scholarly journals Ability of human chorionic gonadotropin β-subunit to inhibit the steroidogenic response to lutropin

1979 ◽  
Vol 180 (3) ◽  
pp. 573-578 ◽  
Author(s):  
R R Dighe ◽  
K Muralidhar ◽  
N R Moudgal
Keyword(s):  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Beta Subunit ◽  
Alpha Subunit ◽  
Immature Rat ◽  
Progesterone Production ◽  
Pregnant Mare Serum Gonadotropin ◽  
Serum Gonadotropin ◽  
Constant Dose ◽  
Human Chorionic Gonadotropin Beta

Ability of the beta-subunit of human chorionic gonadotropin to inhibit the response to lutropin (luteinizing hormone, LH) was tested in the immature rat ovarian system and pregnant-mare-serum-gonadotropin-primed rat ovarian system with progesterone production being used as the response. Human chorionic gonadotropin beta-subunit was found to inhibit human and ovine lutropin-stimulated progesterone production. At a constant dose of lutropin, inhibition was dependent on the concentration of beta-subunit. When concentration of the beta-subunit was kept constant at 5.0 microgram/ml and the concentration of lutropin was varied, the inhibition was maximum at the saturating concentration of the native hormone. The alpha-subunit of the human chorionic gonadotropin did not inhibit the response to lutropin. The lutropin/beta-subunit ratio required to produce an inhibition of response was much lower than that required to bring about an observable inhibition of binding.

Discordant Results in Human Chorionic Gonadotropin Assays

1992 ◽  
Vol 38 (2) ◽  
pp. 263-270 ◽  
Author(s):  
Laurence A Cole ◽  
Andrew Kardana
Keyword(s):  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Terminal Segment ◽  
Beta Subunit ◽  
Alpha Subunit ◽  
Individual Sample ◽  
Serum Samples ◽  
Subunit C ◽  
Core Fragment ◽  
Alpha Type

Abstract Discordance has been reported in human chorionic gonadotropin (hCG) concentrations measured by different immunoassay kits. We examined the results for 40 serum samples assayed with 10 different hCG immunoassay kits. Results varied considerably. Individual sample results varied by as much as 58-fold. Average results for different kits varied by as much as 1.4-fold for pregnancy (20 samples) and 2.2-fold for trophoblast disease (20 samples) serum. We investigated the causes of this discordance. hCG or hCG beta are general names for mixtures of hCG, hCG alpha, or hCG beta immunoreactive molecules in serum. These mixtures include regular hCG, nicked hCG (missing peptide linkages at beta 44-45 or beta 47-48), carbohydrate variants of hCG, hCG missing the beta-subunit C-terminal segment, free beta-subunit, beta-core fragment, and free alpha-subunit. We prepared standards for each of these major variants and measured their reactivities in the 10 hCG immunoassay kits. Free beta-subunit reactivity varied from nonrecognition (anti-beta:anti-alpha type kits; Hybritech Tandem-R and others) to overrecognition (one kit had five-fold greater affinity for free beta than for hCG). Kits with antibodies to beta-subunit C-terminal segment (Organon NML and others) failed to recognize hCG missing this segment, a component of serum hCG in trophoblast disease. Kits with anti-hCG antibodies (Serono MAIA-clone and others) had minimal recognition of nicked hCG (12%), a component of all serum hCG samples, and consistently gave the lowest values with all serum samples. We conclude that differences in recognition of nicked hCG, free beta, and these other hCG variants cause discordance in hCG immunoassay results.

Identification and selective destruction of shared epitopes in human chorionic gonadotropin beta subunit

1996 ◽  
Vol 31 (1-2) ◽  
pp. 21-36 ◽  
Author(s):  
Alison M. Jackson ◽  
Thomas Klonisch ◽  
Adrian J. Lapthorn ◽  
Peter Berger ◽  
Neil W. Isaacs ◽  
...  
Keyword(s):  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Beta Subunit ◽  
Selective Destruction ◽  
Shared Epitopes ◽  
Human Chorionic Gonadotropin Beta

scholarly journals Specific detection of type II human chorionic gonadotropin beta subunit produced by trophoblastic and neoplastic cells

2015 ◽  
Vol 444 ◽  
pp. 92-100 ◽  
Author(s):  
L. Aldaz-Carroll ◽  
S. Richon ◽  
V. Dangles-Marie ◽  
M. Cocquebert ◽  
T. Fournier ◽  
...  
Keyword(s):  
Chorionic Gonadotropin ◽  
Human Chorionic Gonadotropin ◽  
Beta Subunit ◽  
Type Ii ◽  
Specific Detection ◽  
Neoplastic Cells ◽  
Human Chorionic Gonadotropin Beta
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