OsPNH1regulates leaf development and maintenance of the shoot apical meristem in rice

In this paper we describe the expression patterns of a family of homeobox genes in maize and their relationship to organogenic domains in the vegetative shoot apical meristem. These genes are related by sequence to KNOTTED1, a gene characterized by dominant neomorphic mutations which perturb specific aspects of maize leaf development. Four members of this gene family are expressed in shoot meristems and the developing stem, but not in determinate lateral organs such as leaves or floral organs. The genes show distinct expression patterns in the vegetative shoot apical meristem that together predict the site of leaf initiation and the basal limit of the vegetative ‘phytomer’ or segmentation unit of the shoot. These genes are also expressed in the inflorescence and floral meristems, where their patterns of expression are more similar, and they are not expressed in root apical meristems. These findings are discussed in relation to other studies of shoot apical meristem organization as well as possible commonality of homeobox gene function in the animal and plant kingdoms.

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Behavior of organelles and their nucleoids in the shoot apical meristem during leaf development in Arabidopsis thaliana L.

Planta ◽

10.1007/bf00197541 ◽

1994 ◽

Vol 194 (3) ◽

Cited By ~ 51

Author(s):

Makoto Fujie ◽

Haruko Kuroiwa ◽

Shigeyuki Kawano ◽

Shoshi Mutoh ◽

Tsuneyoshi Kuroiwa

Keyword(s):

Arabidopsis Thaliana ◽

Shoot Apical Meristem ◽

Leaf Development ◽

Apical Meristem

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Quantitative analysis of auxin sensing in leaf primordia argues against proposed role in regulating leaf dorsoventrality

eLife ◽

10.7554/elife.39298 ◽

2019 ◽

Vol 8 ◽

Cited By ~ 11

Author(s):

Neha Bhatia ◽

Henrik Åhl ◽

Henrik Jönsson ◽

Marcus G Heisler

Keyword(s):

Quantitative Analysis ◽

Developmental Stage ◽

Shoot Apical Meristem ◽

Leaf Development ◽

Apical Meristem ◽

Leaf Primordia ◽

Leaf Polarity

Dorsoventrality in leaves has been shown to depend on the pre-patterned expression of KANADI and HD-ZIPIII genes within the plant shoot apical meristem (SAM). However, it has also been proposed that asymmetric auxin levels within initiating leaves help establish leaf polarity, based in part on observations of the DII auxin sensor. By analyzing and quantifying the expression of the R2D2 auxin sensor, we find that there is no obvious asymmetry in auxin levels during Arabidopsis leaf development. We further show that the mDII control sensor also exhibits an asymmetry in expression in developing leaf primordia early on, while it becomes more symmetric at a later developmental stage as reported previously. Together with other recent findings, our results argue against the importance of auxin asymmetry in establishing leaf polarity.

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An integrated analysis of cell-type specific gene expression reveals genes regulated by REVOLUTA and KANADI1 in the Arabidopsis shoot apical meristem

PLoS Genetics ◽

10.1371/journal.pgen.1008661 ◽

2020 ◽

Vol 16 (4) ◽

pp. e1008661 ◽

Cited By ~ 2

Author(s):

Hasthi Ram ◽

Sudeep Sahadevan ◽

Nittaya Gale ◽

Monica Pia Caggiano ◽

Xiulian Yu ◽

...

Keyword(s):

Gene Expression ◽

Shoot Apical Meristem ◽

Apical Meristem ◽

Integrated Analysis ◽

Specific Gene ◽

Cell Type ◽

Specific Gene Expression ◽

Cell Type Specific

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Sample preparation for laser-microdissection of soybean shoot apical meristem

International Journal of Plant Biology ◽

10.4081/pb.2012.e3 ◽

2012 ◽

Vol 3 (1) ◽

pp. 3 ◽

Cited By ~ 1

Author(s):

Chui E. Wong ◽

Mohan B. Singh ◽

Prem L. Bhalla

Keyword(s):

Sample Preparation ◽

Shoot Apical Meristem ◽

Apical Meristem ◽

Laser Microdissection ◽

Specific Cell ◽

Legume Crop ◽

Continuous Formation ◽

Molecular Events ◽

Depth Analysis ◽

Specialized Equipment

The shoot apical meristem houses stem cells responsible for the continuous formation of aerial plant organs including leaves and stems throughout the life of plants. Laser-microdissection in combination with high-throughput technology such as next generation sequencing permits an in-depth analysis of molecular events associated with specific cell type of interest. Sample preparation is the most critical step in ensuring good quality RNA to be extracted from samples following laser-microdissection. Here, we optimized the sample preparation for a major legume crop, soybean. We used Farmer’s solution as a fixative and paraffin as the embedding medium for soybean shoot apical meristem tissue without the use of any specialized equipment. Shorter time for tissue fixation (two days) was found to be critical for the preservation of RNA in soybean shoot apical meristem. We further demonstrated the utility of this method for different tissues derived from soybean and rice. The method outlined here shall facilitate studies on crop plants involving laser-microdissection.

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