Some features of hematological parameters and production of cytokines regulating inflammation in allergic rhinitis

2021 ◽  
Author(s):  
Natalia S. Iraklionova ◽  
Eleonora B. Belan
Keyword(s):  
Allergic Rhinitis ◽  
Hematological Parameters ◽  
Eosinophil Cationic Protein ◽  
Serum Levels ◽  
Normal Serum ◽  
Immune Reactivity ◽  
Cationic Protein ◽  
Total Ige ◽  
Protein Levels ◽  
Serum Total Ige

Backgraund: Allergic rhinitis (AR) is a common IgE-mediated disease. Multiple mechanisms are involved in the regulation of IgE synthesis, and cytokines produced by immune cells play an important role in this process. In addition, the study of the features of immunological reactivity in seasonal AR (SAR) is of interest both getting of new data about pathogenesis of the disease and optimization of the treatment. Aims: To study the features of the cytokine status and hematological parameters in patients with SAR outside the period of exacerbation. Materials and methods: 43 adult patients with SAR (stage of remission) have been included in the study, and 47 conditionally healthy patients have formed the comparison group; perennial symptoms and/or sensitization to perennial allergens were considered as exclusion criteria. Complete blood cell count and serum IL-4, IL-5, IL-6, IL-8, IL-10, IL-18, MCP-1, total IgE, eosinophil cationic protein levels were measured. Results: The remission stage in patients with SAR is characterized by higher serum levels of total IgE and IL-8 compared with the group of healthy patients. Normal serum total IgE level in patients with SAR in remission is associated with activation of the monocyte-macrophage link (increased serum levels of MCP-1, IL-6, IL-8, absolute and relative numbers of monocytes). Increased serum total IgE level is associated with the predominant signs of the Th2-phenotype of the immune response (increased in the serum levels of IL-4, IL-5). Conclusions: Immune reactivity of patients with natural remission of SAR is characterized by Th1-phenotype features if serum level of IgE is normal and Th2-ones if IgE is increased.

The effect of immunotherapy on the serum levels of eosinophil cationic protein in seasonal allergic rhinitis

1997 ◽  
Vol 22 (2) ◽  
pp. 100-105 ◽  
Author(s):  
Y. OHASHI ◽  
Y. NAKAI ◽  
Y. KAKINOKI ◽  
Y. OHNO ◽  
H. OKAMOTO ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Seasonal Allergic Rhinitis ◽  
Eosinophil Cationic Protein ◽  
Serum Levels ◽  
Cationic Protein

scholarly journals Differentiating the endotypes in allergic rhinitis

2021 ◽  
Vol 36 (2) ◽  
pp. 92-97
Author(s):  
A. V. Klimov ◽  
Z. V. Salahutdinova ◽  
N. A. Pronina ◽  
G. A. Kuznetsov
Keyword(s):  
Allergic Rhinitis ◽  
Eosinophil Cationic Protein ◽  
Skin Tests ◽  
Research Trend ◽  
General Group ◽  
Cationic Protein ◽  
Total Ige ◽  
Interleukin 5 ◽  
Local Allergic Rhinitis ◽  
History Of

Aim. The aim of the study was to differentiate the endotypes in allergic rhinitis by key allergy markers in a mixed group of patients.Material and Methods. The study comprised a total of 48 patients, men and women, aged 18-60 years suffering from three endotypes of allergic rhinitis including the classic, local, and dual allergic rhinitis. The standard diagnostics of allergic rhinitis included taking a history of allergies, family history of allergic disease, video rhinoscopy, serum total IgE level assessment, allergy skin tests to house dust mite and pollen allergens, and study of eosinophilic inflammation parameters (eosinophil cationic protein, interleukin-5 (IL5), and eosinophil counts in blood and nasal secretion).Results. Based on total IgE level, the general group of patients was divided to two subgroups: subgroup 1 comprised patients with high IgE level (n = 22); subgroup 2 comprised patients with low IgE level (n = 26). Most of patients in these groups had contradictory results of allergy skin tests i.e. positive allergy skin test results in case of high IgE level (group 1) and vice versa. Cluster analysis-based exminations of general group allowed to categorize three subgroups of patients: patients with classic allergic rhinitis (n = 22), local allergic rhinitis (n = 22), and dual allergic rhinitis (n = 4). Besides, an increased rate of anxiety disorder was found in patients with local allergic rhinitis (p < 0.001).Conclusion. The obtained data showed promise for a new research trend in studying allergic rhinitis endotypes, namely: investigation of neuroimmune relationships in allergic tolerance disruption in the presence of this pathology.

