Regulation of cAMP in a lymphocyte preparation isolated from peripheral venous blood in human subjects: the significance of residual thrombocytes, noradrenaline and prostaglandins

1.The aim of the study was to elucidate the mechanism of the previously reported close correlation observed between noradrenaline and cAMP in a lymphocyte preparation (LP) isolated from peripheral venous blood in healthy subjects. A close correlation was also obtained in the present study between lymphocyte noradrenaline and adrenaline and cAMP both in the basal state and after stimulation with isoproterenol (P< 0.05 to 0.007). 2.Although 99% of the thrombocytes were removed from the LP during the washing procedure, LP contained approximately one thrombocyte per lymphocyte. The noradrenaline concentration in LP which could be ascribed to residual thrombocytes, calculated from the average noradrenaline concentration in thrombocytes and the number of thrombocytes in LP, correlated closely to noradrenaline in LP (P< 0.007). 3.To test the hypothesis that noradrenaline in LP was primarily derived from plasma, we studied three patients with phaeochromocytoma, who had high levels of noradrenaline and adrenaline both in plasma and in LP. 4.Further studies showed that the addition of thrombocytes to LP increased cAMP. The response was inhibited by indomethacin, whereas the addition of cimetidine and propranolol had no effect on basal or thrombocyte-stimulated cAMP. 5.We conclude that noradrenaline in LP is a marker of the number of residual thrombocytes. The addition of thrombocytes to LP increased cAMP in lymphocytes. This response was not mediated by catecholamines but possibly by prostaglandins.

Download Full-text

Measurement of 5-Androsten-3β, 17β-diol in Spermatic and Peripheral Venous Blood Samples from the Same Human Subjects by a Radioimmunoassay Method

Hormone Research ◽

10.1159/000178913 ◽

1978 ◽

Vol 9 (4) ◽

pp. 194-200 ◽

Cited By ~ 9

Author(s):

G. Forti ◽

E. Calabresi ◽

P. Giannotti ◽

D. Borrelli ◽

P. Gonnelli ◽

...

Keyword(s):

Human Subjects ◽

Venous Blood ◽

Blood Samples ◽

Peripheral Venous Blood ◽

Radioimmunoassay Method

Download Full-text

PRELIMINARY OBSERVATIONS ON THE RELEASE OF OXYTOCIN AND VASOPRESSIN IN HUMAN SUBJECTS

Journal of Endocrinology ◽

10.1677/joe.0.0210125 ◽

1960 ◽

Vol 21 (1) ◽

pp. 125-128 ◽

Cited By ~ 11

Author(s):

R. R. CHAUDHURY ◽

G. F. JOPLIN

Keyword(s):

Intravenous Injection ◽

Human Subjects ◽

Venous Blood ◽

Blood Levels ◽

Moderate Severity ◽

Peripheral Venous Blood ◽

Vasopressor Activity ◽

Hypothalamic Damage

SUMMARY 1. Oxytocic and vasopressor activity were found in human peripheral venous blood following overnight dehydration and an intravenous injection of nicotine. 2. The quantities are adequate for investigation of hypothalamic damage of moderate severity. 3. The blood levels of oxytocic activity exceed vasopressor activity.

Download Full-text

Regulation of cAMP in a lymphocyte preparation isolated from peripheral venous blood in human subjects: the significance of residual thrombocytes, noradrenaline and prostaglandins

Clinical Science ◽

10.1042/cs19970294 ◽

1998 ◽

Vol 95 (3) ◽

pp. 377 ◽

Cited By ~ 1

Author(s):

Susanne B. SØNDERGAARD ◽

Jens H. KNUDSEN ◽

Niels J. CHRISTENSEN

Keyword(s):

Human Subjects ◽

Venous Blood ◽

Peripheral Venous Blood

Download Full-text

ADRENALINE IN THE HUMAN PANCREAS

Acta Endocrinologica ◽

10.1530/acta.0.0820753 ◽

1976 ◽

Vol 82 (4) ◽

pp. 753-757 ◽

Cited By ~ 1

Author(s):

N. J. Christensen ◽

B. Neubauer

Keyword(s):

Human Subjects ◽

Postmortem Examination ◽

The Body ◽

The Other ◽

Diabetic Patients ◽

Human Pancreas ◽

Noradrenaline Concentration ◽

Tissue Specimens ◽

Noradrenaline And Adrenaline

ABSTRACT The present study describes the results of examinations of the noradrenaline and adrenaline concentrations in the human pancreas as well as in a number of other organs. Tissue specimens were obtained at postmortem examination. Adrenaline was present in small amounts in the cardiovascular system, the liver and the spleen in comparison with the noradrenaline concentration. The pancreas, especially the body of the pancreas, contained, however, considerable amounts of adrenaline. The average adrenaline concentration was approximately 20 times higher in the pancreas than in the other organs examined. The greatest concentration of adrenaline was found in the posterior and superior parts of the body of the pancreas. There was no relationship between the cause of death in the human subjects and the adrenaline concentration in the pancreas and large amounts of adrenaline were also found in tissue specimens of pancreas obtained from long-term diabetic patients. Adrenaline was present in the pancreas of the rat, dog and rabbit but in small amounts in comparison with the noradrenaline concentration.

Download Full-text

Platelet Hyperaggregability in Ischemic Cerebrovascular Disease and Effects of Aspirin

Thrombosis and Haemostasis ◽

10.1055/s-0038-1657237 ◽

1982 ◽

Vol 48 (02) ◽

pp. 117-119 ◽

Cited By ~ 6

Author(s):

M Kusunoki ◽

K Kimura ◽

K Nagatsuka ◽

Y Isaka ◽

O Uyama ◽

...

Keyword(s):

Cerebrovascular Disease ◽

Venous Blood ◽

Second Phase ◽

Cerebral Angiogram ◽

Peripheral Venous Blood ◽

Chronic Stage ◽

Ischemic Cerebrovascular Disease ◽

Carotid Arterial ◽

Platelet Hyperaggregability ◽

The Brain

SummaryPlatelet aggregation was studied in 24 patients in the chronic stage of ischemic cerebrovascular disease (CVD), with cerebral affluent and effluent blood, i.e., carotid arterial and internal jugular venous blood, and also with peripheral venous blood. Aggregation tests were performed at various final concentrations of sodium arachidonate (A.A.) and ADP. In 17 patients, not taking aspirin, platelet aggregability in jugular venous blood was significantly accentuated compared with that in arterial and peripheral venous blood. This tendency was more marked in the patients with cerebral artery stenosis and/or occlusion than in those with normal cerebral angiogram. In 7 patients taking 500 mg or more oral aspirin, aggregation differences across the brain were not observed and A.A. aggregation and the second phase of ADP aggregation were completely suppressed. These results suggest that a prophylactic administration of aspirin may be beneficial for patients in chronic stage of CVD.

Download Full-text