Post-Mortem High-Energy Phosphate and Glycolytic Changes in Two Skeletal Muscles of the Ox

1977 ◽  
Vol 5 (6) ◽  
pp. 1741-1744 ◽  
Author(s):  
CARMEL MOTHERSILL ◽  
J. V. McLOUGHLIN
Keyword(s):  
Skeletal Muscles ◽  
High Energy ◽  
Post Mortem ◽  
High Energy Phosphate

scholarly journals A temporal dissociation of energy liberation and high energy phosphate splitting during shortening in frog skeletal muscles.

1976 ◽  
Vol 68 (1) ◽  
pp. 13-27 ◽  
Author(s):  
J A Rall ◽  
E Homsher ◽  
A Wallner ◽  
W F Mommaerts
Keyword(s):  
Stimulus Pattern ◽  
Skeletal Muscles ◽  
Energy Production ◽  
Time Course ◽  
Rana Pipiens ◽  
High Energy ◽  
Energy Liberation ◽  
High Energy Phosphate ◽  
Contracting Muscle ◽  
Mechanical Measurements

Measurements of the time course of high energy phosphate splitting and energy liberation were performed on rapidly shortening Rana pipiens skeletal muscles. In muscles contracting 30 times against small loads (less the 0.02P), the ratio of explained heat + work (H + W) (calculated from the measured high energy phosphate splitting) to observed H + W (from myothermal and mechanical measurements) was 0.68 +/- 0.08 and is in agreement with results obtained in isometric tetani of R. pipiens skeletal muscle. In lightly afterloaded muscles which were tetanized for 0.6a and whose metabolism was arrested at 3.0 s after the beginning of stimulation, a similar ratio of explained H + W to observed H + W was obtained. However, in identical contractions in which metabolism was arrested at 0.5-0.75 s after the beginning of stimulation, the ratio of explained H + W to observed H + W declined significantly to values ranging from 0.15 to 0.40. These results suggest that rapid shortening at the beginning of contraction induces a delay between energy production and measurable high energy phosphate splitting. This interpretation was tested and confirmed in experiments in which one muscle of a pair contracted isometrically while the other contracted against a small afterload. The afterload and stimulus pattern were arranged so that at the time metabolism was arrested, 0.5 s after the beginning of stimulation, the total energy production by both muscles was the same. Chemical analysis revealed that the isotonically contracting muscle spilt only 25% as much high energy phosphate as did the isometrically contracting muscle.

scholarly journals Energy balance studies in frog skeletal muscles shortening at one-half maximal velocity.

1984 ◽  
Vol 84 (3) ◽  
pp. 347-359 ◽  
Author(s):  
E Homsher ◽  
T Yamada ◽  
A Wallner ◽  
J Tsai
Keyword(s):  
Skeletal Muscles ◽  
Maximum Velocity ◽  
High Energy ◽  
Sartorius Muscle ◽  
Maximal Velocity ◽  
High Energy Phosphate ◽  
The Mean ◽  
S Period ◽  
High Energy Phosphate Metabolism ◽  
Frog Sartorius

High-energy phosphate metabolism and energy liberated as heat and work were measured in 3-s tetani of frog sartorius muscle at 0 degree C. Two contraction periods were studied: (a) a 0.35-s period of shortening near half-maximum velocity beginning after 2 s of isometric stimulation, and (b) a 0.65-s isometric period immediately following the shortening. There were no significant changes in levels of ATP, ADP, or AMP in the two contraction periods. The observed changes in inorganic phosphate and creatine levels indicated that the only significant reaction occurring was phosphocreatine splitting. The mean rate of high-energy phosphate splitting during the shortening, 1.60 +/- 0.23 mumol X g-1 X s-1 (n = 24), was about fivefold higher than that in the 1-s period in the isometric tetanus, 0.32 +/- 0.11 mumol X g-1 X s-1 (n = 17), observed in our previous study. The mean rate in the post-shortening period, 0.46 +/- 0.13 mumol X g-1 X s-1 (n = 17), was not significantly different from that in the 1-s period in the isometric tetanus. A large amount of heat plus work was produced during the shortening period, and this could be accounted for by simultaneous chemical changes. In the post-shortening period, the observed enthalpy was also accounted for by simultaneous chemical reactions. Thus, the present result is in sharp contrast to that obtained from a similar study performed at a shortening at Vmax, where an enthalpy excess was produced during shortening and an enthalpy deficit was produced during the period following the shortening.

