scholarly journals Recombinant aequorin and recombinant semi-synthetic aequorins. Cellular Ca2+ ion indicators

1990 ◽  
Vol 270 (2) ◽  
pp. 309-312 ◽  
Author(s):  
O Shimomura ◽  
S Inouye ◽  
B Musicki ◽  
Y Kishi
Keyword(s):  
Luminescence Intensity ◽  
Intensity Ratio ◽  
Synthetic Analogues ◽  
Chromatographic Behaviour ◽  
Recombinant Aequorin

Properties of a recombinant aequorin were investigated in comparison with those of natural aequorin. In chromatographic behaviour the recombinant aequorin did not match any of ten isoaequorins tested, although it was very similar to aequorin J. Its sensitivity to Ca2+ was found to be higher than that of any isoaequorin except aequorin D. The recombinant aequorin exhibited no toxicity when tested in various kinds of cells, even where samples of natural aequorin had been found to be toxic. Properties of four recombinant semi-synthetic aequorins (fch-, hcp-, e- and n-types), prepared from the recombinant apo-aequorin and synthetic analogues of coelenterazine, were approximately parallel with those of corresponding semi-synthetic aequorins prepared from natural apo-aequorin. Both recombinant e-aequorin and natural e-aequorin J luminesced with high values of the luminescence intensity ratio I400/I465, although the ratios were not pCa-dependent. The recombinant aequorin and recombinant semi-synthetic aequorins are highly suited for monitoring cellular Ca2+.

scholarly journals Semi-synthetic aequorins with improved sensitivity to Ca2+ ions

1989 ◽  
Vol 261 (3) ◽  
pp. 913-920 ◽  
Author(s):  
O Shimomura ◽  
B Musicki ◽  
Y Kishi
Keyword(s):  
Relative Intensity ◽  
Luminescence Intensity ◽  
Intensity Ratio ◽  
Ratio Method ◽  
Prominent Feature ◽  
Wide Range

Thirty-seven coelenterazine analogues were synthesized and incorporated into apo-aequorin, yielding 30 semi-synthetic aequorins that have the capacity to emit a significant amount of light in the presence of Ca2+. The properties of resultant photoproteins were investigated. The most prominent feature of those photoproteins was the wide range in their sensitivities to Ca2+ concentration. The relative intensity of Ca2+-triggered luminescence of the photoproteins ranged from 0.01 to 190 when compared with natural aequorin (relative intensity 1.0) at pCa 6 for the cases where the relative intensity is less than 1 and at pCa 7 for the cases where the relative intensity is higher than 1. Eight of the semi-synthetic aequorins belonged to the class of e-aequorin. With two of those photoproteins, the degree of dependence of the luminescence intensity ratio I400/I465 on pCa was greater than that with e-aequorin, suggesting that these two photoproteins are possibly superior to e-aequorin in measuring Ca2+ concentration by the ratio method.

Custom-built thermometry apparatus and luminescence intensity ratio thermometry of ZrO2:Eu3+ and Nb2O5:Eu3+

2019 ◽  
Vol 30 (4) ◽  
pp. 045001 ◽  
Author(s):  
Aleksandar Ćirić ◽  
Stevan Stojadinović ◽  
Miroslav D Dramićanin
Keyword(s):  
Luminescence Intensity ◽  
Intensity Ratio

Comparing the performance of Nd3+-doped LiBaPO4 phosphors as optical temperature sensors within the first biological window exploiting luminescence intensity ratio and bandwidth methods

2020 ◽  
Vol 227 ◽  
pp. 117524
Author(s):  
André Scheidegger Laia ◽  
Daniela A. Hora ◽  
Marcos V. dos S. Rezende ◽  
Yutao Xing ◽  
José Joatan Rodrigues ◽  
...  
Keyword(s):  
Luminescence Intensity ◽  
Intensity Ratio ◽  
Temperature Sensors

Size dependent sensitivity of Yb3+,Er3+ up-converting luminescent nano-thermometers

2017 ◽  
Vol 5 (31) ◽  
pp. 7890-7897 ◽  
Author(s):  
L. Marciniak ◽  
K. Prorok ◽  
A. Bednarkiewicz
Keyword(s):  
Luminescence Intensity ◽  
Electronic Transition ◽  
Intensity Ratio ◽  
Temperature Sensing ◽  
Contact Temperature ◽  
Size Dependent ◽  
Co Doped

The non-contact temperature sensing using the luminescence intensity ratio (LIR) between 2H11/2 → 4I15/2 and 4S3/2 → 4I15/2 electronic transition in upconverting Yb3+ and Er3+ co-doped nanocrystals has been known for two decades.

scholarly journals Luminescence Intensity Ratio Thermometry With Er3+: Performance Overview

2021 ◽  
Author(s):  
Aleksandar Ćirić ◽  
Tamara Gavrilović ◽  
Miroslav D. Dramićanin
Keyword(s):  
Luminescence Intensity ◽  
Intensity Ratio ◽  
Emission Spectra ◽  
Relative Sensitivity ◽  
Orbit Coupling ◽  
Slight Variation ◽  
Spin Orbit Coupling ◽  
Spin Orbit ◽  
Coupling Parameters ◽  
Better Than

The figures of merit of luminescence intensity ratio (LIR) thermometry for Er3+ in 40 different crystals and glasses have been calculated and compared. For calculations, the relevant data has been collected from the literature while the missing data were derived from available absorption and emission spectra. The calculated parameters include Judd-Ofelt parameters, refractive indexes, Slater integrals, spin-orbit coupling parameters, reduced matrix elements (RMEs), energy differences between emitting levels used for LIR, absolute and relative sensitivities. We found a slight variation of RMEs between hosts as a result of variations in values of Slater integrals and spin-orbit coupling parameters, and we calculated their average values over 40 hosts. The calculations showed that crystals perform better than glasses in Er3+ based thermometry, and we identified hosts that have large values of both absolute and relative sensitivity.

