scholarly journals Localization of xanthine dehydrogenase mRNA in horse skeletal muscle by in situ hybridization with digoxigenin-labelled probe

1993 ◽  
Vol 292 (3) ◽  
pp. 639-641 ◽  
Author(s):  
L A Räsänen ◽  
U Karvonen ◽  
A R Pösö
Keyword(s):  
Skeletal Muscle ◽  
Endothelial Cells ◽  
In Situ Hybridization ◽  
Northern Blot ◽  
Cdna Sequence ◽  
Muscle Cells ◽  
Xanthine Dehydrogenase ◽  
Capillary Endothelial Cells

In situ hybridization was used to localize xanthine dehydrogenase (XDH) mRNA in horse skeletal muscle. Capillary endothelial cells were found to express XDH, but muscle cells did not give any signal. The digoxigenin-labelled probe was produced by PCR with primers based on the cDNA sequence of mouse XDH and horse lung cDNAs. A 4.3 kb mRNA was detected in a Northern blot.

scholarly journals Localization of erythropoietin synthesizing cells in murine kidneys by in situ hybridization

Blood ◽  
1988 ◽  
Vol 71 (2) ◽  
pp. 524-527 ◽  
Author(s):  
ST Koury ◽  
MC Bondurant ◽  
MJ Koury
Keyword(s):  
Endothelial Cells ◽  
In Situ Hybridization ◽  
Basement Membrane ◽  
Interstitial Cells ◽  
Tubular Basement Membrane ◽  
Paraffin Embedding ◽  
Capillary Endothelial Cells ◽  
Rna Probe ◽  
Labeled Rna

Abstract In situ hybridization was used to localize the cells that produce erythropoietin (EP) in anemic murine kidneys. Kidneys from anemic and nonanemic mice were fixed and processed for paraffin embedding. Sections were hybridized with a 35S-labeled RNA probe complementary to mRNA coding for EP. An uncommon, but specific type of cell was intensely labeled in the cortices of anemic kidneys. The labeled cells were clearly nonglomerular and nontubular. Their location outside of the tubular basement membrane was consistent with that of a subset of interstitial cells or capillary endothelial cells.

scholarly journals Localization of erythropoietin synthesizing cells in murine kidneys by in situ hybridization

Blood ◽  
1988 ◽  
Vol 71 (2) ◽  
pp. 524-527 ◽  
Author(s):  
ST Koury ◽  
MC Bondurant ◽  
MJ Koury
Keyword(s):  
Endothelial Cells ◽  
In Situ Hybridization ◽  
Basement Membrane ◽  
Interstitial Cells ◽  
Tubular Basement Membrane ◽  
Paraffin Embedding ◽  
Capillary Endothelial Cells ◽  
Rna Probe ◽  
Labeled Rna

In situ hybridization was used to localize the cells that produce erythropoietin (EP) in anemic murine kidneys. Kidneys from anemic and nonanemic mice were fixed and processed for paraffin embedding. Sections were hybridized with a 35S-labeled RNA probe complementary to mRNA coding for EP. An uncommon, but specific type of cell was intensely labeled in the cortices of anemic kidneys. The labeled cells were clearly nonglomerular and nontubular. Their location outside of the tubular basement membrane was consistent with that of a subset of interstitial cells or capillary endothelial cells.

Developmental changes in the expression of growth-associated protein-43 mRNA in the monkey thalamus: Northern blot and in situ hybridization studies

Neuroscience ◽  
2005 ◽  
Vol 136 (2) ◽  
pp. 497-507 ◽  
Author(s):  
Y. Murata ◽  
N. Higo ◽  
T. Oishi ◽  
A. Yamashita ◽  
K. Matsuda ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Northern Blot ◽  
Developmental Changes

Expression of alpha 1 integrin, a laminin-collagen receptor, during myogenesis and neurogenesis in the avian embryo

