Multiple signal transduction pathways regulate discoidin I gene expression in Dictyostelium discoideum

Abstract We recently showed that mRNA levels coding the high-affinity Fc gamma receptor for IgG (Fc gamma R-I, CD64) and two of the components of the phagocytic superoxide anion-generating system--the heavy-chain subunit of cytochrome b558 (gp91-phox) and the 47-Kd cytosolic factor (p47- phox)--are modulated by interferon gamma (IFN-gamma). In this study, we examined whether dexamethasone (DEX) affects gp91-phox and p47-phox mRNA expression of human polymorphonuclear leukocytes (PMN), treated or not with IFN-gamma. We also investigated whether staurosporine, a general inhibitor of protein kinases, influences gp91-phox, p47-phox, and Fc gamma R-I gene expression in PMN treated with or without IFN- gamma. We found that (1) gp91-phox mRNA steady-state levels, expressed in control or IFN-gamma-treated PMN, were significantly inhibited, in a dose-dependent fashion, by both DEX and staurosporine; (2) p47-phox mRNA steady-state levels, expressed in control or IFN-gamma-treated PMN, were not influenced by DEX, but were markedly depressed by staurosporine; (3) no changes of spectrophotometric cytochrome b558 were found in PMN treated for up to 20 hours with the inhibitors, regardless of the presence of IFN-gamma; (4) both DEX and staurosporine dose-dependently inhibited IFN-gamma-induced Fc gamma R-I mRNA and protein expression; and (5) stability of gp91-phox and Fc gamma R-I messages in IFN-gamma-treated PMN was not altered by the presence of DEX. Our results demonstrate that gp91-phox, p47-phox, and Fc gamma R-I gene expression of PMN is governed by specific and independent biochemical pathways. Moreover, IFN-gamma activates different signal transduction pathways to modulate mRNA expression of gp91-phox, p47- phox, and Fc gamma R-I.

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Echinacea alkylamides modulate TNF-α gene expression via cannabinoid receptor CB2 and multiple signal transduction pathways

FEBS Letters ◽

10.1016/j.febslet.2004.10.064 ◽

2004 ◽

Vol 577 (3) ◽

pp. 563-569 ◽

Cited By ~ 109

Author(s):

Juerg Gertsch ◽

Roland Schoop ◽

Urs Kuenzle ◽

Andy Suter

Keyword(s):

Gene Expression ◽

Signal Transduction ◽

Cannabinoid Receptor ◽

Signal Transduction Pathways ◽

Multiple Signal ◽

Tnf Α

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Evidence for the involvement of distinct signal transduction pathways in the regulation of constitutive and interferon gamma-dependent gene expression of NADPH oxidase components (gp91-phox, p47-phox, and p22- phox) and high-affinity receptor for IgG (Fc gamma R-I) in human polymorphonuclear leukocytes

Blood ◽

10.1182/blood.v79.3.735.bloodjournal793735 ◽

1992 ◽

Vol 79 (3) ◽

pp. 735-744

Author(s):

MA Amezaga ◽

F Bazzoni ◽

C Sorio ◽

F Rossi ◽

MA Cassatella

Keyword(s):

