scholarly journals Cell membrane changes of structure and function in protein kinase inhibitor‐induced polyploid cells

2000 ◽  
Vol 33 (1) ◽  
pp. 29-38 ◽  
Author(s):  
Z. P. Zong ◽  
K. Fujikawa‐Yamamoto ◽  
A. L. Li ◽  
N. Yamaguchi ◽  
Y. G. Chang ◽  
...  
Keyword(s):  
Protein Kinase ◽  
Cell Membrane ◽  
Kinase Inhibitor ◽  
Structure And Function ◽  
Protein Kinase Inhibitor ◽  
Polyploid Cells ◽  
Membrane Changes ◽  
And Function

scholarly journals Reproductive Function in Protein Kinase Inhibitor-Deficient Mice

2001 ◽  
Vol 21 (12) ◽  
pp. 3959-3963 ◽  
Author(s):  
Mouna Belyamani ◽  
Esha A. Gangolli ◽  
Rejean L. Idzerda
Keyword(s):  
Protein Kinase ◽  
Knockout Mice ◽  
Nuclear Export ◽  
Kinase Inhibitor ◽  
Physiological Role ◽  
Reproductive Function ◽  
Protein Kinase Inhibitor ◽  
Double Knockout ◽  
Genes Encoding ◽  
And Function

ABSTRACT The protein kinase inhibitor (PKI) family includes three genes encoding small, heat-stable inhibitors of the cyclic AMP-dependent kinase PKA. Each PKI isoform contains a PKA inhibitory domain and a nuclear export domain, enabling PKI to both inhibit PKA and remove it from the nucleus. The PKIβ isoform, also known as testis PKI, is highly expressed in germ cells of the testis and is found at more modest levels in other tissues. In order to investigate its physiological role, we have generated PKIβ knockout mice by gene targeting. These mice exhibit a partial loss of PKI activity in testis but remain fertile with normal testis development and function. PKIβ knockout females also reproduce normally. The PKIβ mutants were crossed with our previously derived PKIα mutants to obtain double-knockout mice. Remarkably, these mice are also viable and fertile with no obvious physiological defects in either males or females.

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