Identification by protein microsequencing of a proteinase-V8-cleavage site in a folding intermediate of chick muscle creatine kinase
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We have identified by protein microsequencing a glutamic acid residue (Glu-166) in a folding intermediate of chick muscle creatine kinase that is very sensitive to cleavage by staphylococcal proteinase V8. Most other glutamic acid residues, including Glu-168, are already partly protected from proteolytic attack at this stage. After the final stages of protein refolding, when enzyme activity is recovered, Glu-166 is also resistant to proteolysis.
1984 ◽
Vol 259
(24)
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pp. 15224-15227
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1994 ◽
Vol 269
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pp. 16740-16745
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1989 ◽
Vol 125
(1-2)
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pp. 251-259
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1990 ◽
Vol 10
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pp. 4826-4836
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2004 ◽
Vol 31
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pp. 43-50
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