Abnormalities in the Sodium Pump of Erythrocytes from Patients with Hyperthyroidism

1970 ◽  
Vol 38 (1) ◽  
pp. 49-61 ◽  
Author(s):  
E. K. M. Smith ◽  
Pamela D. Samuel
Keyword(s):  
Sodium Pump ◽  
Sodium Concentration ◽  
Intracellular Sodium ◽  
Red Cells ◽  
Control Group ◽  
Active Sodium ◽  
Sodium Efflux ◽  
Control Subjects ◽  
Intracellular Sodium Concentration ◽  
The Mean

1. Intracellular cation composition has been measured in the red cells from twenty patients with hyperthyroidism. The mean concentration of sodium was 11·18 m-mole/l red cells; in sixty normal control subjects the mean red cell sodium level was 7·04 m-mole/l. The difference between these two groups was highly significant. There was no measurable difference between the potassium concentration and water content of red cells from thyrotoxic and control groups. 2. Measurements of active sodium efflux were carried out in red cells from ten hyperthyroid subjects and their matched controls. The rate constant for active sodium efflux was significantly lower in the patients than the control group. 3. The total amount of sodium actively pumped from red cells in 1 hr was significantly higher in the patients than the controls. 4. The total amount of sodium moving out of the red cells, both actively and by exchange diffusion, matched the total influx of sodium. This was true for control subjects and those with hyperthyroidism and this would support the view that the intracellular sodium concentration is constant and represents the result of a balance between influx and efflux. In hyperthyroidism this balance persists, but with an abnormally high intracellular sodium concentration. 5. There was a linear relationship between the cell sodium content and the active transport of sodium from the cell in control and hyperthyroid subjects. 6. Triiodothyronine did not produce any change in sodium transport by normal red cells in vitro. 7. It is concluded that there is a depression of the activity of the sodium pump in the red cells of hyperthyroid subjects. This allows the resting intracellular sodium concentration to rise until a new steady state is reached. Evidence is given that these changes are reversed when hyperthyroidism is corrected.

Observations on the Measurement and Regulation of the Sodium Content of Human Erythrocytes

1972 ◽  
Vol 42 (4) ◽  
pp. 447-453 ◽  
Author(s):  
E. K. M. Smith
Keyword(s):  
Linear Correlation ◽  
Sodium Pump ◽  
Potassium Concentration ◽  
Normal Subjects ◽  
Sodium Concentration ◽  
Sodium Content ◽  
Active Sodium ◽  
Sodium Efflux ◽  
Precise Control ◽  
A Cell

1. The sodium concentration within the erythrocytes of 159 subjects was found to be 7·35 ± 1·25 (SD) mmol/litre of cells. 2. In 157 normal subjects, the erythrocyte potassium concentration was 99·08 ± 5·3 (SD) mmol/litre of cells. 3. In the erythrocytes from twenty-seven normal subjects there was a striking linear correlation between the rate constant for active sodium efflux and resting sodium concentration. 4. It is concluded that these studies confirm the assumption that the resting concentration of sodium within a cell is determined by the activity of the sodium pump. What is not known is the mechanism by which this precise control is maintained.

scholarly journals STUDY OF THE EFFECT OF HYPOTHERMIC CONSERVATION ON THE INTRACELLULAR SODIUM CONCENTRATION IN THE ENDOTHELIUM OF CORNEAL TRANSPLANTS

2018 ◽  
Vol 22 (4) ◽  
pp. 433-437
Author(s):  
G. S. Baturina ◽  
I. G. Palchikova ◽  
A. A. Konev ◽  
E. S. Smirnov ◽  
L. E. Katkova ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Plasma Membranes ◽  
Sodium Concentration ◽  
Intracellular Sodium ◽  
Sodium Balance ◽  
Human Cornea ◽  
Sodium Permeability ◽  
Custom Made ◽  
Intracellular Sodium Concentration ◽  
Cornea Endothelium

Endothelial keratoplasty has become the treatment of choice for corneal endothelial dysfunction. Advancements in the surgical treatment of corneal endothelial diseases depend on progress in graft conservation and its related advantages in assessing the suitability of grafts for transplantation. Transport of water and ions by cornea endothelium is important for the optic properties of cornea. In this work, we study the intracellular sodium concentration in cornea endothelial cells in samples of pig cornea that underwent hypothermic conservation for 1 and 10 days and endothelial cells of human cornea grafts after 10-day conservation. The concentration of intracellular sodium in preparations of endothelial cells was assayed using fluorescent dye SodiumGreen. The fluorescent images were analyzed with the custom-made computer program CytoDynamics. An increased level of intracellular sodium was shown in the endothelium after 10-day conservation in comparison with one-day conservation (pig samples). Sodium permeability of pig endothelial cell plasma membranes significantly decreased in these samples. Assessment of intracellular sodium in human cornea endothelium showed a higher level – as was in analogues pig samples of the corneal endothelium. The assay of the intracellular sodium balance concentration established in endothelial cells after hypothermic conservation in mediums L-15 and Optisol-GS showed a significant advantage of specialized me dium Optisol-GS. The balanced intracellular concentration after 10 days of hypothermic conservation was significantly lower in cells incubated at 4 °C in Optisol-GS (L-15, 128 ± 14,  n = 15; Optisol-GS, 108 ± 14, n = 11; mM, p < 0.001). Intracellular sodium concentration could be a useful parameter for assessing cornea endothelium cell viability.

