ABSTRACT
Six mutants (B1 to B6) that grew poorly in air on BG11 agar plates buffered at pH 8.0 were rescued after mutations were introduced intondhB of wild-type (WT) Synechocystis sp. strain PCC 6803. In these mutants and a mutant (M55) lacking ndhB, CO2 uptake was much more strongly inhibited than HCO3
− uptake, i.e., the activities of CO2 and HCO3
− uptake in B1 were 9 and 85% of those in the WT, respectively. Most of the mutants grew very slowly or did not grow at all at pH 6.5 or 7.0 in air, and their ability to grow under these conditions was correlated with CO2 uptake capacity. Detailed studies of B1 and M55 indicated that the mutants grew as fast as the WT in liquid at pH 8.0 under air, although they grew poorly on agar plates. The contribution of CO2 uptake appears to be larger on solid medium. Five mutants were constructed by inactivating each of the fivendhD genes in Synechocystis sp. strain PCC 6803. The mutant lacking ndhD3 grew much more slowly than the WT at pH 6.5 under 50 ppm CO2, although otherndhD mutants grew like the WT under these conditions and showed low affinity for CO2 uptake. These results indicated the presence of multiple NAD(P)H dehydrogenase type I complexes with specific roles.
A new type of glutamine synthetase in cyanobacteria: the protein encoded by the glnN gene supports nitrogen assimilation in Synechocystis sp. strain PCC 6803.