Oviposition bioassay responses of Culex tarsalis and Culex quinquefasciatus to neem products containing azadirachtin

1999 ◽  
Vol 91 (2) ◽  
pp. 337-345 ◽  
Author(s):  
Tianyun Su ◽  
Mir S. Mulla
Viruses ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 226
Author(s):  
Bethany L. McGregor ◽  
C. Roxanne Connelly ◽  
Joan L. Kenney

Oropouche virus (OROV), a vector-borne Orthobunyavirus circulating in South and Central America, causes a febrile illness with high rates of morbidity but with no documented fatalities. Oropouche virus is transmitted by numerous vectors, including multiple genera of mosquitoes and Culicoides biting midges in South America. This study investigated the vector competence of three North American vectors, Culex tarsalis, Culex quinquefasciatus, and Culicoides sonorensis, for OROV. Cohorts of each species were fed an infectious blood meal containing 6.5 log10 PFU/mL OROV and incubated for 10 or 14 days. Culex tarsalis demonstrated infection (3.13%) but not dissemination or transmission potential at 10 days post infection (DPI). At 10 and 14 DPI, Cx. quinquefasciatus demonstrated 9.71% and 19.3% infection, 2.91% and 1.23% dissemination, and 0.97% and 0.82% transmission potential, respectively. Culicoides sonorensis demonstrated 86.63% infection, 83.14% dissemination, and 19.77% transmission potential at 14 DPI. Based on these data, Cx. tarsalis is unlikely to be a competent vector for OROV. Culex quinquefasciatus demonstrated infection, dissemination, and transmission potential, although at relatively low rates. Culicoides sonorensis demonstrated high infection and dissemination but may have a salivary gland barrier to the virus. These data have implications for the spread of OROV in the event of a North American introduction.


1943 ◽  
Vol 78 (4) ◽  
pp. 241-253 ◽  
Author(s):  
W. McD. Hammon ◽  
W. C. Reeves

1. St. Louis virus has been successfully transmitted in the laboratory by the following 9 species of mosquitoes from 3 genera: Culex tarsalis, Culex pipiens, Culex coronator, Aedes lateralis, Aedes taeniorhynchus, Aedes vexans, Aedes nigromaculis, Theobaldia incidens, and Theobaldia inornata. 2. Though transmission has not been demonstrated, survival of the virus for more than a few days was shown to occur in Culex quinquefasciatus, Culex stigmatosoma, Psorophora ciliata, and Anopheles maculipennis freeborni. 3. In experiments with Culex tarsalis, infection occurred from feeding on chickens and ducks which had been previously inoculated by the subcutaneous route. After an incubation period these mosquitoes infected other chickens and virus was in turn demonstrated in the blood of these. This is interpreted as proof that fowl may serve as reservoirs of virus in nature. Since mosquitoes have been repeatedly found naturally infected with St. Louis virus and epidemiologic evidence supports their incrimination, their rôle as vectors is now established. The fully incriminated species is Culex tarsalis.


2018 ◽  
Vol 12 (6) ◽  
pp. e0006524 ◽  
Author(s):  
Bradley J. Main ◽  
Jay Nicholson ◽  
Olivia C. Winokur ◽  
Cody Steiner ◽  
Kasen K. Riemersma ◽  
...  

2020 ◽  
Author(s):  
Bradley J. Main ◽  
Matteo Marcantonio ◽  
J. Spencer Johnston ◽  
Jason L. Rasgon ◽  
C. Titus Brown ◽  
...  