scholarly journals Agonist-dependent Regulation of Renal Na+,K+-ATPase Activity Is Modulated by Intracellular Sodium Concentration

2002 ◽  
Vol 277 (13) ◽  
pp. 11489-11496 ◽  
Author(s):  
Riad Efendiev ◽  
Alejandro M. Bertorello ◽  
Ruben Zandomeni ◽  
Angel R. Cinelli ◽  
Carlos H. Pedemonte
Keyword(s):  
Atpase Activity ◽  
Sodium Concentration ◽  
Intracellular Sodium ◽  
Intracellular Sodium Concentration

Thyroid Nodules in Patients with Acromegaly: Frequency According to the ACR TI-RADS Classification and its Relationship with Disease Activity

2021 ◽  
Author(s):  
Mustafa Can ◽  
Muhammet Kocabaş ◽  
Melia Karakose ◽  
Hatice Caliskan Burgucu ◽  
Zeliha Yarar ◽  
...  
Keyword(s):  
Disease Activity ◽  
Thyroid Nodules ◽  
Thyroid Volume ◽  
Thyroid Stimulating Hormone ◽  
Control Group ◽  
Control Subjects ◽  
Number Of Patients ◽  
Significant Difference ◽  
The Mean ◽  
And Control

Abstract Purpose: In our study, we aimed to determine the frequency of thyroid nodules in patients with acromegaly according to the American College of Radiology (ACR) Thyroid Imaging, Reporting and Data System (TI-RADS) classification and its relationship with acromegaly disease activity. Methods: A total of 56 patients with acromegaly and age, sex, and body mass index matched with 56 healthy control subjects were included in our study. Thyroid-stimulating hormone, free thyroxine, and anti-thyroperoxidase antibody levels of patients and control subjects were measured. In addition, patients and healthy controls were evaluated by ultrasonography to determine thyroid structure, thyroid volume, and thyroid nodules and to make ACR TI-RADS classification. Results: Thyroid nodules were present in 31 (55.4%) of 56 patients in the acromegaly group and 20 (35.7%) of 56 subjects in the control group, and the frequency of thyroid nodules was significantly higher in the acromegaly group (p=0.038). The mean number of nodules in the acromegaly group and control group was 1.27±1.43 and 0.48±0.73, respectively, and the mean number of nodules was significantly higher in the acromegaly group (p=0.003). The number of patients with TI-RADS 1, TI-RADS 2, and TI-RADS 4 nodules in the acromegaly group was higher than the control group (p=0.026, p=0.049, p=0.007, respectively). No difference was found in terms of cytological findings between those who have undergone FNAB in the acromegaly group and control group. Conclusion: In our study, we found that the frequency of thyroid nodules, the number of thyroid nodules, and the number of TI-RADS 1, TI-RADS 2, and TI-RADS 4 nodules increased in patients with acromegaly. There was no significant difference between acromegaly disease activity and thyroid nodule frequency, number of thyroid nodules, and TI-RADS classifications.

scholarly journals Biological strandard for electron microprobe analysis of intracellular sodium concentration.

1976 ◽  
Vol 24 (6) ◽  
pp. 749-751 ◽  
Author(s):  
V S Sottiurai ◽  
R L Malvin ◽  
L F Allard ◽  
W C Bigelow
Keyword(s):  
Electron Microprobe ◽  
Microprobe Analysis ◽  
Electron Microprobe Analysis ◽  
Large Cell ◽  
Sodium Concentration ◽  
Intracellular Sodium ◽  
Cell Populations ◽  
Intracellular Sodium Concentration ◽  
Saline Solutions

A method is described for the determination of the intracellular concentration of sodium in individual cells using the electron microprobe analyzer. This method gives an accuracy equal to that obtained by using flame photometry on tissues with large cell populations. Intracellular sodium was precipitated in the cell by a fixative containing pyroantimonate. Cartilaginous needles from shark fins which were equilibrated in saline solutions of differing concentrations were used as biological standards.

Diphenylhydantoin and intracellular sodium concentration

Neurology ◽  
1969 ◽  
Vol 19 (4) ◽  
pp. 419-419 ◽  
Author(s):  
J. H. Pincus ◽  
M. D. Rawson
Keyword(s):  
Sodium Concentration ◽  
Intracellular Sodium ◽  
Intracellular Sodium Concentration
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