scholarly journals Evidence for eosinophil and IL-17 mediated inflammation in allergic rhinitis

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
Kawa Amin ◽  
Sulaf Mosa Issa ◽  
Kosar Mohammad Ali ◽  
Muaid Ismiel Aziz ◽  
Huner Mohamed Hama Amieen ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Eosinophil Cationic Protein ◽  
Treatment Strategies ◽  
Serum Levels ◽  
Elisa Test ◽  
Control Group ◽  
Healthy Controls ◽  
Cationic Protein ◽  
Significant Difference ◽  
Symptom Scores

Abstract Background The aim was to determine the level of inflammatory cytokines, eosinophil cationic protein and IgE in allergic rhinitis (AR) patients. Subjects and methods Blood samples were taken from 88 AR patients and 88 healthy controls (HC). Each sample was analysed for eosinophil counts by flow cytometry, IgE by ECLIA, ECP, IL-17, and IL-33 by using ELISA test. Results There was no significant difference between AR patients and the control group in age and gender. Levels of eosinophils, IgE, ECP, IL-17, IL-33 and the total symptom scores were significantly higher in AR patients than the HC (P = 0.0001). Serum ECP correlated with IL-17 (P = 0.041, r = 0.42), IL-33 (P = 0.0001, r = 080), and IgE levels (P = 0.017, r = 0.45) in the R patients. There was no correlation between IL-17 and IL-33. There was a correlation between symptom scores and eosinophils (P = 0.026, r = 0.52), and IgE (P = 0.001, r = 0.60) in the patients. No correlation was observed between symptom scores and ECP, IL-17, and IL-33 in the AR patient. Conclusions Patients with AR have significant higher serum levels of ECP, IL-17, and IL-33 than healthy controls. This indicates that these markers could be used to in order to diagnose AR and to monitor disease. Inhibitory molecules to IL-17 and IL-33 may be considered as novel treatment strategies.

Effect of Immunotherapy on Serum Levels of Eosinophil Cationic Protein in Perennial Allergic Rhinitis

1997 ◽  
Vol 106 (10) ◽  
pp. 848-853 ◽  
Author(s):  
Yoshiaki Nakai ◽  
Hirokazu Sakamoto ◽  
Yoshihiro Ohashi ◽  
Yasushi Kakinoki ◽  
Akifumi Kato ◽  
...  
Keyword(s):  
Allergic Rhinitis ◽  
Clinical Effect ◽  
Eosinophil Cationic Protein ◽  
Serum Levels ◽  
Untreated Group ◽  
Perennial Allergic Rhinitis ◽  
Cationic Protein ◽  
The Mean ◽  
Eosinophil Activation ◽  
Rate Of Activation

Eosinophil cationic protein (ECP) levels in the serum of clotted blood could reflect the rate of activation of circulating eosinophils. We investigated the serum ECP levels in patients with perennial allergic rhinitis, with special reference to the effect of immunotherapy on the serum ECP levels. Serum ECP levels in untreated patients with perennial allergic rhinitis are significantly higher than those of nonatopic volunteers. Therefore, this elevation in the untreated patients represents an ongoing inflammation occurring in allergic rhinitis. The mean serum ECP level of a 1-year immunotherapy group was significantly higher than that of the nonatopic group, and was not different from that of the untreated group. In contrast, the mean serum ECP level in patients who had more than 2 years of immunotherapy was significantly lower than that of the untreated group, and was not different from that of the nonatopic group. Additionally, serum ECP levels were significantly correlated with the duration of immunotherapy. These findings suggest that activation of circulating eosinophils decreases gradually during immunotherapy, but this inhibition becomes apparent only after 2 years of immunotherapy. The control of circulating eosinophil activation might be one of the important working mechanisms behind the clinical effect of immunotherapy.

Eosinophil Cationic Protein in Nasal Secretions and Blood Serum in Grass-Pollen Allergic Rhinitis

1996 ◽  
Vol 10 (5) ◽  
pp. 319-322 ◽  
Author(s):  
L. Klimek ◽  
H. Riechelmann ◽  
R. Amedee
Keyword(s):  
Allergic Rhinitis ◽  
Blood Serum ◽  
Healthy Volunteers ◽  
Grass Pollen ◽  
Eosinophil Cationic Protein ◽  
Serum Levels ◽  
Eosinophilic Inflammation ◽  
Cationic Protein ◽  
Nasal Secretions ◽  
Pollen Exposure

