Energy losses: aspects not considered and the use of probabilistic methods

2009 ◽  
Author(s):  
P.R.F. de M. Bastos ◽  
N. Ferreira ◽  
B.A. de Souza
Author(s):  
Willem H.J. Andersen

Electron microscope design, and particularly the design of the imaging system, has reached a high degree of perfection. Present objective lenses perform up to their theoretical limit, while the whole imaging system, consisting of three or four lenses, provides very wide ranges of magnification and diffraction camera length with virtually no distortion of the image. Evolution of the electron microscope in to a routine research tool in which objects of steadily increasing thickness are investigated, has made it necessary for the designer to pay special attention to the chromatic aberrations of the magnification system (as distinct from the chromatic aberration of the objective lens). These chromatic aberrations cause edge un-sharpness of the image due to electrons which have suffered energy losses in the object.There exist two kinds of chromatic aberration of the magnification system; the chromatic change of magnification, characterized by the coefficient Cm, and the chromatic change of rotation given by Cp.


Author(s):  
C.L. Woodcock ◽  
R.A. Horowitz ◽  
D. P. Bazett-Jones ◽  
A.L. Olins

In the eukaryotic nucleus, DNA is packaged into nucleosomes, and the nucleosome chain folded into ‘30nm’ chromatin fibers. A number of different model structures, each with a specific location of nucleosomal and linker DNA have been proposed for the arrangment of nucleosomes within the fiber. We are exploring two strategies for testing the models by localizing DNA within chromatin: electron spectroscopic imaging (ESI) of phosphorus atoms, and osmium ammine (OSAM) staining, a method based on the DNA-specific Feulgen reaction.Sperm were obtained from Patiria miniata (starfish), fixed in 2% GA in 150mM NaCl, 15mM HEPES pH 8.0, and embedded In Lowiciyl K11M at -55C. For OSAM staining, sections 100nm to 150nm thick were treated as described, and stereo pairs recorded at 40,000x and 100KV using a Philips CM10 TEM. (The new osmium ammine-B stain is available from Polysciences Inc). Uranyl-lead (U-Pb) staining was as described. ESI was carried out on unstained, very thin (<30 nm) beveled sections at 80KV using a Zeiss EM902. Images were recorded at 20,000x and 30,000x with median energy losses of 110eV, 120eV and 160eV, and a window of 20eV.


2017 ◽  
Vol 95 (5) ◽  
pp. 2133
Author(s):  
A. L. Shreck ◽  
P. J. Ebert ◽  
E. A. Bailey ◽  
J. S. Jennings ◽  
K. D. Casey ◽  
...  

2019 ◽  
pp. 90-95
Author(s):  
V. A. Minaev ◽  
I. D. Korolev ◽  
O. A. Kulish ◽  
A. V. Mazin

The existing methods of information delivery to the strategic and tactical management of many government agencies are expensive, not always reliable and efficient. Therefore, quantum cryptographic systems (QCS) have been actively developed in recent years. However, there are problems with the use of the QCS associated with the reliability of information transfer. First, the existing fiber-optic communication channels (FOCC) are not designed to transmit single-photon signals, which leads to the complexity of their cryptographic protection. The second is insufficiently methodically developed calculation of energy losses and errors in the evaluation of the characteristics of information transfer in FOCC QCS. In article the analysis of the energy loss factors in the classical fiber-optic channel is carried out and the additive loss formula is discussed in detail. Then we consider the fiber-optic channel of quantum information transmission with the use of integrated optical devices. The additive formula of optical losses in such a channel is discussed. The features of losses in integrated optical devices are shown. The features of quantum cryptographic system of information transmission are considered. As a result, the model of FOCC QCS taking into account energy losses is presented, which allows competently in theoretical terms and visualize the passage of information through modern quantum cryptographically secure telecommunications while providing control in government structures.


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