The effect of immunotherapy on the serum levels of eosinophil cationic protein in seasonal allergic rhinitis

Abstract Background The aim was to determine the level of inflammatory cytokines, eosinophil cationic protein and IgE in allergic rhinitis (AR) patients. Subjects and methods Blood samples were taken from 88 AR patients and 88 healthy controls (HC). Each sample was analysed for eosinophil counts by flow cytometry, IgE by ECLIA, ECP, IL-17, and IL-33 by using ELISA test. Results There was no significant difference between AR patients and the control group in age and gender. Levels of eosinophils, IgE, ECP, IL-17, IL-33 and the total symptom scores were significantly higher in AR patients than the HC (P = 0.0001). Serum ECP correlated with IL-17 (P = 0.041, r = 0.42), IL-33 (P = 0.0001, r = 080), and IgE levels (P = 0.017, r = 0.45) in the R patients. There was no correlation between IL-17 and IL-33. There was a correlation between symptom scores and eosinophils (P = 0.026, r = 0.52), and IgE (P = 0.001, r = 0.60) in the patients. No correlation was observed between symptom scores and ECP, IL-17, and IL-33 in the AR patient. Conclusions Patients with AR have significant higher serum levels of ECP, IL-17, and IL-33 than healthy controls. This indicates that these markers could be used to in order to diagnose AR and to monitor disease. Inhibitory molecules to IL-17 and IL-33 may be considered as novel treatment strategies.

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Effect of Immunotherapy on Serum Levels of Eosinophil Cationic Protein in Perennial Allergic Rhinitis

Annals of Otology Rhinology & Laryngology ◽

10.1177/000348949710601007 ◽

1997 ◽

Vol 106 (10) ◽

pp. 848-853 ◽

Cited By ~ 4

Author(s):

Yoshiaki Nakai ◽

Hirokazu Sakamoto ◽

Yoshihiro Ohashi ◽

Yasushi Kakinoki ◽

Akifumi Kato ◽

...

Keyword(s):

Allergic Rhinitis ◽

Clinical Effect ◽

Eosinophil Cationic Protein ◽

Serum Levels ◽

Untreated Group ◽

Perennial Allergic Rhinitis ◽

Cationic Protein ◽

The Mean ◽

Eosinophil Activation ◽

Rate Of Activation

Eosinophil cationic protein (ECP) levels in the serum of clotted blood could reflect the rate of activation of circulating eosinophils. We investigated the serum ECP levels in patients with perennial allergic rhinitis, with special reference to the effect of immunotherapy on the serum ECP levels. Serum ECP levels in untreated patients with perennial allergic rhinitis are significantly higher than those of nonatopic volunteers. Therefore, this elevation in the untreated patients represents an ongoing inflammation occurring in allergic rhinitis. The mean serum ECP level of a 1-year immunotherapy group was significantly higher than that of the nonatopic group, and was not different from that of the untreated group. In contrast, the mean serum ECP level in patients who had more than 2 years of immunotherapy was significantly lower than that of the untreated group, and was not different from that of the nonatopic group. Additionally, serum ECP levels were significantly correlated with the duration of immunotherapy. These findings suggest that activation of circulating eosinophils decreases gradually during immunotherapy, but this inhibition becomes apparent only after 2 years of immunotherapy. The control of circulating eosinophil activation might be one of the important working mechanisms behind the clinical effect of immunotherapy.

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Course of eosinophil cationic protein in the nasal secretions of patients with seasonal allergic rhinitis

Clinical Otolaryngology ◽

10.1046/j.1365-2273.2000.00358-32.x ◽

2000 ◽

Vol 25 (4) ◽

pp. 331-331

Author(s):

B. Kremer ◽

L. Klimek ◽

R. Mosges

Keyword(s):

Allergic Rhinitis ◽

Seasonal Allergic Rhinitis ◽

Eosinophil Cationic Protein ◽

Cationic Protein ◽

Nasal Secretions

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Some features of hematological parameters and production of cytokines regulating inflammation in allergic rhinitis

Rossiiskii Allergologicheskii ZHurnal ◽

10.36691/rja1444 ◽

2021 ◽

Author(s):

Natalia S. Iraklionova ◽

Eleonora B. Belan

Keyword(s):

