicity ◽  
Baby Hamster Kidney Cells

An aliquot from a continuous fermenter culture of baby hamster kidney cells (BHK-21 Clone PD-4) (Wistar) maintained in Ca free Eagle's Basal Medium containing 2% Kaolin adsorbed fetal calf serum was planted in spinner flasks at 300,000 cells per ml, total volume 600 ml. After equilibration for one day at 35°C to insure that cells were in log phase, the culture was infected with the M-33-AGMK25 BHK-219 strain of rubella at an input multiplicity of about 6 TCID50 per cell. The virus was identified with specific rubella antiserum.Preliminary experiments had shown that such cultures would reach a peak or plateau HA titer of approximately 1:64, 24 hrs after inoculation and would continue to yield virus for 6 to 12 days. One hundred ml aliquot harvests were withdrawn daily and the culture was returned to volume with growth medium and incubation continued. The harvested cells were spun down rapidly at 2500 rpm per 15 mins., fixed in 3.7% gluteraldehyde in Ca free phosphate buffer saline, and post fixed in osmium tetraoxide. After dehydration, the cells were embedded in Epon 812 and cured approximately 20 hrs at 60°C.

Thermal stability of phase-separated nanograin structure during heat treatment

2021 ◽  
Vol 875 ◽  
pp. 160055
Author(s):  
Hua Guo ◽  
Fawei Tang ◽  
Yong Liu ◽  
Zhi Zhao ◽  
Hao Lu ◽  
...  
Keyword(s):  
Heat Treatment ◽  
Thermal Stability ◽  
Thermal Stability Of
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