Lipoprotein lipase steady-state mRNA levels are lower in human omental versus subcutaneous abdominal adipose tissue

The activity of lipoprotein lipase (LPL) in adipose tissue is modulated by changes in the nutritional status. We have measured LPL activity, mass, and mRNA levels in rat adipose tissue during normal feeding cycles, during short- and long-term fasting, and during refeeding after fasting. LPL activity displayed a diurnal rhythm. The activity was highest during the night and early morning, decreased to a minimum during the early afternoon, and then increased again. These changes corresponded to the feeding pattern. The increases and/or decreases resulted from changes in LPL synthetic rate compounded by posttranslational mechanisms. During short-term fasting, LPL specific activity decreased to < 30% of control. The specific activity was restored within 4 h by refeeding. On longer fasting, LPL mRNA decreased. This became significant from 36 h. On refeeding, it took 12 h to restore the mRNA levels, whereas tissue LPL activity and mass could not be fully restored by 36 h of refeeding. These data show that LPL activity during short-term fasting is regulated posttranscriptionally, which allows for quick upregulation after refeeding. On longer fasting, other mechanisms affecting LPL transcription and synthesis come into play, and upregulation after refeeding is slowed down.

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Subcutaneous Abdominal Adipose Tissue Lipolysis During Exercise Determined by Arteriovenous Measurements in Older Women

Journal of the American Geriatrics Society ◽

10.1046/j.1532-5415.2002.50059.x ◽

2002 ◽

Vol 50 (2) ◽

pp. 275-281 ◽

Cited By ~ 8

Author(s):

Kai Henrik Wiborg Lange ◽

Jeanne Lorentsen ◽

Fredrik Isaksson ◽

Lene Simonsen ◽

Anders Juul ◽

...

Keyword(s):

Adipose Tissue ◽

Older Women ◽

Abdominal Adipose Tissue ◽

Adipose Tissue Lipolysis ◽

Subcutaneous Abdominal Adipose Tissue

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Prediction of intra-abdominal and subcutaneous abdominal adipose tissue in healthy pre-pubertal children

International Journal of Obesity ◽

10.1038/sj.ijo.0800624 ◽

1998 ◽

Vol 22 (6) ◽

pp. 549-558 ◽

Cited By ~ 101

Author(s):

MI Goran ◽

BA Gower ◽

M Treuth ◽

TR Nagy

Keyword(s):

Adipose Tissue ◽

Abdominal Adipose Tissue ◽

Subcutaneous Abdominal Adipose Tissue

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Lack of antilipolytic effect of lactate in subcutaneous abdominal adipose tissue during exercise

Journal of Applied Physiology ◽

10.1152/jappl.1999.86.6.1800 ◽

1999 ◽

Vol 86 (6) ◽

pp. 1800-1804 ◽

Cited By ~ 8

Author(s):

François Trudeau ◽

Sylvain Bernier ◽

Isabelle de Glisezinski ◽

François Crampes ◽

François Dulac ◽

...

Keyword(s):

Adipose Tissue ◽

Subcutaneous Tissue ◽

Maximal Oxygen Consumption ◽

Physiological Saline ◽

Tissue Blood Flow ◽

Glycerol Concentration ◽

Abdominal Adipose Tissue ◽

Glycerol Level ◽

Good Physical Condition ◽

Subcutaneous Abdominal Adipose Tissue

The purpose of our study was to evaluate the potential inhibition of adipose tissue mobilization by lactate. Eight male subjects (age, 26.25 ± 1.75 yr) in good physical condition (maximal oxygen uptake, 59.87 ± 2.77 ml ⋅ kg−1 ⋅ min−1; %body fat, 10.15 ± 0.89%) participated in this study. For each subject, two microdialysis probes were inserted into abdominal subcutaneous tissue. Lactate (16 mM) was perfused via one of the probes while physiological saline only was perfused via the other, both at a flow rate of 2.5 μl/min. In both probes, ethanol was also perfused for adipose tissue blood flow estimation. Dialysates were collected every 10 min during rest (30 min), exercise at 50% maximal oxygen consumption (120 min), and recovery (30 min) for the measurement of glycerol concentration. During exercise, glycerol increased significantly in both probes. However, no differences in glycerol level and ethanol extraction were observed between the lactate and control probes. These findings suggest that lactate does not impair subcutaneous abdominal adipose tissue mobilization during exercise.

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An in vivo study of the cortisol-cortisone shuttle in subcutaneous abdominal adipose tissue

Clinical Endocrinology ◽

10.1046/j.1365-2265.1999.00598.x ◽

1999 ◽

Vol 50 (1) ◽

pp. 63-68 ◽

Cited By ~ 49

Author(s):

J. R. Katz ◽

V. Mohamed-Ali ◽

P. J. Wood ◽

J. S. Yudkin ◽

S. W. Coppack

Keyword(s):

Adipose Tissue ◽

In Vivo Study ◽

Abdominal Adipose Tissue ◽

Subcutaneous Abdominal Adipose Tissue

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Effects of Hypo- and Hyperthyroidism on Noradrenergic Activity and Glycerol Concentrations in Human Subcutaneous Abdominal Adipose Tissue Assessed with Microdialysis

The Journal of Clinical Endocrinology & Metabolism ◽

10.1210/jc.2003-030576 ◽

2003 ◽

Vol 88 (12) ◽

pp. 5605-5608 ◽

Cited By ~ 32

Author(s):

Martin Haluzik ◽

Jara Nedvidkova ◽

Vladimir Bartak ◽

Ivana Dostalova ◽

Petr Vlcek ◽

...

Keyword(s):

Adipose Tissue ◽

Thyroid Hormones ◽

Relative Magnitude ◽

In Vivo Microdialysis ◽

Abdominal Adipose Tissue ◽

Subcutaneous Abdominal Adipose Tissue ◽

Hypothyroid Patients ◽

Hyperthyroid Patients ◽

Tissue Norepinephrine

Abstract Thyroid hormones play a major role in lipid metabolism. However, whether they directly affect lipolysis locally in the adipose tissue remains unknown. Therefore, we measured abdominal sc adipose tissue norepinephrine (NE), basal, and isoprenaline-stimulated lipolysis in 12 hypothyroid patients (HYPO), six hyperthyroid patients (HYPER), and 12 healthy controls by in vivo microdialysis. Adipose tissue NE was decreased in HYPO and increased in HYPER compared with controls (90.4 ± 2.9 and 458.0 ± 69.1 vs. 294.9 ± 19.5 pmol/liter, P < 0.01). Similarly, basal lipolysis, assessed by glycerol assay, was lower in HYPO and higher in HYPER than in controls (88.2 ± 9.9 and 566.0 ± 42.0 vs. 214.3 ± 5.1 μmol/liter P < 0.01). The relative magnitude of isoprenaline-induced glycerol increase was smaller in HYPO (39 ± 19.4%, P < 0.05 vs. basal) and higher in HYPER (277 ± 30.4%, P < 0.01) than in controls (117 ± 5.6%, P < 0.01). The corresponding changes in NE after isoprenaline stimulation were as follows: 120 ± 9.2% (P < 0.05), 503 ± 113% (P < 0.01), and 267 ± 17.2 (P < 0.01). In summary, by affecting local NE levels and adrenergic postreceptor signaling, thyroid hormones may influence the lipolysis rate in the abdominal sc adipose tissue.

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