Mammalian Cell Culture: The Use of Serum-Free Hormone-Supplemented Media

1985 ◽  
Vol 13 (5) ◽  
pp. 974-975
Author(s):  
D. BLOXHAM
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Mammalian Cell Culture ◽  
Serum Free

scholarly journals Evaluation of recombinant human transferrin (DeltaFerrinTM) as an iron chelator in serum-free media for mammalian cell culture

2006 ◽  
Vol 51 (1) ◽  
pp. 29-37 ◽  
Author(s):  
Joanne Keenan ◽  
Dermot Pearson ◽  
Lorraine O’Driscoll ◽  
Patrick Gammell ◽  
Martin Clynes
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Mammalian Cell Culture ◽  
Iron Chelator ◽  
Serum Free ◽  
Human Transferrin ◽  
Free Media

Harvesting and concentration of human influenza A virus produced in serum-free mammalian cell culture for the production of vaccines

2007 ◽  
Vol 97 (1) ◽  
pp. 73-85 ◽  
Author(s):  
Bernd Kalbfuss ◽  
Yvonne Genzel ◽  
Michael Wolff ◽  
Anke Zimmermann ◽  
Robert Morenweiser ◽  
...  
Keyword(s):  
Cell Culture ◽  
Influenza A Virus ◽  
Mammalian Cell ◽  
Influenza A ◽  
Mammalian Cell Culture ◽  
Human Influenza ◽  
Serum Free

DEVELOPMENT OF SERUM-FREE MEDIA FOR MAMMALIAN CELL CULTURE

1991 ◽  
pp. 133-136 ◽  
Author(s):  
J. Kierulff ◽  
B. Persson ◽  
P. Rexen ◽  
C. Morcel ◽  
C. Emborg
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Mammalian Cell Culture ◽  
Serum Free ◽  
Free Media

Miniature Bioreactors for Rapid Bioprocess Development of Mammalian Cell Culture

2012 ◽  
Vol 59 (1) ◽  
Author(s):  
Mohd Helmi Sani ◽  
Frank Baganz
Keyword(s):  
Cell Culture ◽  
Mammalian Cell ◽  
Process Development ◽  
Mammalian Cell Culture ◽  
Small Scale ◽  
Cell Culture Process ◽  
Deep Wells ◽  
Working Volume ◽  
Culture Process ◽  
And Control

At present, there are a number of commercial small scale shaken systems available on the market with instrumented controllable microbioreactors such as Micro–24 Microreactor System (Pall Corporation, Port Washington, NY) and M2P Biolector, (M2P Labs GmbH, Aachen, Germany). The Micro–24 system is basically an orbital shaken 24–well plate that operates at working volume 3 – 7 mL with 24 independent reactors (deep wells, shaken and sparged) running simultaneously. Each reactor is designed as single use reactor that has the ability to continuously monitor and control the pH, DO and temperature. The reactor aeration is supplied by sparging air from gas feeds that can be controlled individually. Furthermore, pH can be controlled by gas sparging using either dilute ammonia or carbon dioxide directly into the culture medium through a membrane at the bottom of each reactor. Chen et al., (2009) evaluated the Micro–24 system for the mammalian cell culture process development and found the Micro–24 system is suitable as scaledown tool for cell culture application. The result showed that intra-well reproducibility, cell growth, metabolites profiles and protein titres were scalable with 2 L bioreactors.

Adaptation of an insect cell line (Agallia constricta) in a mammalian cell culture medium

In Vitro ◽  
1973 ◽  
Vol 8 (5) ◽  
pp. 375-378 ◽  
Author(s):  
Arthur H. Intosh ◽  
K. Maramorosch ◽  
C. Rechtoris
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Mammalian Cell ◽  
Insect Cell ◽  
Culture Medium ◽  
Mammalian Cell Culture ◽  
Insect Cell Line ◽  
Cell Culture Medium
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