Allergic Rhinitis ◽

Hematological Parameters ◽

Eosinophil Cationic Protein ◽

Serum Levels ◽

Normal Serum ◽

Immune Reactivity ◽

Cationic Protein ◽

Total Ige ◽

Protein Levels ◽

Serum Total Ige

Backgraund: Allergic rhinitis (AR) is a common IgE-mediated disease. Multiple mechanisms are involved in the regulation of IgE synthesis, and cytokines produced by immune cells play an important role in this process. In addition, the study of the features of immunological reactivity in seasonal AR (SAR) is of interest both getting of new data about pathogenesis of the disease and optimization of the treatment. Aims: To study the features of the cytokine status and hematological parameters in patients with SAR outside the period of exacerbation. Materials and methods: 43 adult patients with SAR (stage of remission) have been included in the study, and 47 conditionally healthy patients have formed the comparison group; perennial symptoms and/or sensitization to perennial allergens were considered as exclusion criteria. Complete blood cell count and serum IL-4, IL-5, IL-6, IL-8, IL-10, IL-18, MCP-1, total IgE, eosinophil cationic protein levels were measured. Results: The remission stage in patients with SAR is characterized by higher serum levels of total IgE and IL-8 compared with the group of healthy patients. Normal serum total IgE level in patients with SAR in remission is associated with activation of the monocyte-macrophage link (increased serum levels of MCP-1, IL-6, IL-8, absolute and relative numbers of monocytes). Increased serum total IgE level is associated with the predominant signs of the Th2-phenotype of the immune response (increased in the serum levels of IL-4, IL-5). Conclusions: Immune reactivity of patients with natural remission of SAR is characterized by Th1-phenotype features if serum level of IgE is normal and Th2-ones if IgE is increased.

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Tryptase, eosinophil cationic protein (ECP) and eosinophil counts in seasonal allergic rhinitis

Journal of Allergy and Clinical Immunology ◽

10.1016/s0091-6749(02)81390-2 ◽

2002 ◽

Vol 109 (1) ◽

pp. S96-S96

Author(s):

Asli Gelincik ◽

Suna Büyüköztürk ◽

Bahauddin Çolakoϖlu ◽

Murat Dal ◽

Aytug Akkor

Keyword(s):

Allergic Rhinitis ◽

Seasonal Allergic Rhinitis ◽

Eosinophil Cationic Protein ◽

Cationic Protein ◽

Eosinophil Counts

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Eosinophil Cationic Protein in Nasal Secretions and Blood Serum in Grass-Pollen Allergic Rhinitis

American Journal of Rhinology ◽

10.2500/105065896782159738 ◽

1996 ◽

Vol 10 (5) ◽

pp. 319-322 ◽

Cited By ~ 7

Author(s):

L. Klimek ◽

H. Riechelmann ◽

R. Amedee

Keyword(s):

Allergic Rhinitis ◽

Blood Serum ◽

Healthy Volunteers ◽

Grass Pollen ◽

Eosinophil Cationic Protein ◽

Serum Levels ◽

Eosinophilic Inflammation ◽

Cationic Protein ◽

Nasal Secretions ◽

Pollen Exposure

Concentration of the Eosinophil Cationic Protein (ECP) measured in different body fluids has been demonstrated to be a good marker of eosinophilic inflammation. In allergic asthma, ECP levels in both broncho-alveolar-lavage (BAL) fluid and serum can be used to monitor disease activity. In allergic rhinitis, the value of ECP determinations in serum and nasal secretions was not directly compared, so far. In the present study, ECP levels in blood serum (Se) and nasal secretions (NS) of grass pollen allergic and healthy individuals under pollen exposure were analyzed. Forty-three grass-pollen allergic subjects and 19 healthy volunteers were included. Grass-pollen counts were measured using Burkhard traps and a symptom scoring performed. In the allergic subjects, the mean ECP concentration in nasal secretions was 789 ± 515 ng/mL compared with 12.1 ± 7.4 ng/mL in serum; in the healthy volunteers it was 29.6 ± 15.4 ng/mL in NS compared to 9.4 ± 6.9 ng/mL in Se. ECP concentration in NS was significantly higher in allergic than in healthy subjects (P < 0.001) and in both significantly higher than in Se (P < 0.01). ECP concentration in Se did not differ significantly in patients and healthy volunteers (P > 0.05). In the allergic subjects, ECP concentration in NS but not in Se was closely correlated to symptom score and pollen exposure. We conclude that ECP levels in NS are a better marker of eosinophilic inflammation in allergic rhinitis than ECP serum levels.

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