Gene Expression ◽

Signal Transduction ◽

Steady State ◽

Polymorphonuclear Leukocytes ◽

Signal Transduction Pathways ◽

Ifn Gamma ◽

High Affinity ◽

Steady State Levels ◽

Human Polymorphonuclear Leukocytes ◽

I Gene

We recently showed that mRNA levels coding the high-affinity Fc gamma receptor for IgG (Fc gamma R-I, CD64) and two of the components of the phagocytic superoxide anion-generating system--the heavy-chain subunit of cytochrome b558 (gp91-phox) and the 47-Kd cytosolic factor (p47- phox)--are modulated by interferon gamma (IFN-gamma). In this study, we examined whether dexamethasone (DEX) affects gp91-phox and p47-phox mRNA expression of human polymorphonuclear leukocytes (PMN), treated or not with IFN-gamma. We also investigated whether staurosporine, a general inhibitor of protein kinases, influences gp91-phox, p47-phox, and Fc gamma R-I gene expression in PMN treated with or without IFN- gamma. We found that (1) gp91-phox mRNA steady-state levels, expressed in control or IFN-gamma-treated PMN, were significantly inhibited, in a dose-dependent fashion, by both DEX and staurosporine; (2) p47-phox mRNA steady-state levels, expressed in control or IFN-gamma-treated PMN, were not influenced by DEX, but were markedly depressed by staurosporine; (3) no changes of spectrophotometric cytochrome b558 were found in PMN treated for up to 20 hours with the inhibitors, regardless of the presence of IFN-gamma; (4) both DEX and staurosporine dose-dependently inhibited IFN-gamma-induced Fc gamma R-I mRNA and protein expression; and (5) stability of gp91-phox and Fc gamma R-I messages in IFN-gamma-treated PMN was not altered by the presence of DEX. Our results demonstrate that gp91-phox, p47-phox, and Fc gamma R-I gene expression of PMN is governed by specific and independent biochemical pathways. Moreover, IFN-gamma activates different signal transduction pathways to modulate mRNA expression of gp91-phox, p47- phox, and Fc gamma R-I.

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TNF-α and IFN-γ-mediated signal transduction pathways: Effects on IRF-1 and MHC class I gene expression in oligodendrocytes

Immunology Letters ◽

10.1016/s0165-2478(97)88903-1 ◽

1997 ◽

Vol 56 ◽

pp. 465

Author(s):

C. Agresti ◽

A. Bernardo ◽

N. Del Russo ◽

G. Marziali ◽

A. Battistini ◽

...

Keyword(s):

Gene Expression ◽

Signal Transduction ◽

Mhc Class I ◽

Class I ◽

Signal Transduction Pathways ◽

Tnf Α ◽

Ifn Γ ◽

I Gene ◽

Mhc Class I Gene

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Multiple signal transduction pathways regulate clusterin (gp 80) gene expression in MDCK cells

Journal of Molecular Endocrinology ◽

10.1677/jme.0.0170109 ◽

1996 ◽

Vol 17 (2) ◽

pp. 109-119 ◽

Cited By ~ 7

Author(s):

C Gutacker ◽

R Flach ◽

P Diel ◽

G Klock ◽

C Koch-Brandt

Keyword(s):

Gene Expression ◽

Signal Transduction ◽

Tissue Injury ◽

Mdck Cells ◽

Signal Transduction Pathways ◽

Epithelial Cell Line ◽

Multiple Signal ◽

Single Cell Type ◽

Induced Apoptosis ◽

The Individual

ABSTRACT Clusterin (gp 80, apolipoprotein J, TRPM-2) is a widely expressed multifunctional glycoprotein. Its demonstrated and proposed functions include the transport of lipids and membrane fragments, the inhibition of the cytolytic action of the terminal complement complex and the modulation of cell—cell interactions. The expression of the gene is enhanced during tissue injury and remodelling and by hormone-withdrawal-induced apoptosis of prostate and mammary cells. We show here that, in the kidney-derived epithelial cell line MDCK, clusterin mRNA is repressed by glucocorticoids and by progesterone. Treatment with epidermal growth factor also represses clusterin gene expression in MDCK cells. Incubation with 12-O-tetradecanoyl-phorbol-13-acetate, which activates protein kinase C (PKC), induces clusterin mRNA, while chelerythrine, an inhibitor of PKC, represses clusterin gene expression, suggesting that the clusterin gene responds to signalling pathways involving PKC. These results open up the possibility of studying the complex regulation of the clusterin gene by multiple signal transduction pathways within a single cell type, and most importantly, of characterizing interactions between the individual signal transduction cascades.

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