Association of 5' CpG demethylation and altered chromatin structure in the promoter region with transcriptional activation of the multidrug resistance 1 gene in human cancer cells

1999 ◽  
Vol 262 (3) ◽  
pp. 924-932 ◽  
Author(s):  
Hitoshi Kusaba ◽  
Masaharu Nakayama ◽  
Taishi Harada ◽  
Minoru Nomoto ◽  
Kimitoshi Kohno ◽  
...  
Keyword(s):  
Multidrug Resistance ◽  
Cancer Cells ◽  
Chromatin Structure ◽  
Transcriptional Activation ◽  
Promoter Region ◽  
Human Cancer ◽  
Human Cancer Cells ◽  
Multidrug Resistance 1 Gene

scholarly journals Template activating factor-I epigenetically regulates the TERT transcription in human cancer cells

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kohsuke Kato ◽  
Atsushi Kawaguchi ◽  
Kyosuke Nagata
Keyword(s):  
Cancer Cells ◽  
Transcriptional Activation ◽  
Human Cancer ◽  
Tumor Development ◽  
Telomerase Activity ◽  
Transcription Start ◽  
Cpg Dinucleotides ◽  
Human Cancer Cells ◽  
Factor I ◽  
Repeat Dna

AbstractTelomere, the terminus of linear chromosome in eukaryotes, is composed of specific repeat DNA which is mainly synthesized by a protein complex called telomerase. The maintenance of telomere DNA is important for unlimited proliferative capacity of cancer cells. The telomerase activity is controlled by the expression level of telomerase reverse transcriptase (TERT), a catalytic unit of telomerase, in some species including human. Therefore, to reveal the regulatory mechanisms of the transcription of TERT gene is important for understanding the tumor development. We found that template activating factor-I (TAF-I), a multifunctional nuclear protein, is involved in the transcriptional activation of TERT for the maintenance of telomere DNA in HeLa cells. TAF-I maintains the histone H3 modifications involved in transcriptional activation and hypomethylated cytosines in CpG dinucleotides around the transcription start site (TSS) in the TERT gene locus. Collectively, TAF-I is involved in the maintenance of telomere DNA through the regulation of TERT transcription, then consequently the occurrence and/or recurrence of cancer cells.

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