Superovulatory responses using pregnant mare serum gonadotropin hormone in Murrah buffalo cows

Superovulation plays an important role in embryo transfer program. A preliminary study carried out in the Research Institute for Animal Production evaluated superovulatory responses in Murrah buffalo cows using pregnant mare serum gonadotropin (PMSG) hormone. The three buffalo cows were estrus synchronized using 5 ml prostaglandin (PGF) twice, with an interval of 11 days. PMSG was injected intra-muscularly 3000 IU on day-10 after estrus. Prostaglandins were administered 48 hours after PMSG injection. Fixed-Time artificial insemination (FTAI) was carried out at 72 hours after the last PGF treatment. Administration of hCG 2 ml/head was given at the time of FTAI. A non-surgery flushing was performed on day 6 after FTAI. Parameters observed using ultrasonography (USG) were diameter of follicle (DFL), total follicle (TFL), and number embryos (NE). Data were analyzed descriptively. The mean of DFL before PMSG treatment was 8.2 mm and after PMSG treatment was 12.5 mm. The mean of TFL before PMSG treatment was 7.7 and after PMSG treatment was 16.1. The NE obtained was one degenerative embryo. Superovulation using PMSG increased TCL and DFL. It can be concluded that the Murrah buffalo cows superovulated by PMSG showed a good response but no transferable embryo was found.

Determining the Effects of Serial Injections of Pregnant Mare Serum Gonadotropin on Plasma Testosterone Concentrations, Testicular Dynamics, and Semen Production in Leopard Geckos (Eublepharis macularius)

Reptiles are highly susceptible to anthropogenic activities as a result of their narrow geographical ranges and habitat specialization, making them a conservation concern. Geckos represent one of the mega-diverse reptile lineages under pressure; however, limited assisted reproductive technologies currently exist for these animals. Exogenous pregnant mare serum gonadotropin (PMSG) has been found to exhibit follicle stimulating hormone-like action and has been routinely used to alter reproductive hormones of vertebrates in assisted reproductive protocols. The purpose of this study was to determine the effects of serial injections of 20 IU and 50 IU PMSG on circulating testosterone concentrations, testicular dynamics, and semen production in a model species of gecko. Twenty-four captive-bred, adult, male leopard geckos (Eublepharis macularius) were divided into three treatment groups and administered a once-weekly injection of either PMSG or saline for a total of nine weeks. Ultrasonographic testicular measurements, electrostimulation for semen collection, and venipuncture were performed on days 0, 21, 42, and 63. Right unilateral orchidectomies and epididymectomies were performed in all animals on day 63; tissues were submitted for histopathology. PMSG treated geckos had significantly higher testicular volumes and weights, spermatozoa motility, and spermatozoa concentrations compared with controls. However, there were no significant differences in testosterone concentrations by treatment or time. Under the conditions outlined, PMSG is effective at stimulating spermatogenesis and increasing testicular size, but not effective at increasing testosterone concentrations in the leopard gecko between October–December in the Northern hemisphere.

Eremomastax speciosa potentializes the PMSG-inducing effect on some physiological and biochemical parameters in PMSG-primed immature rats

SummaryThe present study evaluated the effect of the aqueous extract from leaves of E. speciosa on some physiological and biochemical parameters of reproduction and the onset of puberty in pregnant mare serum gonadotropin (PMSG)-primed immature female rats. High pressure liquid chromatography (HPLC) was used to quantify the phenolic compounds in the methanol/methylene chloride (1:1) extract, the ethanolic and ethyl acetate fractions and the aqueous residue of E. speciosa. E. speciosa (0, 8, 32 or 64 mg/kg) were administered for 15 days to 24 non-PMSG-primed and 24 primed rats with 0.01 IU of PMSG. At the end of the treatment period, animal were sacrificed and their body, ovarian, uterine weight, ovarian protein or cholesterol level, as well as data on puberty onset were recorded. Of the 16 polyphenolic compounds quantitatively revealed in the extracts and fractions of E. speciosa after HPLC analysis, quercetin, rutin, apigenin and eugenol were the most abundant. Non-primed rats showed a significant increase (P < 0.05) in the uterine relative weight at the dose of 8 mg/kg when compared with the other treatments. The uterine proteins and the ovarian cholesterol (P < 0.05), respectively, showed a reduction at doses of 64 mg/kg and 32 mg/kg in non-primed rats. However in PMSG-primed rats, a significant decrease (P < 0.05) was observed in ovarian cholesterol at 64 mg/kg. In conclusion, E. speciosa potentializes the PMSG-inducing effect on folliculogenesis in PMSG-primed rats.