Concentration of the Eosinophil Cationic Protein (ECP) measured in different body fluids has been demonstrated to be a good marker of eosinophilic inflammation. In allergic asthma, ECP levels in both broncho-alveolar-lavage (BAL) fluid and serum can be used to monitor disease activity. In allergic rhinitis, the value of ECP determinations in serum and nasal secretions was not directly compared, so far. In the present study, ECP levels in blood serum (Se) and nasal secretions (NS) of grass pollen allergic and healthy individuals under pollen exposure were analyzed. Forty-three grass-pollen allergic subjects and 19 healthy volunteers were included. Grass-pollen counts were measured using Burkhard traps and a symptom scoring performed. In the allergic subjects, the mean ECP concentration in nasal secretions was 789 ± 515 ng/mL compared with 12.1 ± 7.4 ng/mL in serum; in the healthy volunteers it was 29.6 ± 15.4 ng/mL in NS compared to 9.4 ± 6.9 ng/mL in Se. ECP concentration in NS was significantly higher in allergic than in healthy subjects (P < 0.001) and in both significantly higher than in Se (P < 0.01). ECP concentration in Se did not differ significantly in patients and healthy volunteers (P > 0.05). In the allergic subjects, ECP concentration in NS but not in Se was closely correlated to symptom score and pollen exposure. We conclude that ECP levels in NS are a better marker of eosinophilic inflammation in allergic rhinitis than ECP serum levels.

scholarly journals Role of Leptin/Osteopontin Axis in the Function of Eosinophils in Allergic Rhinitis with Obesity

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Wenlong Liu ◽  
Qingxiang Zeng ◽  
Yanqiu Chen ◽  
Ren Zhong Luo
Keyword(s):  
Allergic Rhinitis ◽  
Clinical Symptoms ◽  
Eosinophil Cationic Protein ◽  
Pi3k Pathway ◽  
Intercellular Adhesion Molecule ◽  
Intercellular Adhesion Molecule 1 ◽  
Cationic Protein ◽  
Protein Levels ◽  
Eosinophil Apoptosis

Background. Allergic rhinitis (AR) is characterized by tissue and blood eosinophilia. Previous studies showed enhanced eosinophilia in allergic rhinitis patients with obesity, suggesting an association between obesity and eosinophilia. However, the interaction and mechanism between obesity and eosinophilia is still unclear. Methods. We recruited thirty AR children and 30 controls in this study. Expression of leptin and osteopontin (OPN) proteins in serum was detected, and correlation analysis with eosinophilia was performed. The effect of leptin or OPN on eosinophil apoptosis, adhesion, migration, and activation of eosinophil was examined. Ovalbumin-sensitized mice were established to prove the role of obesity on eosinophil regulation by leptin and OPN. Results. We found that upregulated serum and nasal leptin and OPN expression in AR were positively correlated with eosinophilia and eosinophil cationic protein levels. Leptin or OPN inhibited eosinophil apoptosis, demonstrated as inhibited DNA fragmentation and phosphatidylserine (PS) redistribution (P<0.05). Leptin and OPN promote expression of cluster of differentiation 18 (CD-18) and intercellular adhesion molecule 1 (ICAM-1) and inhibit expression of ICAM-1 and L-selectin by eosinophils, which contribute to the adhesion of eosinophils. Leptin and OPN mediated migration and activation of eosinophil through phosphatidylinositol-3-OH kinase (PI3K) pathway. Obese AR mice presented with more severe eosinophilia and symptoms compared with nonobese AR mice or control mice. Immunochemistry staining of leptin and OPN of nasal turbinate in obese AR mice was also stronger than those in nonobese AR mice or control mice. Anti-OPN, anti-leptin, and anti-α4 treatments reduce nasal eosinophilia inflammation and clinical symptoms in model mice. Conclusion. Our results suggested that in an obese state, upregulation of leptin and OPN regulates apoptosis, adhesion, migration, and activation of eosinophils, and this process may be mediated by the PI3K and anti-α4 pathways.

Eosinophil cationic protein in atopic dermatitis: changes of serum levels by the anti-allergic agent ketotifen *

1994 ◽  
Vol 3 (1) ◽  
pp. 59-62 ◽  
Author(s):  
Kouichi Ikai ◽  
Atsuhiko Ogino ◽  
Ikuko Furukawa ◽  
Motoaki Ozaki ◽  
Mayumi Fujita ◽  
...  
Keyword(s):  
Atopic Dermatitis ◽  
Eosinophil Cationic Protein ◽  
Serum Levels ◽  
Cationic Protein
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