Expression of proteins that inhibit calcium oxalate crystallization in vitro in the urine of normal and stone-forming individuals

2001 ◽  
Vol 37 (1) ◽  
pp. 104-112 ◽  
Author(s):  
Ryan C. Hedgepeth ◽  
Lizhu Yang ◽  
Martin I. Resnick ◽  
Susan Ruth Marengo
Keyword(s):  
Calcium Oxalate ◽  
Calcium Oxalate Crystallization

scholarly journals In vitro effects on calcium oxalate crystallization kinetics and crystal morphology of an aqueous extract from Ceterach officinarum: Analysis of a potential antilithiatic mechanism

PLoS ONE ◽  
2019 ◽  
Vol 14 (6) ◽  
pp. e0218734 ◽  
Author(s):  
Roberta De Bellis ◽  
Maria Piera Piacentini ◽  
Maria Assunta Meli ◽  
Michele Mattioli ◽  
Michele Menotta ◽  
...  
Keyword(s):  
Calcium Oxalate ◽  
Crystallization Kinetics ◽  
Aqueous Extract ◽  
Crystal Morphology ◽  
Calcium Oxalate Crystallization ◽  
In Vitro Effects

Evaluation of Antiurolithiatic activity of Polyherbal formulation (Lithout Tablets) by In-vitro inhibition of Calcium Oxalate Crystallization

2019 ◽  
Vol 12 (11) ◽  
pp. 5477
Author(s):  
Pradnya N. Jagtap ◽  
Bhagyashri R. Vyapari ◽  
Yashashri H. Nimbalkar ◽  
Sitaram V. Kale ◽  
Ganesh B. Nigade
Keyword(s):  
Calcium Oxalate ◽  
Calcium Oxalate Crystallization ◽  
Polyherbal Formulation ◽  
In Vitro Inhibition

In vitro effect of lemon and orange juices on calcium oxalate crystallization

2007 ◽  
Vol 40 (3) ◽  
pp. 589-594 ◽  
Author(s):  
Sevsen Kulaksızoğlu ◽  
Mustafa Sofikerim ◽  
Cemal Çevik
Keyword(s):  
Calcium Oxalate ◽  
Calcium Oxalate Crystallization ◽  
Vitro Effect

scholarly journals In Vitro Effect of Wheat Bran (Triticum aestivum) Extract on Calcium Oxalate Urolithiasis Crystallization

2011 ◽  
Vol 6 (10) ◽  
pp. 1934578X1100601
Author(s):  
Khaled Sekkoum ◽  
Abdelkrim Cheriti ◽  
Safia Taleb
Keyword(s):  
Triticum Aestivum ◽  
Calcium Oxalate ◽  
Wheat Bran ◽  
Triticum Aestivum L ◽  
Inhibitory Effect ◽  
Calcium Oxalate Crystallization ◽  
Inhibiting Effect ◽  
Polarized Microscopy ◽  
Vitro Effect

Urolithiasis can lead to the loss of renal function in some cases. In this study, we tested the inhibiting effect of wheat bran ( Triticum aestivum L) extract on calcium oxalate crystallization in a turbidimetric model, by FTIR spectroscopy, and polarized microscopy. The results show that this plant extract has a major inhibitory effect on calcium oxalate crystallization.

Tamm-Horsfall Mucoprotein Reduces Promotion of Calcium Oxalate Crystal Aggregation Induced by Urate in Human Urine In Vitro

1994 ◽  
Vol 87 (2) ◽  
pp. 137-142 ◽  
Author(s):  
Phulwinder K. Grover ◽  
Villis R. Marshall ◽  
Rosemary L. Ryall
Keyword(s):  
Calcium Oxalate ◽  
Stone Formation ◽  
Urine Samples ◽  
Urine Specimen ◽  
Calcium Oxalate Crystal ◽  
Physiological Concentration ◽  
Calcium Oxalate Crystallization ◽  
Crystal Aggregation

1. Increasing the concentration of dissolved urate promotes calcium oxalate crystallization in urine from which Tamm-Horsfall mucoprotein, an inhibitor of calcium oxalate crystal aggregation, has almost completely been removed. This study aimed to determine whether the effect of urate could be reduced or abolished by a physiological concentration of Tamm-Horsfall mucoprotein. This was approached in two ways. 2. The effect of Tamm-Horsfall mucoprotein on calcium oxalate crystallization induced by urate was tested in ultrafiltered (10 kDa) urine samples from 10 healthy men. Tamm-Horsfall mucoprotein (35 mg/l) was added to half of each specimen, the urate concentration was increased by the addition of sodium urate solution and crystallization was induced by a standard load of oxalate. The remainder of each urine specimen was used as a control; these specimens were treated with an identical amount of urate solution, but contained no Tamm-Horsfall mucoprotein. Tamm-Horsfall mucoprotein had no effect on the urinary metastable limit or on the deposition of calcium oxalate, but significantly reduced the size of the particles precipitated. 3. The effect of increasing the urate concentration in the presence of Tamm-Horsfall mucoprotein was tested. Tamm-Horsfall mucoprotein (35 mg/l) was added to 10 ultrafiltered urine samples as before, the samples were divided, and the concentration of urate was increased in half of each specimen. Compared with the control to which no urate was added, urate significantly reduced the amount of oxalate required to induce spontaneous calcium oxalate nucleation and increased the median volume and the particle size of the material deposited. 4. It was concluded that, in vivo, (a) hyperuricosuria would encourage the formation of calcium oxalate stones by promoting calcium oxalate crystallization, (b) Tamm-Horsfall mucoprotein would not lessen the effect of urate on calcium oxalate nucleation or bulk deposition but would reduce its effect on crystal aggregation; it could therefore reduce the likelihood of stone formation in patients with hyperuricosuria.

Calcium oxalate crystallization in untreated urine, centrifuged and filtered urine and ultrafiltered urine

2004 ◽  
Vol 42 (1) ◽  
Author(s):  
Angela Guerra ◽  
Tiziana Meschi ◽  
Franca Allegri ◽  
Tania Schianchi ◽  
Giuditta Adorni ◽  
...  
Keyword(s):  
Calcium Oxalate ◽  
Urine Samples ◽  
Normal Male ◽  
Calcium Oxalate Crystallization ◽  
Different Types

AbstractCentrifuged and filtered urine is often used to evaluate in vitro the crystallization processes of calcium oxalate (CaOx), but even such simple manipulations can alter the composition of the urine, as regards its protein and lipid concentrations. In urine samples taken from 17 normal male adults, we evaluated CaOx crystallization by simultaneously using three different types of urine: untreated (U), centrifuged at 2000 rpm (800

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