scholarly journals Study on the determination of felodipine sample content by fluorescence method and its application

2021 ◽  
Vol 257 ◽  
pp. 02094
Author(s):  
Yue Zhang ◽  
Jiang Wu ◽  
Fenghua Liu ◽  
Chen Wang ◽  
Yuqing Cheng ◽  
...  
Keyword(s):  
Reliable Method ◽  
Fluorescence Method ◽  
Fluorescence Properties ◽  
Actual Sample ◽  
Test Result

This experiment researched and constructed a complex with good fluorescence properties, which can be used to detect the content of felodipine. It provides a new and reliable method for the determination of felodipine. The actual sample of felodipine was successfully tested, and the test result was compared with the labeled content. It was proved that the content determined in this experiment was basically consistent with the standard content in the sample, and the reproducibility was good. Therefore, this method can be used to determine the content of felodipine in drugs, and even the content of felodipine in different substances.

The Need for Using An Enzymatic Colorimetric Assay in Creatinine Determination of Peritoneal Dialysis Solutions

1990 ◽  
Vol 10 (1) ◽  
pp. 89-92 ◽  
Author(s):  
Liliane Larpent ◽  
Christian Verger
Keyword(s):  
Peritoneal Dialysis ◽  
Reliable Method ◽  
Colorimetric Assay ◽  
Colorimetric Method ◽  
Peritoneal Membrane ◽  
Creatinine Kinetics ◽  
Dialysis Solutions ◽  
Peritoneal Dialysis Solutions ◽  
Enzymatic Test

The fate of the peritoneal membrane on continuous ambulatory peritoneal dialysis (CAPD) is usually evaluated through the modification of its permeability to various solutes as glucose, creatinine, and urea. Therefore, the accuracy of the methods used for measurements of creatinine is of great importance. A particular problem does exist for creatinine determination as it may be influenced by the presence of glucose. We studied a new enzymatic colorimetric method for creatinine determination in peritoneal dialysis solutions which contain high dextrose concentrations. Creatinine was measured in plasma, urine, and dialysate from 18 patients on CAPD and in pure dextrose solutions, with an enzymatic test (Boehringer Mannheim) and with Jaffe's reaction on two different analyzers: Astra (Beckman) and Eris (Merck). Creatinine results were similar with both assays (Jaffe's reaction and enzymatic test) when measured in blood and urine. However the Jaffe's reaction gave higher creatinine results than the enzymatic test (p < 0.001), when assays were performed in peritoneal dialysis solutions and in pure glucose solutions. In addition, it appeared that other components of dialysis solutions, mainly calcium chloride, influenced unpredictably the results of creatinine with the Jaffe's reaction. We conclude that specific enzymatic test is a more accurate and reliable method to evaluate creatinine kinetics through the peritoneal membrane when determined in CAPD solutions.

Urinary gonadotropin measurements by enhanced luminometric assays (LIA) for the evaluation of pubertal development

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
And Demir ◽  
Adem Aydın ◽  
Atilla Büyükgebiz ◽  
Ulf-Håkan Stenman ◽  
Matti Hero
Keyword(s):  
Healthy Subjects ◽  
Reliable Method ◽  
Pubertal Development ◽  
High Sensitivity ◽  
Tanner Stage ◽  
Time Resolved ◽  
Stage 1 ◽  
Sensitive Detection Technique ◽  
Serum And Urine

Abstract Objectives Determination of LH in urine has proved to be a reliable method for evaluation of pubertal development. The human LH assay based on time-resolved immunofluorometric (IFMA) technology (AutoDELFIA, PerkinElmer, Wallac) has been found to be suitable for this purpose thanks to its high sensitivity but other assays have not been evaluated. We have analyzed our data obtained by another potentially sensitive detection technique, enhanced luminometric assay (LIA) with the objective of finding a viable alternative to IFMA since these may not be available in the future. Methods LIA was used to measure LH and FSH in serum and urine samples from 100 healthy subjects of each Tanner stage and both genders, whose pubertal development has been determined. Results Urinary gonodotropin concentrations measured by LIA correlated well with Tanner stage [(r=0.93 for girls, r=0.81 for boys; p<0.01 for LH) and (r=0.81 for girls, r=0.73 for boys; p<0.01 for FSH)]. LIA determinations revealed the increase in U-LH concentrations during the transition from Tanner stage 1–2 in both girls and boys (p<0.001), whereas U-FSH and S-LH were able to detect the increase from Tanner stage 1–2 only in boys or girls, respectively (both p<0.001). Conclusions Measurement of urinary gonadotropin concentrations by LIA may be useful for the evaluation of overall pubertal development and also in the detection of transition from prepuberty to puberty.

A simple and sensitive fluorescence method for the determination of trace ozone in air using acridine red as a probe

Luminescence ◽  
2014 ◽  
Vol 29 (8) ◽  
pp. 1102-1106 ◽  
Author(s):  
Qingye Liu ◽  
Chenyin Lin ◽  
Xinghui Zhang ◽  
Guiqing Wen ◽  
Aihui Liang
Keyword(s):  
Fluorescence Method ◽  
Acridine Red

Determination of memantine in rat plasma by HPLC-fluorescence method and its application to study of the pharmacokinetic interaction between memantine and methazolamide

2011 ◽  
Vol 26 (2) ◽  
pp. 214-219 ◽  
Author(s):  
Mohamed G. Hassan ◽  
Kamla M. Emara ◽  
Horria A. Mohamed ◽  
Hanaa M. Abdel-Wadood ◽  
Rie Ikeda ◽  
...  
Keyword(s):  
Pharmacokinetic Interaction ◽  
Rat Plasma ◽  
Fluorescence Method

Optimizationvia experimental design of an SPE-HPLC-UV-fluorescence method for the determination of valsartan and its metabolite in human plasma samples

2006 ◽  
Vol 29 (15) ◽  
pp. 2265-2283 ◽  
Author(s):  
Gorka Iriarte ◽  
Nerea Ferreirós ◽  
Izaskun Ibarrondo ◽  
Rosa Maria Alonso ◽  
Miren Itxaso Maguregi ◽  
...  
Keyword(s):  
Experimental Design ◽  
Human Plasma ◽  
Fluorescence Method ◽  
Plasma Samples ◽  
Uv Fluorescence ◽  
Human Plasma Samples

Determination of neutralization point for allergic hypersensitivity

1987 ◽  
Vol 76 (04) ◽  
pp. 230-234 ◽  
Author(s):  
Anthony D. Fox
Keyword(s):  
Reliable Method ◽  
Needle Puncture

AbstractThe electronic Vegatest method of determining environmental hypersensitivities (allergies) is fast, accurate, and does not require the use of any needle puncture of the skin of the patient. It provides a relatively reliable method of determining the neutralization point and is well suited to the use of homœopathic medicines.

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