Development ◽  
1992 ◽  
Vol 116 (3) ◽  
pp. 585-600 ◽  
Author(s):  
J.L. Duband ◽  
A.M. Belkin ◽  
J. Syfrig ◽  
J.P. Thiery ◽  
V.E. Koteliansky
Keyword(s):  
Skeletal Muscle ◽  
Endothelial Cells ◽  
Smooth Muscle ◽  
Smooth Muscles ◽  
Collagen Iv ◽  
Integrin Subunit ◽  
Avian Embryo ◽  
Subunit Expression ◽  
Capillary Endothelial Cells ◽  
Collagen Receptor

In this study, we have examined the spatiotemporal distribution of the alpha 1 integrin subunit, a putative laminin and collagen receptor, in avian embryos, using immunofluorescence microscopy and immunoblotting techniques. We used an antibody raised against a gizzard 175 × 10(3) M(r) membrane protein which was described previously and which we found to be immunologically identical to the chicken alpha 1 integrin subunit. In adult avian tissues, alpha 1 integrin exhibited a very restricted pattern of expression; it was detected only in smooth muscle and in capillary endothelial cells. In the developing embryo, alpha 1 integrin subunit expression was discovered in addition to smooth muscle and capillary endothelial cells, transiently, in both central and peripheral nervous systems and in striated muscles, in association with laminin and collagen IV. alpha 1 integrin was practically absent from most epithelial tissues, including the liver, pancreas and kidney tubules, and was weakly expressed by tissues that were not associated with laminin and collagen IV. In the nervous system, alpha 1 integrin subunit expression occurred predominantly at the time of early neuronal differentiation. During skeletal muscle development, alpha 1 integrin was expressed on myogenic precursors, during myoblast migration, and in differentiating myotubes. alpha 1 integrin disappeared from skeletal muscle cells as they became contractile. In visceral and vascular smooth muscles, alpha 1 integrin appeared specifically during early smooth muscle cell differentiation and, later, was permanently expressed after cell maturation. These results indicate that (i) the expression pattern of alpha 1 integrin is consistent with a function as a laminin/collagen IV receptor; (ii) during avian development, expression of the alpha 1 integrin subunit is spatially and temporally regulated; (iii) during myogenesis and neurogenesis, expression of alpha 1 integrin is transient and correlates with cell migration and differentiation.

Cloning and widespread distribution of the rat rod-type cyclic nucleotide-gated cation channel

1997 ◽  
Vol 272 (4) ◽  
pp. C1335-C1344 ◽  
Author(s):  
C. Ding ◽  
E. D. Potter ◽  
W. Qiu ◽  
S. L. Coon ◽  
M. A. Levine ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
In Situ Hybridization ◽  
Pineal Gland ◽  
Northern Blot ◽  
Blot Analysis ◽  
Cyclic Nucleotide ◽  
Northern Blot Analysis ◽  
Chain Reaction ◽  
Polymerase Chain

We used Northern blot analysis, ribonuclease protection assay (RPA), reverse transcriptase-polymerase chain reaction, and in situ hybridization to investigate the hypothesis that the CNG1 isoform of the cyclic nucleotide-gated nonselective cation channel may be widely distributed in tissues of the rat. A cDNA encoding the CNG1 isoform was isolated from rat eye and human retina, and partial sequences were isolated from rat pineal gland and human kidney. Northern blot analysis revealed a 3.1-kilobase (kb) CNG1 transcript in rat eye, pineal gland, pituitary, adrenal gland, and spleen, and a larger transcript of 3.5 kb was found in testis. RPA confirmed the identity of CNG1 mRNA in rat eye, lung, spleen, and brain. Polymerase chain reaction-based detection of the mRNA for CNG1 indicates that the channel is expressed in lower abundance in many other tissues, including thymus, skeletal muscle, heart, and parathyroid gland. The cellular distribution of CNG1 was further studied by in situ hybridization, which demonstrated expression of mRNA in lung, thymus, pineal gland, hippocampus, cerebellum, and cerebral cortex but not in heart or kidney.

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