Luminescence Intensity Ratio thermometry and Judd-Ofelt analysis of TiO2:Eu3+

2018 ◽  
Vol 85 ◽  
pp. 261-266 ◽  
Author(s):  
Aleksandar Ćirić ◽  
Stevan Stojadinović ◽  
Miroslav D. Dramićanin
Keyword(s):  
Luminescence Intensity ◽  
Intensity Ratio

scholarly journals Up-Converting Luminescence and Temperature Sensing of Er3+/Tm3+/Yb3+ Co-Doped NaYF4 Phosphors Operating in Visible and the First Biological Window Range

Nanomaterials ◽  
2021 ◽  
Vol 11 (10) ◽  
pp. 2660
Author(s):  
Jingyun Li ◽  
Yuxiao Wang ◽  
Xueru Zhang ◽  
Liang Li ◽  
Haoyue Hao
Keyword(s):  
Energy Level ◽  
Luminescence Intensity ◽  
Industrial Production ◽  
Intensity Ratio ◽  
Temperature Sensors ◽  
Temperature Sensing ◽  
Optical Thermometry ◽  
Potential Applications ◽  
Sensing Sensitivity ◽  
Co Doped

Accurate and reliable non-contact temperature sensors are imperative for industrial production and scientific research. Here, Er3+/Tm3+/Yb3+ co-doped NaYF4 phosphors were studied as an optical thermometry material. The typical hydrothermal method was used to synthesize hexagonal Er3+/Tm3+/Yb3+ co-doped NaYF4 phosphors and the morphology was approximately rod-like. The up-conversion emissions of the samples were located at 475, 520, 550, 650, 692 and 800 nm. Thermo-responsive emissions from the samples were monitored to evaluate the relative sensing sensitivity. The thermal coupled energy level- and non-thermal coupled energy level-based luminescence intensity ratio thermometry of the sample demonstrated that these two methods can be used to test temperature. Two green emissions (520 and 550 nm), radiated from 2H11/2/4S3/2 levels, were monitored, and the maximum relative sensing sensitivities reached to 0.013 K−1 at 297 K. The emissions located in the first biological window (650, 692 and 800 nm) were monitored and the maximum relative sensing sensitivities reached to 0.027 (R692/650) and 0.028 K−1 (R692/800) at 297 K, respectively. These results indicate that Er3+/Tm3+/Yb3+ co-doped NaYF4 phosphors have potential applications for temperature determination in the visible and the first biological window ranges.

scholarly journals Luminescence Intensity Ratio Thermometry with Er3+: Performance Overview

Crystals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 189
Author(s):  
Aleksandar Ćirić ◽  
Tamara Gavrilović ◽  
Miroslav D. Dramićanin
Keyword(s):  
Luminescence Intensity ◽  
Intensity Ratio ◽  
Emission Spectra ◽  
Relative Sensitivity ◽  
Orbit Coupling ◽  
Slight Variation ◽  
Spin Orbit Coupling ◽  
Spin Orbit ◽  
Coupling Parameters ◽  
Better Than

The figures of merit of luminescence intensity ratio (LIR) thermometry for Er3+ in 40 different crystals and glasses have been calculated and compared. For calculations, the relevant data has been collected from the literature while the missing data were derived from available absorption and emission spectra. The calculated parameters include Judd–Ofelt parameters, refractive indexes, Slater integrals, spin–orbit coupling parameters, reduced matrix elements (RMEs), energy differences between emitting levels used for LIR, absolute, and relative sensitivities. We found a slight variation of RMEs between hosts because of variations in values of Slater integrals and spin–orbit coupling parameters, and we calculated their average values over 40 hosts. The calculations showed that crystals perform better than glasses in Er3+-based thermometry, and we identified hosts that have large values of both absolute and relative sensitivity.

A two-target responsive reversible ratiometric pH nanoprobe: a white light emitting quantum dot complex

2019 ◽  
Vol 55 (30) ◽  
pp. 4331-4334 ◽  
Author(s):  
Sabyasachi Pramanik ◽  
Shilaj Roy ◽  
Arup Mondal ◽  
Satyapriya Bhandari
Keyword(s):  
Quantum Dot ◽  
White Light ◽  
Luminescence Intensity ◽  
Intensity Ratio ◽  
Ph Sensing ◽  
Light Emitting ◽  
Physiological Range ◽  
Ratiometric Ph Sensing

Ratiometric pH sensing in the physiological range of pH 6.5–10.3 by a white light emitting quantum dot complex – following the changes in luminescence intensity ratio, color and chromaticity – is described herein.

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