AbstractThe mosquito, Culex tarsalis, is a key vector in the western United States due to its role in transmission of zoonotic arboviruses that affect human health. Extensive research has been conducted on Cx. tarsalis ecology, feeding behavior, vector competence, autogeny, diapause, genetics, and insecticide resistance. Population genetic analyses in the western U.S. have identified at least three genetic clusters that are geographically distinct. Salivary gland-specific gene expression has also revealed genes involved in blood feeding. However, genetic studies of this mosquito have been hindered by the lack of a reference genome. To facilitate genomic studies in Cx. tarsalis, we have assembled and annotated a reference genome (CtarK1) based on PacBio HiFi reads from a single male. Using the Cx. tarsalis transcriptome and protein sequences from Culex quinquefasciatus, approximately 17,456 protein-coding genes, including the para insecticide resistance gene, were annotated in the CtarK1 genome. Genome completeness was assessed using the Benchmarking Universal Single-Copy Orthologs (BUSCO) tool, which identified 84.8% of the 2799 Dipteran BUSCO genes. The CtarK1 assembly is 790Mb with an N50 of 58kb. Using full mitochondrial genome alignments with other sequenced mosquito genomes we present a Bayesian phylogeny, which estimates that the divergence of Cx. tarsalis from Culex quinquefasciatus, the most closely related mosquito species with a genome, occurred 15.8-22.2 million years ago.


2008 ◽  
Vol 4 (7) ◽  
pp. 74-78
Author(s):  
Martha Giselle Rivera Pineda ◽  
Sandra Patricia Garzón Lozano ◽  
Luz Inés Villarreal Salazar

El mosquito Culex quinquefasciatus ha surgido como una plaga urbana de importancia sanitaria en Bogotá y sus alrededores. Su presencia es favorecida por la proliferación de una planta acuática, el buchón, cuyo crecimiento es estimulado por vertimientos de aguas residuales en ríos y embalses. Dentro de los diferentes tipos de control posibles de este insecto plaga, se destaca el control biológico, el cual presenta ventajas en cuanto a su eficiencia y bajo impacto ambiental.


2009 ◽  
Vol 5 (9) ◽  
pp. 25-38
Author(s):  
Martha Giselle Rivera Pineda ◽  
Sandra Patricia Garzón Lozano ◽  
Luz Inés Villarreal Salazar

En busca de una alternativa de control del mosquito Culex quinquefasciatus en áreas urbanas se desarrolló el proyecto: “Evaluación de microorganismos biocontroladores sobre el mosquito Culex quinquefasciatus” cuyo objetivo fue evaluar la capacidad biocontroladora de diferentes hongos entomopatógenos sobre larvas del mosquito a nivel de laboratorio. Se evaluaron 16 aislamientos de hongos en diferentes concentraciones. Se constataron diferencias en el porcentaje de mortalidad producido en larvas de Cx. quinquefasciatus dependientes del tipo de aislamiento, de la concentración utilizada y del tiempo de exposición. Los aislamientos que presentaron el nivel de mortalidad más alto pertenecen a la especie Metarhizium anisopliae, seguidos por aislamientos de la especie Beauveria bassiana. El aislamiento que presentó el mayor nivel de mortalidad (100%) en menor tiempo de exposición (24 horas) fue Metarhizium anisopliae 99068 en una concentración de 1x106 e/ml. Por lo tanto este aislamiento es recomendado para su evaluación en campo y desarrollo de un producto comercial.


2009 ◽  
Vol 24 ◽  
pp. 103-112
Author(s):  
Vaijayanti Devi Neupane ◽  
Ishan Gautam ◽  
A. S. Tamrakar ◽  
Shambhu Raj Shrestha

A study was carried out at 10 villages of Chitwan District of Nepal during August-September, 1997 to March- April, 1998. A total of 12 species among 1197 samples were collected. Culex quinquefasciatus, Cx. fuscocephala, Cx. tritaeniorhynchus, Cx. gelidus, Cx. thelieri were previously reported, vector of different mosquito born diseases were recorded. Cx. quinquefasciatus was the most abundant both in post monsoon (26.90%) and pre monsoon (18.7%). Highest resting density (Human) of 9.33 was recorded in Post Monsoon at 25.75º C in Champanagar. Indoor resting density (Cattle) was highest in Suryapur at 22º C in Post Monsoon. The highest outdoor density (0.22) was at 22.5º C in Kholesimal in Pre Monsoon. Resting habit of Cx. tritaeniorhynchus & Cx. mimulus was infinitive in Post Monsoon.  Key Words:  Culex; JE Filariasis;  Abundant;  Resting habit; Chitwan. Journal of Natural History Museum Vol. 24, 2009,Page: 103-113 


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