Use of the comet assay to measure DNA damage in cells exposed to photosensitizers and gamma radiation

1999 ◽  
Vol 96 (1) ◽  
pp. 143-146 ◽  
Author(s):  
J.-P. Pouget ◽  
J.-L. Ravanat ◽  
T. Douki ◽  
M.-J. Richard ◽  
J. Cadet
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Gamma Radiation ◽  
In Cells

scholarly journals Analysis of DNA damage in cells excreted in urine of cervical cancer patients using alkaline comet assay

2014 ◽  
Vol 7 (Suppl 1) ◽  
pp. P12
Author(s):  
Avani Patel ◽  
Mihir Shah ◽  
Pinaki Patel ◽  
Trupti Patel
Keyword(s):  
Cervical Cancer ◽  
Dna Damage ◽  
Comet Assay ◽  
Cancer Patients ◽  
Alkaline Comet Assay ◽  
In Cells

scholarly journals Effects of gamma-radiation on DNA damage in onion (Allium cepa L.) seedlings

2019 ◽  
Vol 489 (2) ◽  
pp. 199-204
Author(s):  
A. Ya. Bolsunovsky ◽  
D. V. Dementyev ◽  
T. S. Frolova ◽  
E. A. Trofimova ◽  
E. M. Iniatkina ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Gamma Radiation ◽  
Allium Cepa ◽  
Low Dose ◽  
Nuclear Dna ◽  
Biological Effects ◽  
Dose Range ◽  
Dna Breaks ◽  
Allium Test

The effect of -radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation ( 0,1 Gy). Dose dependence of DNA damage parameters showed nonlinear behavior: a linear section in the low-dose region (below 0,1 Gy) and a dose-independent plateau in the dose range between 1 and 5 Gy. Thus, the comet assay can be used to estimate the biological effects of low-dose gamma-radiation on Allium cepa seedlings.

DNA Damage Induced in Cells by γ and UVA Radiation As Measured by HPLC/GC−MS and HPLC−EC and Comet Assay

2000 ◽  
Vol 13 (7) ◽  
pp. 541-549 ◽  
Author(s):  
J.-P. Pouget ◽  
T. Douki ◽  
M.-J. Richard ◽  
J. Cadet
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Uva Radiation ◽  
In Cells

Detection of DNA Damage by the Alkaline Comet Assay after Exposure to Low-Dose Gamma Radiation

1998 ◽  
Vol 149 (4) ◽  
pp. 396 ◽  
Author(s):  
Robert S. Malyapa ◽  
Chen Bi ◽  
Eric W. Ahern ◽  
Joseph L. Roti Roti
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Gamma Radiation ◽  
Low Dose ◽  
Alkaline Comet Assay

Optimization and Standardization of the "Comet Assay" for Analyzing the Repair of DNA Damage in Cells

1999 ◽  
Vol 175 (7) ◽  
pp. 333-340 ◽  
Author(s):  
Thomas Bauch ◽  
Wilfried Böcker ◽  
Ulrike Mallek ◽  
Wolfgang-Ulrich Müller ◽  
Christian Streffer
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
In Cells

scholarly journals Evaluation of gamma radiation-induced DNA damage in Aedes aegypti using the comet assay

2017 ◽  
Vol 33 (12) ◽  
pp. 930-937 ◽  
Author(s):  
Vinaya Shetty ◽  
NJ Shetty ◽  
SR Ananthanarayana ◽  
SK Jha ◽  
RC Chaubey
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Aedes Aegypti ◽  
Gamma Radiation ◽  
Linear Increase ◽  
Comet Tail ◽  
Potential Biomarker ◽  
Radiation Induced ◽  
Extent Of Damage ◽  
Different Doses

The study was undertaken to evaluate gamma radiation-induced DNA damage in Aedes aegypti. The comet assay was employed to demonstrate the extent of DNA damage produced in adult male A. aegypti exposed to seven different doses of gamma radiation, ranging from 1 Gy to 50 Gy. DNA damage was measured as the percentage of comet tail DNA. A significant linear increase in DNA damage was observed in all samples; the extent of damage being proportional to the dose of gamma radiation the organism received, except in those treated with 1 Gy. The highest amount of DNA damage was noticed at 1 h postirradiation, which decreased gradually with time, that is, at 3, 6 and 12 h postirradiation. This may indicate repair of the damaged DNA and/or loss of heavily damaged cells as the postirradiation time increased. The comet assay serves as a sensitive and rapid technique to detect gamma radiation-induced DNA damage in A. aegypti. This could be used as a potential biomarker for environmental risk assessment.

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