scholarly journals Comparative analysis of denaturing gradient gel electrophoresis and temporal temperature gradient gel electrophoresis in separating Escherichia coli uidA amplicons differing in single base substitutions

2000 ◽  
Vol 30 (6) ◽  
pp. 427-431 ◽  
Author(s):  
A.H. Farnleitner ◽  
N. Kreuzinger ◽  
G.G. Kavka ◽  
S. Grillenberger ◽  
J. Rath ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Comparative Analysis ◽  
Temperature Gradient ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Single Base ◽  
Base Substitutions ◽  
Gradient Gel Electrophoresis ◽  
Temperature Gradient Gel Electrophoresis

scholarly journals Application of temperature gradient gel electrophoresis to the characterization of a nitrifying bioaugmentation product

2003 ◽  
Vol 43 (2) ◽  
pp. 277-286 ◽  
Author(s):  
Melissa A. Fouratt ◽  
Jeremy S. Rhodes ◽  
Charles M. Smithers ◽  
Nancy G. Love ◽  
Ann M. Stevens
Keyword(s):  
Temperature Gradient ◽  
Gel Electrophoresis ◽  
Gradient Gel Electrophoresis ◽  
Temperature Gradient Gel Electrophoresis

scholarly journals Denaturing gradient gel electrophoresis as a fingerprinting method for the analysis of soil microbial communities

2009 ◽  
Vol 55 (No. 10) ◽  
pp. 413-423 ◽  
Author(s):  
V. Valášková ◽  
P. Baldrian
Keyword(s):  
Microbial Communities ◽  
Gel Electrophoresis ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Soil Microbial Communities ◽  
Soil Microbial ◽  
Genes Encoding ◽  
Gradient Gel Electrophoresis ◽  
Temperature Gradient Gel Electrophoresis ◽  
Experimental Treatments ◽  
Multiple Samples

In soil microbial ecology, the effects of environmental factors and their gradients, temporal changes or the response to specific experimental treatments of microbial communities can only be effectively analyzed using methods that address the structural differences among whole communities. Fingerprinting methods are the most appropriate technique for this task when multiple samples must be analyzed. Among the methods currently used to compare microbial communities based on nucleic acid sequences, the techniques based on differences in the melting properties of double-stranded molecules, denaturing gradient gel electrophoresis (DGGE) or temperature gradient gel electrophoresis (TGGE), are the most widely used. Their main advantage is that they provide the possibility to further analyze whole sequences contained in fingerprints using molecular methods. In addition to the analysis of microbial communities based on DNA extracted from soils, DGGE/TGGE can also be used for the assessment of the active part of the community based on the analysis of RNA-derived sequences or for the analysis of sequences of functional genes encoding for proteins involved in important soil processes.

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