Interleaved MRI/MRS study of muscle perfusion, oxygenation and high energy phosphate metabolism in normal subjects and Becker's myopathic patients

1998 ◽  
Vol 95 (2) ◽  
pp. 250-255
Author(s):  
J. F. Toussaint ◽  
C. Brillault-Salvat ◽  
E. Giacomini ◽  
G. Bloch ◽  
D. Duboc ◽  
...  
Keyword(s):  
Normal Subjects ◽  
High Energy ◽  
Phosphate Metabolism ◽  
High Energy Phosphate ◽  
Muscle Perfusion ◽  
High Energy Phosphate Metabolism

scholarly journals The “High Energy Phosphate Bond” Concept

1960 ◽  
Vol 10 ◽  
pp. 1-53 ◽  
Author(s):  
Philip George ◽  
Robert J. Rutman
Keyword(s):  
High Energy ◽  
High Energy Phosphate ◽  
Phosphate Bond

scholarly journals Energy Metabolism of Human Leukemic Lymphocytes and Granulocytes

Blood ◽  
1967 ◽  
Vol 30 (2) ◽  
pp. 151-167 ◽  
Author(s):  
JOHN LASZLO ◽  
Clarence Ellis
Keyword(s):  
Energy Metabolism ◽  
Chronic Lymphocytic Leukemia ◽  
Glucose Concentration ◽  
Acute Lymphocytic Leukemia ◽  
Aerobic Glycolysis ◽  
High Energy ◽  
Lymphocytic Leukemia ◽  
Anaerobic Conditions ◽  
High Energy Phosphate

Abstract 1. Leukocytes taken from patients having acute lymphocytic leukemia and chronic lymphocytic leukemia are characterized by high respiratory rates and low to absent aerobic glycolysis. Leukemic granulocytes have low respiratory rates and high aerobic glycolysis. 2. Lymphocytes and granulocytes have the capacity for high glycolytic rates under anaerobic conditions. 3. Lymphocyte respiration is independent of glucose concentration in contrast to granulocyte respiration. 4. High energy phosphate levels of lymphocytes and granulocytes are unchanged if these cells are incubated aerobically, either with or without glucose, or anaerobically in the presence of glucose. 5. Aerobic glycolysis can be induced in lymphocytes by the addition of foreign plasma. Foreign plasma may also alter granulocyte metabolism.

Responses of beta-adrenoceptor in rat soleus to phosphorus compound levels and/or unloading

1994 ◽  
Vol 266 (5) ◽  
pp. C1257-C1262 ◽  
Author(s):  
Y. Ohira ◽  
K. Saito ◽  
T. Wakatsuki ◽  
W. Yasui ◽  
T. Suetsugu ◽  
...  
Keyword(s):  
Contractile Activity ◽  
Creatine Supplementation ◽  
High Energy ◽  
Hindlimb Suspension ◽  
Phosphorus Compounds ◽  
High Energy Phosphates ◽  
High Energy Phosphate ◽  
Gravitational Unloading ◽  
Beta Adrenoceptor ◽  
Muscle Contractile

Responses of beta-adrenoceptor (beta-AR) in rat soleus to gravitational unloading and/or changes in the levels of phosphorus compounds by feeding either creatine or its analogue beta-guanidinopropionic acid (beta-GPA) were studied. A decrease in the density of beta-AR (about -35%) was induced by 10 days of hindlimb suspension, but the affinity of the receptor was unaffected. Suspension unloading tended to increase the levels of adenosine triphosphate and phosphocreatine and decrease inorganic phosphate. Even without unloading, the beta-AR density decreased after an oral creatine supplementation (about -20%), which also tended to elevate the high-energy phosphate levels in muscle. However, an elevation of beta-AR density was induced (about +36%) after chronic depletion of high-energy phosphates by feeding beta-GPA (about +125%). Data suggest that the density of beta-AR in muscle is elevated if the high-energy phosphate contents are chronically decreased and vice versa. However, it may not be directly related to the degree of muscle contractile activity.

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