scholarly journals Biotin binders selected from a random peptide library expressed on phage

1993 ◽  
Vol 293 (3) ◽  
pp. 613-616 ◽  
Author(s):  
I Saggio ◽  
R Laufer
Keyword(s):  
Synthetic Peptide ◽  
Peptide Library ◽  
Binding Motif ◽  
Sequence Motif ◽  
Phage Display Technology ◽  
Random Peptide Library ◽  
Random Peptide ◽  
Biotin Binding ◽  
Display Technology ◽  
Streptavidin Binding

Recombinant biotin-binding phages were affinity-selected from a random peptide library expressed on the surface of filamentous phage. Phage binding to biotinylated proteins was half-maximally inhibited by micromolar concentrations of a monobiotinylated molecule. Sequencing of the peptide inserts of selected phages led to the identification of a previously unknown biotin-binding motif, CXWXPPF(K or R)XXC. A synthetic peptide containing this sequence motif inhibited streptavidin binding to biotinylated BSA with an IC50 of 50 microM. This compound represents the shortest non-avidin biotin-binding peptide identified to date. Our results illustrate that phage display technology can be used to identify novel ligands for a small non-proteinaceous molecule.

scholarly journals Epitope Fingerprinting for Recognition of the Polyclonal Serum Autoantibodies of Alzheimer’s Disease

2015 ◽  
Vol 2015 ◽  
pp. 1-8 ◽  
Author(s):  
Luiz Carlos de Oliveira-Júnior ◽  
Fabiana de Almeida Araújo Santos ◽  
Luiz Ricardo Goulart ◽  
Carlos Ueira-Vieira
Keyword(s):  
Alzheimer’S Disease ◽  
Alzheimer's Disease ◽  
Phage Display ◽  
Neural Tissue ◽  
Peptide Library ◽  
Phage Display Technology ◽  
Random Peptide Library ◽  
Random Peptide ◽  
Display Technology ◽  
Polyclonal Serum

Autoantibodies (aAb) associated with Alzheimer’s disease (AD) have not been sufficiently characterized and their exact involvement is undefined. The use of information technology and computerized analysis with phage display technology was used, in the present research, to map the epitope of putative self-antigens in AD patients. A 12-mer random peptide library, displayed on M13 phages, was screened using IgG from AD patients with two repetitions. Seventy-one peptides were isolated; however, only 10 were positive using the Elisa assay technique (Elisa Index > 1). The results showed that the epitope regions of the immunoreactive peptides, identified by phage display analysis, were on the exposed surfaces of the proteins. The putative antigens MAST1, Enah, MAO-A, X11/MINT1, HGF, SNX14, ARHGAP 11A, APC, and CENTG3, which have been associated with AD or have functions in neural tissue, may indicate possible therapeutic targets.

36. Random Peptide Library Based on a Spider Neurotoxin, and Utilization of the Library in in vitro Evolution Directed to GPCR Ligands

Toxicon ◽  
2012 ◽  
Vol 60 (2) ◽  
pp. 113 ◽  
Author(s):  
Tai Kubo ◽  
Seigo Ono ◽  
Tadashi Kimura ◽  
Suzuko Kobayashi ◽  
Tetsuro Kondo ◽  
...  
Keyword(s):  
Peptide Library ◽  
In Vitro Evolution ◽  
Random Peptide Library ◽  
Random Peptide

Definition of the peptide mimotope of cellular receptor for hepatitis C virus E2 protein using random peptide library

2001 ◽  
Vol 1 (1) ◽  
pp. 77
Author(s):  
In-Hee Lee ◽  
Jae-Eun Paik ◽  
Sang-Yong Seol ◽  
Dae-Hyun Seog ◽  
Sae-Gwang Park ◽  
...  
Keyword(s):  
Hepatitis C Virus ◽  
Hepatitis C ◽  
Peptide Library ◽  
Cellular Receptor ◽  
E2 Protein ◽  
Random Peptide Library ◽  
Random Peptide ◽  
Definition Of

Isolation of lung tumor specific peptides from a random peptide library: generation of diagnostic and cell-targeting reagents

2003 ◽  
Vol 202 (2) ◽  
pp. 219-230 ◽  
Author(s):  
Tsuksa Oyama ◽  
Kathryn F. Sykes ◽  
Kausar N. Samli ◽  
John D. Minna ◽  
Stephen Albert Johnston ◽  
...  
Keyword(s):  
Lung Tumor ◽  
Peptide Library ◽  
Cell Targeting ◽  
Random Peptide Library ◽  
Random Peptide

scholarly journals Identification of Peptides That Inhibit the DNA Binding, trans-Activator, and DNA Replication Functions of the Human Papillomavirus Type 11 E2 Protein

2004 ◽  
Vol 78 (5) ◽  
pp. 2637-2641 ◽  
Author(s):  
Su-Jun Deng ◽  
Kenneth H. Pearce ◽  
Eric P. Dixon ◽  
Kelly A. Hartley ◽  
Thomas B. Stanley ◽  
...  
Keyword(s):  
Cell Culture ◽  
Human Papillomavirus ◽  
Dna Replication ◽  
Transcriptional Activation ◽  
Synthetic Peptides ◽  
Dna Interaction ◽  
Peptide Library ◽  
Random Peptide Library ◽  
Random Peptide

ABSTRACT Peptide antagonists of the human papillomavirus type 11 (HPV-11) E2-DNA association were identified using a filamentous bacteriophage random peptide library. Synthetic peptides antagonized the E2-DNA interaction, effectively blocked E2-mediated transcriptional activation of a reporter gene in cell culture, and inhibited E1-E2-mediated HPV-11 DNA replication in vitro. These peptides may prove to be useful tools for characterizing E2 function and for exploring the effectiveness of E2-inhibitor-based treatments for HPV-associated diseases.

Mimotope of Leptospira from phage-displayed random peptide library is reactive with both monoclonal antibodies and patients’ sera

2006 ◽  
Vol 115 (1-3) ◽  
pp. 54-63 ◽  
Author(s):  
R TUNGTRAKANPOUNG ◽  
P PITAKSAJJAKUL ◽  
N NANGARM ◽  
W CHAICUMPA ◽  
P EKPO ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Peptide Library ◽  
Random Peptide Library ◽  
Random Peptide
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