EFFECT OF PROPRANOLOL ON CENTRAL NEUROTRANSMITTER RELEASE IN WISTAR RATS ANALYSED BY BRAIN MICRODIALYSIS

Neurotransmitter release is mediated by the fast, calcium-triggered fusion of synaptic vesicles with the presynaptic plasma membrane, followed by endocytosis and recycling of the membrane of synaptic vesicles. While many of the proteins governing these processes are known, their regulation is only beginning to be understood. Here we have applied quantitative phosphoproteomics to identify changes in phosphorylation status of presynaptic proteins in resting and stimulated nerve terminals isolated from the brains of Wistar rats. Using rigorous quantification, we identified 252 phosphosites that are either up- or downregulated upon triggering calcium-dependent exocytosis. Particularly pronounced were regulated changes of phosphosites within protein constituents of the presynaptic active zone, including bassoon, piccolo, and RIM1. Additionally, we have mapped kinases and phosphatases that are activated upon stimulation. Overall, our study provides a snapshot of phosphorylation changes associated with presynaptic activity and provides a foundation for further functional analysis of key phosphosites involved in presynaptic plasticity.

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Ultrastructural Investigation of Spontaneous Pituitary Adenomas in Aging Sprague-Dawley Rats

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100053747 ◽

1982 ◽

Vol 40 ◽

pp. 216-217

Author(s):

D. J. McComb ◽

J. Beri ◽

F. Zak ◽

K. Kovacs

Keyword(s):

Microscopic Study ◽

Pituitary Adenomas ◽

Wistar Rats ◽

Microscopic Level ◽

Sprague Dawley ◽

Prolactin Cells ◽

Light Microscopic Level ◽

Ultrastructural Investigation ◽

Sprague Dawley Rats ◽

Long Evans

Investigation of the spontaneous pituitary adenomas in rat have been limited mainly to light microscopic study. Furth et al. (1973) described them as chromophobic, secreting prolactin. Kovacs et al. (1977) in an ul trastructural investigation of adenomas of old female Long-Evans rats, found that they were composed of prolactin cells. Berkvens et al. (1980) using immunocytochemistry at the light microscopic level, demonstrated that some spontaneous tumors of old Wistar rats could contain GH, TSH or ACTH as well as PRL.

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Light and electron microscopic investigation of adenohypophyses of germfree, food-restricted, and germfree-food restricted aging, male lobund-wistar rats

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100103826 ◽

1988 ◽

Vol 46 ◽

pp. 352-353

Author(s):

G. Ilse ◽

K. Kovacs ◽

N. Ryan ◽

T. Sano ◽

L. Stefaneanu ◽

...

Keyword(s):

Wistar Rats ◽

Microscopic Investigation ◽

Electron Microscopic Investigation ◽

Aging Male ◽

Sprague Dawley ◽

Electron Microscopic ◽

Sprague Dawley Rats ◽

Old Rats ◽

Ad Libitum ◽

Microscopic Findings

Germfree state and food restriction have been shown to increase life span and delay tumor occurrence in rats. We report here the histologic, immunocytochemical and electron microscopic findings of adenohypophyses of aging, male Lobund-Wistar rats raised at Lobund Laboratories. In our previous study, the morphologic changes in the adenohypophyses of old rats have been extensively investigated by histology, immunocytochemistry and electron microscopy. Lactotroph adenomas were frequent in Long-Evans and Sprague-Dawley rats, whereas gonadotroph adenomas were frequent in Sprague-Dawley and Wistar rats.Male Lobund-Wistar rats were divided into four groups: 1) conventional, which were raised under normal non-germfree environment and received food ad libitum; 2) germfree-food ad libitum; 3) conventional environment-food restricted and 4) germfree-food restricted. The adenohypophyses were removed from 6-month-, 18-month- and 30-month-old rats. For light microscopy, adenohypophyses were fixed in formalin and embedded in paraffin.

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Surface epithelial ultrastructure of the polycystic ovary in the BB wistar rat

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100142888 ◽

1986 ◽

Vol 44 ◽

pp. 252-253

Author(s):

R. C. Kaufmann ◽

F. K. Khosho ◽

K. S. Amankwah

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Wistar Rats ◽

Polycystic Ovary ◽

Wistar Rat ◽

Polycystic Ovaries ◽

Chemical Diabetes ◽

Scanning Electron

Diabetes decreases the fertility of females, but the mechanisms are not completely understood. In our investigations, we have found that 13% of the female BB Wistar rats that spontaneously developed chemical diabetes had persistent estrous. In this study the ovaries of these rats were examined by scanning electron microscopy(SEM) and compared to normal-cycling controls as well as to rats that had developed polycystic ovaries(PCO) by exposure to constant 1ight.

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Structural and functional renal alterations in five-sixth nephrectomized rats on unrestricted diet

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s042482010014333x ◽

1986 ◽

Vol 44 ◽

pp. 342-343

Author(s):

I. Stachura ◽

M. Pardo ◽

J. Costello ◽

D.M. Landwehr

Keyword(s):

Wistar Rats ◽

Renal Damage ◽

Standard Diet ◽

Experimental Conditions ◽

Phosphate Intake ◽

Dietary Restrictions ◽

Severe Reduction ◽

Male Wistar Rats ◽

Unrestricted Diet ◽

Progressive Renal Insufficiency

Under experimental conditions severe reduction of renal mass results in the hyperfiltration of the remaining nephrons leading to a progressive renal insufficiency. Similar changes are observed in patients with various renal disorders associated with a loss of the functioning nephrons. The progression of renal damage is accelerated by high protein and phosphate intake, and may be modified by the dietary restrictions.We studied 50 five-sixth nephrectarrized male Wistar rats on a standard diet (Rodent Laboratory Chow 5001 Ralston Purina Co., Richmond, Indiana; containing 23.4% protein) over a 20 week period.

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