Residual transfusion risk for hepatitis B and C in southern Brazil, 1991-99

2001 ◽  
Vol 8 (1) ◽  
pp. 78-82 ◽  
Author(s):  
E. J. Kupek
Keyword(s):  
2014 ◽  
Vol 47 (5) ◽  
pp. 552-558 ◽  
Author(s):  
Saulo Martins ◽  
Andréa do Livramento ◽  
Michelle Andrigueti ◽  
Iara Fabricia Kretzer ◽  
Marcos José Machado ◽  
...  

2011 ◽  
Vol 15 (2) ◽  
pp. 159-162 ◽  
Author(s):  
Fernando Herz Wolff ◽  
Sandra Costa Fuchs ◽  
Ajacio B.M. Brandão

2014 ◽  
Vol 18 (1) ◽  
pp. 8-12 ◽  
Author(s):  
Cleverson Leitão ◽  
Denis Ueda ◽  
Anna Carolina de Moraes Braga ◽  
Angelica B.W. Boldt ◽  
Iara J.T. Messias-Reason
Keyword(s):  

2020 ◽  
Vol 81 (2-3) ◽  
pp. 79-84
Author(s):  
Jonas Michel Wolf ◽  
Vagner Reinaldo Zingalli Bueno Pereira ◽  
Patrícia Andréia Zanetti Ballardin Roncato ◽  
André Castagna Wortmann ◽  
Glaucia Zuleide Stumm ◽  
...  

2013 ◽  
Vol 46 (4) ◽  
pp. 493-497 ◽  
Author(s):  
Fabiana Schuelter-Trevisol ◽  
Geisiane Custodio ◽  
Ana Carolina Barreto da Silva ◽  
Mikely Byala de Oliveira ◽  
Audrei Wolfart ◽  
...  

2013 ◽  
Vol 46 (4) ◽  
pp. 397-402 ◽  
Author(s):  
Angelica Luciana Nau ◽  
Leonardo Fayad ◽  
Cesar Lazzarotto ◽  
Maria Beatriz Cacese Shiozawa ◽  
Esther Buzaglo Dantas-Correa ◽  
...  

2013 ◽  
Vol 50 (3) ◽  
pp. 219-225 ◽  
Author(s):  
Carlos Eduardo BECKER ◽  
Nelson Alexandre KRETZMANN ◽  
Angelo Alves de MATTOS ◽  
Ana Beatriz Gorini da VEIGA

Context Hepatitis B virus (HBV) can cause fulminant hepatitis, cirrhosis and hepatocellular carcinoma, and is one of the most common causes of acute and chronic liver failure. The genetic variants of HBV can be decisive for the evolution of these diseases as well as for the election of therapy. Objectives The aim of this study was to evaluate and standardize an in house methodology based on the analysis of the melting curve polymerase chain reaction (PCR) of real-time (qPCR) to screen for genotypes A, D and F of HBV in patients from a hospital in Rio Grande do Sul, Brazil. Methods We evaluated 104 patients presumably with HBV chronic infection. Viral DNA was extracted from plasma and viral genotypes and different mutations were determined using PCR-based protocols. Results A PCR-based methodology was standardized for the analysis of genotypes A, D and F of HBV. The technique was based in a nested PCR with the final step consisting of a multiplex real-time PCR, using the melting curve as a tool for the differentiation of fragments. A higher frequency of genotype D (44.4%), followed by genotype A (22.2%) and genotype F (3.7%) was observed. Conclusion The standardized assay, a nested PCR-multiplex qPCR using specific primers, provides a rapid and accurate method for the differentiation of HBV genotypes that are more frequent in Southern Brazil – A, D and F. This method can be applied in the clinical practice.


2016 ◽  
Vol 111 (4) ◽  
pp. 252-257 ◽  
Author(s):  
Camila V Pereira ◽  
Cristiane Valle Tovo ◽  
Thiago K Grossmann ◽  
Henrique Mirenda ◽  
Bruna B Dal-Pupo ◽  
...  

PLoS ONE ◽  
2015 ◽  
Vol 10 (8) ◽  
pp. e0135816 ◽  
Author(s):  
Carolina Souza Gusatti ◽  
Cintia Costi ◽  
Maria Laura Halon ◽  
Tarciana Grandi ◽  
Arlete Ferrari Rech Medeiros ◽  
...  

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