Comparison of homology and linkage of random amplified polymorphic DNA (RAPD) markers between individual trees of Scots pine ( Pinus sylvestris L.)

1999 ◽  
Vol 8 (1) ◽  
pp. 15-22 ◽  
Author(s):  
P. Hurme ◽  
O. Savolainen
Keyword(s):  
Pinus Sylvestris ◽  
Scots Pine ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Individual Trees

scholarly journals Genetic divergence of Scots pine (Pinus sylvestris L.) populations in Serbia revealed by RAPD

2011 ◽  
Vol 63 (2) ◽  
pp. 371-380 ◽  
Author(s):  
A. Lucic ◽  
V. Isajev ◽  
L. Rakonjac ◽  
Danijela Ristic ◽  
Marija Kostadinovic ◽  
...  
Keyword(s):  
Pinus Sylvestris ◽  
Scots Pine ◽  
Genetic Divergence ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna

The ability of random amplified polymorphic DNA (RAPD) to distinguish among Scots pine populations from Serbia was evaluated. Sixteen arbitrary 10-mer primers employed in the analysis produced 54 fragments of which 21 were polymorphic (38.89%). Certain rare and genotype-specific bands were identified which could be effectively used to distinguish between the populations. Polymorphism in RAPD markers among P. sylvestris populations was high and sufficient to distinguish each of the populations. The results obtained suggest that RAPD markers are valuable for the genetic divergence estimation in Pinus sylvestris and for the study of divergence among populations.

scholarly journals Assessment of genetic diversity among surian Toona sinensis Roem in progenies test using random amplified polymorphic DNA markers

2018 ◽  
Vol 22 (1) ◽  
pp. 22
Author(s):  
Jayusman Jayusman ◽  
Muhammad Na’iem ◽  
Sapto Indrioko ◽  
Eko Bhakti Hardiyanto ◽  
ILG Nurcahyaningsih
Keyword(s):  
Genetic Diversity ◽  
Dna Markers ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Management Strategies ◽  
Genetic Distances ◽  
Sampled Data ◽  
Toona Sinensis ◽  
The Mean ◽  
Individual Trees

Surian Toona sinensis Roem is one of the most widely planted species in Indonesia. This study aimed to estimate the genetic diversity between a number of surian populations in a progeny test using RAPD markers, with the goal of proposing management strategies for a surian breeding program. Ninety-six individual trees from 8 populations of surian were chosen as samples for analysis. Eleven polymorphic primers (OP-B3, OP-B4, OP-B10, OP-H3, OP-Y6, OP-Y7, OP-Y8, OP-Y10, OP-Y11, OP-Y14, and OP-06) producing reproducible bands were analyzed for the 96 trees, with six trees per family sampled. Data were analyzed using GenAlEx 6.3, NTSYS 2.02. The observed percentage of polymorphic loci ranged from 18.2% to 50%. The mean level of genetic diversity among the surian populations was considered to be moderate (He 0.304). Cluster analysis grouped the genotypes into two main clusters, at similarity levels of 0.68 and 0.46. The first two axes of the PCoA explained 46.16% and 25.54% of the total variation, respectively. The grouping of samples into clusters and subclusters did not correspond with family and their distances, but the grouping was in line with the genetic distances of the samples.

Random amplified polymorphic DNA markers linked to quantitaive traits loci in Scots pine (Pinus sylvestris L.)

2011 ◽  
Vol 72 (3) ◽  
pp. 205-211
Author(s):  
Iwona Szyp-Borowska ◽  
Joanna Ukalska ◽  
Joanna Simińska
Keyword(s):  
Pinus Sylvestris ◽  
Scots Pine ◽  
Dna Markers ◽  
Random Amplified Polymorphic Dna

Markery RAPD sprzężone z genami cech ilościowych sosny zwyczajnej (Pinus sylvestrisL.)

EVALUATION OF PTYCHOBOTHRIDEAN CESTODES USING RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD) MARKERS FROM FRESH WATER FISHES IN GODAVARI BASIN (M.S.) INDIA

2017 ◽  
Vol 23 (2) ◽  
Author(s):  
SUNITA BORDE ◽  
ASAWARI FARTADE ◽  
AMOL THOSAR ◽  
RAHUL KHAWAL
Keyword(s):  
Fresh Water ◽  
Rapd Markers ◽  
Random Amplified Polymorphic Dna ◽  
Band Pattern ◽  
Genomic Variation ◽  
Polymorphic Band ◽  
Rapd Profile ◽  
Band Size ◽  
Pcr Product ◽  
The Common

Ptychobothridean genera like Senga and Circumoncobothrium are the common parasites of fresh water fishes. The genotypic study of these parasites was taken by RAPD. The RAPD profile of these two parasites were not similar to each other as depicted by the band pattern in picture. These results suggest the presence of inter-specific polymorphism among cestode parasites of two different genera for RAPD analysis. The present study demonstrated that genetic differentiation of cestode parasites could be accomplished on the basis of genomic variation with polymorphic band pattern using RAPD. All the detected bands (PCR product) were polymorphic and band size ranged from 500-5000 bp in length. The RAPD of profiles using GBO-31, GBO-32, GBO-33, GBO-34, GBO-35 and GBO-36. Primers were able to characterize inter-specific polymorphism among the two genus ( Senga and Circumoncobothrium ). Genetic analysis suggests that Senga and Circumoncobothrium show genetic diversity with respect to RAPD patterns using all the six primers used for the present study. The genetic distance between the analyzed genuses ranged from 0.14 to 0.80. The differentiation of the two parasites on the basis of genetic markers could greatly facilitate study on the biology of these parasites.

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