scholarly journals Energy balance studies in frog skeletal muscles shortening at one-half maximal velocity.

1984 ◽  
Vol 84 (3) ◽  
pp. 347-359 ◽  
Author(s):  
E Homsher ◽  
T Yamada ◽  
A Wallner ◽  
J Tsai
Keyword(s):  
Skeletal Muscles ◽  
Maximum Velocity ◽  
High Energy ◽  
Sartorius Muscle ◽  
Maximal Velocity ◽  
High Energy Phosphate ◽  
The Mean ◽  
S Period ◽  
High Energy Phosphate Metabolism ◽  
Frog Sartorius

High-energy phosphate metabolism and energy liberated as heat and work were measured in 3-s tetani of frog sartorius muscle at 0 degree C. Two contraction periods were studied: (a) a 0.35-s period of shortening near half-maximum velocity beginning after 2 s of isometric stimulation, and (b) a 0.65-s isometric period immediately following the shortening. There were no significant changes in levels of ATP, ADP, or AMP in the two contraction periods. The observed changes in inorganic phosphate and creatine levels indicated that the only significant reaction occurring was phosphocreatine splitting. The mean rate of high-energy phosphate splitting during the shortening, 1.60 +/- 0.23 mumol X g-1 X s-1 (n = 24), was about fivefold higher than that in the 1-s period in the isometric tetanus, 0.32 +/- 0.11 mumol X g-1 X s-1 (n = 17), observed in our previous study. The mean rate in the post-shortening period, 0.46 +/- 0.13 mumol X g-1 X s-1 (n = 17), was not significantly different from that in the 1-s period in the isometric tetanus. A large amount of heat plus work was produced during the shortening period, and this could be accounted for by simultaneous chemical changes. In the post-shortening period, the observed enthalpy was also accounted for by simultaneous chemical reactions. Thus, the present result is in sharp contrast to that obtained from a similar study performed at a shortening at Vmax, where an enthalpy excess was produced during shortening and an enthalpy deficit was produced during the period following the shortening.

Differential energetic response of brain vs. skeletal muscle upon glycemic variations in healthy humans

2008 ◽  
Vol 294 (1) ◽  
pp. R12-R16 ◽  
Author(s):  
Kerstin M. Oltmanns ◽  
Uwe H. Melchert ◽  
Harald G. Scholand-Engler ◽  
Maria C. Howitz ◽  
Bernd Schultes ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Underlying Mechanism ◽  
High Energy ◽  
Resonance Spectroscopy ◽  
Energy Status ◽  
High Energy Phosphate ◽  
Available Energy ◽  
Healthy Humans ◽  
High Energy Phosphate Metabolism ◽  
The Brain

The brain regulates all metabolic processes within the organism, and therefore, its energy supply is preserved even during fasting. However, the underlying mechanism is unknown. Here, it is shown, using 31P-magnetic resonance spectroscopy that during short periods of hypoglycemia and hyperglycemia, the brain can rapidly increase its high-energy phosphate content, whereas there is no change in skeletal muscle. We investigated the key metabolites of high-energy phosphate metabolism as rapidly available energy stores by 31P MRS in brain and skeletal muscle of 17 healthy men. Measurements were performed at baseline and during dextrose or insulin-induced hyperglycemia and hypoglycemia. During hyperglycemia, phosphocreatine (PCr) concentrations increased significantly in the brain ( P = 0.013), while there was a similar trend in the hypopglycemic condition ( P = 0.055). Skeletal muscle content remained constant in both conditions ( P > 0.1). ANOVA analyses comparing changes from baseline to the respective glycemic plateau in brain (up to +15%) vs. muscle (up to −4%) revealed clear divergent effects in both conditions ( P < 0.05). These effects were reflected by PCr/Pi ratio ( P < 0.05). Total ATP concentrations revealed the observed divergency only during hyperglycemia ( P = 0.018). These data suggest that the brain, in contrast to peripheral organs, can activate some specific mechanisms to modulate its energy status during variations in glucose supply. A disturbance of these mechanisms may have far-reaching implications for metabolic dysregulation associated with obesity or diabetes mellitus.

scholarly journals A temporal dissociation of energy liberation and high energy phosphate splitting during shortening in frog skeletal muscles.

1976 ◽  
Vol 68 (1) ◽  
pp. 13-27 ◽  
Author(s):  
J A Rall ◽  
E Homsher ◽  
A Wallner ◽  
W F Mommaerts
Keyword(s):  
Stimulus Pattern ◽  
Skeletal Muscles ◽  
Energy Production ◽  
Time Course ◽  
Rana Pipiens ◽  
High Energy ◽  
Energy Liberation ◽  
High Energy Phosphate ◽  
Contracting Muscle ◽  
Mechanical Measurements

Measurements of the time course of high energy phosphate splitting and energy liberation were performed on rapidly shortening Rana pipiens skeletal muscles. In muscles contracting 30 times against small loads (less the 0.02P), the ratio of explained heat + work (H + W) (calculated from the measured high energy phosphate splitting) to observed H + W (from myothermal and mechanical measurements) was 0.68 +/- 0.08 and is in agreement with results obtained in isometric tetani of R. pipiens skeletal muscle. In lightly afterloaded muscles which were tetanized for 0.6a and whose metabolism was arrested at 3.0 s after the beginning of stimulation, a similar ratio of explained H + W to observed H + W was obtained. However, in identical contractions in which metabolism was arrested at 0.5-0.75 s after the beginning of stimulation, the ratio of explained H + W to observed H + W declined significantly to values ranging from 0.15 to 0.40. These results suggest that rapid shortening at the beginning of contraction induces a delay between energy production and measurable high energy phosphate splitting. This interpretation was tested and confirmed in experiments in which one muscle of a pair contracted isometrically while the other contracted against a small afterload. The afterload and stimulus pattern were arranged so that at the time metabolism was arrested, 0.5 s after the beginning of stimulation, the total energy production by both muscles was the same. Chemical analysis revealed that the isotonically contracting muscle spilt only 25% as much high energy phosphate as did the isometrically contracting muscle.

Action of acetylcholine, choline and D-tubocurarine on the membrane of frog sartorius muscle fibers

1959 ◽  
Vol 196 (6) ◽  
pp. 1191-1196 ◽  
Author(s):  
S. Ochs ◽  
A. K. Mukherjee
Keyword(s):  
Membrane Potential ◽  
Mechanical Stimulation ◽  
Muscle Fibers ◽  
Potential Drop ◽  
Sartorius Muscle ◽  
End Plate ◽  
Frog Sartorius Muscle ◽  
Before And After ◽  
The Mean ◽  
Frog Sartorius

Resting potentials of frog sartorius muscle fibers were taken with microelectrodes at different distances along the length of muscles before and after adding acetylcholine or choline. The mean membrane potential drop and scatter of the potentials recorded in the relatively nerve-free, and in the more densely innervated parts of the muscle, were similar. The loss of direct excitability to electrical and mechanical stimulation was correlated with the concentration of choline or acetylcholine presented. d-tubocurarine added beforehand protected against the depolarizing effect of acetylcholine and choline everywhere along the length of the muscle. A generalized action of acetylcholine and choline and also of d-tubocurarine all along the muscle fibers was inferred. This generalized action at higher concentrations of acetylcholine and choline is believed to be additional to a more specific end plate action.

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