scholarly journals Live-cell nanoscopy enabled with transient labeling and the control of fluorophore blinking

2018 ◽  
Vol 190 ◽  
pp. 03008
Author(s):  
Alexander Mishin ◽  
Maxim Perfilov ◽  
Alexey Gavrikov ◽  
Anastasia Mamontova ◽  
Alexey Bogdanov ◽  
...  
Keyword(s):  
Super Resolution ◽  
Live Cell ◽  
Dynamic Replacement ◽  
Fluorescent Tags

Live-cell super-resolution of proteins labeled with genetically encoded fluorescent tags is a challenging task because of the imperfect labeling and the inevitable deterioration of the signal in the course of the experiment. Incomplete maturation of the covalently attached fluorescent tags, inefficient photoconversion, and photobleaching further complicate prolonged live-cell nanoscopy. We have implemented two strategies for lowering the photodamage: ensuring the dynamic replacement of damaged molecules and establishing conditions for the robust intrinsic blinking of the tags at lower illumination powers.

scholarly journals Bio-orthogonal Red and Far-Red Fluorogenic Probes for Wash-Free Live-Cell and Super-resolution Microscopy

2021 ◽  
Author(s):  
Philipp Werther ◽  
Klaus Yserentant ◽  
Felix Braun ◽  
Kristin Grußmayer ◽  
Vytautas Navikas ◽  
...  
Keyword(s):  
Super Resolution ◽  
Live Cell ◽  
Fluorogenic Probes ◽  
Super Resolution Microscopy

scholarly journals Ultrasmall, Bright, and Photostable Fluorescent Core–Shell Aluminosilicate Nanoparticles for Live‐Cell Optical Super‐Resolution Microscopy

2021 ◽  
Vol 33 (8) ◽  
pp. 2006829
Author(s):  
Jacob A. Erstling ◽  
Joshua A. Hinckley ◽  
Nirmalya Bag ◽  
Jessica Hersh ◽  
Grant B. Feuer ◽  
...  
Keyword(s):  
Super Resolution ◽  
Live Cell ◽  
Core Shell ◽  
Super Resolution Microscopy

Instant Live-Cell Super-Resolution Imaging of Cellular Structures by Nanoinjection of Fluorescent Probes

Nano Letters ◽  
2015 ◽  
Vol 15 (2) ◽  
pp. 1374-1381 ◽  
Author(s):  
Simon Hennig ◽  
Sebastian van de Linde ◽  
Martina Lummer ◽  
Matthias Simonis ◽  
Thomas Huser ◽  
...  
Keyword(s):  
Fluorescent Probes ◽  
Super Resolution ◽  
Live Cell ◽  
Cellular Structures ◽  
Resolution Imaging

Live-Cell Super-Resolution Imaging with Synthetic Fluorophores

2012 ◽  
Vol 63 (1) ◽  
pp. 519-540 ◽  
Author(s):  
Sebastian van de Linde ◽  
Mike Heilemann ◽  
Markus Sauer
Keyword(s):  
Super Resolution ◽  
Live Cell ◽  
Resolution Imaging

scholarly journals Dynamic Organization of Chromatin Domains Revealed by Super-Resolution Live-Cell Imaging

2017 ◽  
Vol 67 (2) ◽  
pp. 282-293.e7 ◽  
Author(s):  
Tadasu Nozaki ◽  
Ryosuke Imai ◽  
Mai Tanbo ◽  
Ryosuke Nagashima ◽  
Sachiko Tamura ◽  
...  
Keyword(s):  
Live Cell Imaging ◽  
Cell Imaging ◽  
Super Resolution ◽  
Live Cell ◽  
Chromatin Domains ◽  
Dynamic Organization

scholarly journals C. elegans neurons have functional dendritic spines

eLife ◽  
2019 ◽  
Vol 8 ◽  
Author(s):  
Andrea Cuentas-Condori ◽  
Ben Mulcahy ◽  
Siwei He ◽  
Sierra Palumbos ◽  
Mei Zhen ◽  
...  
Keyword(s):  
Motor Neurons ◽  
Dendritic Spines ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Super Resolution ◽  
Live Cell ◽  
Model Organisms ◽  
Spine Density ◽  
C Elegans ◽  
Spine Function

Dendritic spines are specialized postsynaptic structures that transduce presynaptic signals, are regulated by neural activity and correlated with learning and memory. Most studies of spine function have focused on the mammalian nervous system. However, spine-like protrusions have been reported in C. elegans (Philbrook et al., 2018), suggesting that the experimental advantages of smaller model organisms could be exploited to study the biology of dendritic spines. Here, we used super-resolution microscopy, electron microscopy, live-cell imaging and genetics to show that C. elegans motor neurons have functional dendritic spines that: (1) are structurally defined by a dynamic actin cytoskeleton; (2) appose presynaptic dense projections; (3) localize ER and ribosomes; (4) display calcium transients triggered by presynaptic activity and propagated by internal Ca++ stores; (5) respond to activity-dependent signals that regulate spine density. These studies provide a solid foundation for a new experimental paradigm that exploits the power of C. elegans genetics and live-cell imaging for fundamental studies of dendritic spine morphogenesis and function.

scholarly journals Malic Acid Carbon Dots: From Super-resolution Live-Cell Imaging to Highly Efficient Separation

ACS Nano ◽  
2018 ◽  
Vol 12 (6) ◽  
pp. 5741-5752 ◽  
Author(s):  
Bo Zhi ◽  
Yi Cui ◽  
Shengyang Wang ◽  
Benjamin P. Frank ◽  
Denise N. Williams ◽  
...  
Keyword(s):  
Malic Acid ◽  
Carbon Dots ◽  
Live Cell Imaging ◽  
Cell Imaging ◽  
Super Resolution ◽  
Live Cell ◽  
Efficient Separation ◽  
Highly Efficient
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