Detection of DNA Damage Induced by Topoisomerase II Inhibitors, Gamma Radiation and Crosslinking Agents Using the Comet Assay

2009 ◽  
pp. 169-176 ◽  
Author(s):  
Lori A. Hazlehurst
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Gamma Radiation ◽  
Topoisomerase Ii ◽  
Topoisomerase Ii Inhibitors ◽  
Crosslinking Agents

Use of the comet assay to measure DNA damage in cells exposed to photosensitizers and gamma radiation

1999 ◽  
Vol 96 (1) ◽  
pp. 143-146 ◽  
Author(s):  
J.-P. Pouget ◽  
J.-L. Ravanat ◽  
T. Douki ◽  
M.-J. Richard ◽  
J. Cadet
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Gamma Radiation ◽  
In Cells

scholarly journals Effects of gamma-radiation on DNA damage in onion (Allium cepa L.) seedlings

2019 ◽  
Vol 489 (2) ◽  
pp. 199-204
Author(s):  
A. Ya. Bolsunovsky ◽  
D. V. Dementyev ◽  
T. S. Frolova ◽  
E. A. Trofimova ◽  
E. M. Iniatkina ◽  
...  
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Gamma Radiation ◽  
Allium Cepa ◽  
Low Dose ◽  
Nuclear Dna ◽  
Biological Effects ◽  
Dose Range ◽  
Dna Breaks ◽  
Allium Test

The effect of -radiation on the level of nuclear DNA damage in onion seedlings (Allium-test) was studied using the comet assay. DNA breaks were first found in cells of onion seedlings exposed to low-dose radiation ( 0,1 Gy). Dose dependence of DNA damage parameters showed nonlinear behavior: a linear section in the low-dose region (below 0,1 Gy) and a dose-independent plateau in the dose range between 1 and 5 Gy. Thus, the comet assay can be used to estimate the biological effects of low-dose gamma-radiation on Allium cepa seedlings.

scholarly journals Cell survival after DNA damage in the comet assay

2021 ◽  
Author(s):  
Ezgi Eyluel Bankoglu ◽  
Carolin Schuele ◽  
Helga Stopper
Keyword(s):  
Dna Damage ◽  
Cell Death ◽  
Comet Assay ◽  
Survival Data ◽  
Topoisomerase Ii ◽  
Basic Research ◽  
Human Biomonitoring ◽  
A Cell ◽  
Tk6 Cells ◽  
Genotoxicity Testing

AbstractThe comet assay is widely used in basic research, genotoxicity testing, and human biomonitoring. However, interpretation of the comet assay data might benefit from a better understanding of the future fate of a cell with DNA damage. DNA damage is in principle repairable, or if extensive, can lead to cell death. Here, we have correlated the maximally induced DNA damage with three test substances in TK6 cells with the survival of the cells. For this, we selected hydrogen peroxide (H2O2) as an oxidizing agent, methyl methanesulfonate (MMS) as an alkylating agent and etoposide as a topoisomerase II inhibitor. We measured cell viability, cell proliferation, apoptosis, and micronucleus frequency on the following day, in the same cell culture, which had been analyzed in the comet assay. After treatment, a concentration dependent increase in DNA damage and in the percentage of non-vital and apoptotic cells was found for each substance. Values greater than 20–30% DNA in tail caused the death of more than 50% of the cells, with etoposide causing slightly more cell death than H2O2 or MMS. Despite that, cells seemed to repair of at least some DNA damage within few hours after substance removal. Overall, the reduction of DNA damage over time is due to both DNA repair and death of heavily damaged cells. We recommend that in experiments with induction of DNA damage of more than 20% DNA in tail, survival data for the cells are provided.

Detection of DNA Damage by the Alkaline Comet Assay after Exposure to Low-Dose Gamma Radiation

1998 ◽  
Vol 149 (4) ◽  
pp. 396 ◽  
Author(s):  
Robert S. Malyapa ◽  
Chen Bi ◽  
Eric W. Ahern ◽  
Joseph L. Roti Roti
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Gamma Radiation ◽  
Low Dose ◽  
Alkaline Comet Assay

scholarly journals Molecular effects of topoisomerase II inhibitors in AML cell lines: correlation of apoptosis with topoisomerase II activity but not with DNA damage

Leukemia ◽  
1999 ◽  
Vol 13 (11) ◽  
pp. 1859-1863 ◽  
Author(s):  
F Gieseler ◽  
E Bauer ◽  
V Nuessler ◽  
M Clark ◽  
S Valsamas
Keyword(s):  
Dna Damage ◽  
Cell Lines ◽  
Topoisomerase Ii ◽  
Topoisomerase Ii Inhibitors ◽  
Molecular Effects

scholarly journals Evaluation of gamma radiation-induced DNA damage in Aedes aegypti using the comet assay

2017 ◽  
Vol 33 (12) ◽  
pp. 930-937 ◽  
Author(s):  
Vinaya Shetty ◽  
NJ Shetty ◽  
SR Ananthanarayana ◽  
SK Jha ◽  
RC Chaubey
Keyword(s):  
Dna Damage ◽  
Comet Assay ◽  
Aedes Aegypti ◽  
Gamma Radiation ◽  
Linear Increase ◽  
Comet Tail ◽  
Potential Biomarker ◽  
Radiation Induced ◽  
Extent Of Damage ◽  
Different Doses

The study was undertaken to evaluate gamma radiation-induced DNA damage in Aedes aegypti. The comet assay was employed to demonstrate the extent of DNA damage produced in adult male A. aegypti exposed to seven different doses of gamma radiation, ranging from 1 Gy to 50 Gy. DNA damage was measured as the percentage of comet tail DNA. A significant linear increase in DNA damage was observed in all samples; the extent of damage being proportional to the dose of gamma radiation the organism received, except in those treated with 1 Gy. The highest amount of DNA damage was noticed at 1 h postirradiation, which decreased gradually with time, that is, at 3, 6 and 12 h postirradiation. This may indicate repair of the damaged DNA and/or loss of heavily damaged cells as the postirradiation time increased. The comet assay serves as a sensitive and rapid technique to detect gamma radiation-induced DNA damage in A. aegypti. This could be used as a potential biomarker for environmental risk assessment.

Sign in / Sign up

Export Citation Format

Share Document