ẢNH HƯỞNG CỦA KÍCH DỤC TỐ LÊN ĐỘNG THÁI SINH DỤC VÀ KHẢ NĂNG SINH SẢN CỦA CẦY VÒI HƯƠNG (Paradoxurus hermaphroditus) CHẬM ĐỘNG DỤC

Nghiên cứu này được thực hiện để đánh giá hiệu quả của kích dục tố (PMSG – pregnant mare serum gonadotropin, HCG – human chorionic gonadotropin) lên thời gian xuất hiện, thời gian kéo dài động dục và hiệu suất sinh sản trên đối tượng cầy vòi hương chậm động dục. Thí nghiệm được tiến hành trên 54 cá thể cầy vòi hương cái và 42 cá thể cầy đực tại trung tâm Công nghệ sinh học tỉnh Đồng Nai và trang trại động vật hoang dã Thanh Long. Chế phẩm PMSG/HCG (Gestavet –Vương quốc Anh) được tiêm bắp theo 3 công thức CT1: 20 IU PMSG + 10 IU HCG; CT2: 30 IU PMSG + 15 IU HCG; CT3: 40 IU PMSG + 20 IU HCG. Kết quả cho thấy thời gian xuất hiện động dục trung bình từ 1,1 ngày đến 2,6 ngày sau khi tiêm kích dục tố. CT3 có tác động động dục sớm nhất. Thời gian động dục kéo dài từ 2,9 đến 3,9 ngày; thời gian động dục ngắn nhất ở CT1 và dài nhất ở CT3 (p < 0,05). Tỉ lệ cầy động dục và mang thai tăng so với đối chứng ở tất cả các nhóm cầy thí nghiệm. Số con sinh ra trung bình từ 1,67 đến 3,25 con/lứa và cao nhất ở CT3 (p < 0,05). Khối lượng con sơ sinh từ 94,02 đến 97,25 g/cá thể. Kết quả cho thấy có thể áp dụng công thức CT3 có hiệu quả cho việc điều trị sự chậm lên giống lần đầu ở cầy tơ và trì hoãn động dục lại ở cầy cái sau khi sinh con.

Performance of Pregnant Mare Serum Gonadotropin and Vitamin Mix in Inducing Striped Catfish Pangasianodon hypophthalmus Rematuration

The research aimed to assess the performance of Pregnant Mare Serum Gonadotropin (PMSG) and Human Chorionic Gonadotropin (HCG) hormone injection combined with vitamin mix to induce the rematuration of Striped catfish Pangasianodon hypophthalmus broodstock. Three PMSG and HCG treatments of 5 and 2,5 IU; 10 and 5 IU;also 20 and 10 IU/kg of broodstock body weight dosage combined with vitamin mix addition to fish feed and one control was used with four replications. The broodstock was reared for four weeks and weekly sampling for GR and SGR as well as hormone injection were performed. For the following two weeks, the broodstock was observed for maturation. Artificial spawning was conducted for broodstock reaching gonadal maturity. Statistic analysis of observed variables showed insignificant differences among the three treatments in rematuration parameters. The rematuration rate resulted in a 100% pregnancy. Broodstock treated with 10 IU PMSG and 5 IU HCG/kg of spawner body weight was able to reach maturity and spawned with the larval survival rate on the fourth day at 84,81%, thus it can be concluded that 10 IU PMSG and 5 IU HCG/kg of body weight as the best dosage.

PERTUMBUHAN DAN PEMATANGAN GONAD IKAN GIRU Amphiprion clarkii YANG DIBERI PAKAN MENGANDUNG HORMON OODEV

Pertumbuhan dan pematangan gonad memainkan peranan penting dalam budidaya ikan. Untuk memperoleh benih yang berkualitas, induk harus benar benar matang gonad dan memiliki ukuran tubuh yang sesuai. Umum digunakan dalam kegiatan budidaya ikan, hormon Oodev dapat merangsang pematangan gonad karena hormon Oodev ini mengandung pregnant mare serum gonadotropin dan antidopamin yang sama-sama berperan dalam pematangan gonad. Sekalipun hormon ini begitu populer di bidang budidaya ikan, pengaruh hormon ini terhadap pertumbuhan tubuh dan pematangan gonad terhadap ikan giru (Amphiprion clarkii) belum banyak diketahui. Penelitian ini bertujuan untuk mengevaluasi pengaruh dan menentukan dosis pemberian hormon Oodev dalam pakan terhadap pertumbuhan panjang dan bobot serta pematangan gonad ikan giru. Ikan giru diberi perlakuan dengan tiga dosis hormon Oodev berbeda yaitu 0 mL/kg, 0,5 mL/kg, dan 1 mL/kg dengan tiga pengulangan selama 30 hari pemeliharaan. Hasil menunjukkan dosis 1 mL/kg meningkatkan pertumbuhan panjang tubuh pada jantan (0,9 cm) maupun betina (0,7 cm) dan pertumbuhan bobot pada jantan (1,32 gram) maupun betina (3,05 gram) lebih tinggi dengan kontrol. Sejalan dengan pertumbuhan panjang dan bobot, dosis 1 mL/kg meningkatkan nilai gonadosomatic index jantan dan betina yaitu 0,47% dan 0,58% lebih tinggi dengan kontrol. Dari hasil yang diperoleh dapat ditarik kesimpulan bahwa hormon Oodev dengan dosis 1 mL/kg dapat meningkatkan pertumbuhan dan nilai GSI A. clarkii.