Lack of antilipolytic effect of lactate in subcutaneous abdominal adipose tissue during exercise

1999 ◽  
Vol 86 (6) ◽  
pp. 1800-1804 ◽  
Author(s):  
François Trudeau ◽  
Sylvain Bernier ◽  
Isabelle de Glisezinski ◽  
François Crampes ◽  
François Dulac ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Subcutaneous Tissue ◽  
Maximal Oxygen Consumption ◽  
Physiological Saline ◽  
Tissue Blood Flow ◽  
Glycerol Concentration ◽  
Abdominal Adipose Tissue ◽  
Glycerol Level ◽  
Good Physical Condition ◽  
Subcutaneous Abdominal Adipose Tissue

The purpose of our study was to evaluate the potential inhibition of adipose tissue mobilization by lactate. Eight male subjects (age, 26.25 ± 1.75 yr) in good physical condition (maximal oxygen uptake, 59.87 ± 2.77 ml ⋅ kg−1 ⋅ min−1; %body fat, 10.15 ± 0.89%) participated in this study. For each subject, two microdialysis probes were inserted into abdominal subcutaneous tissue. Lactate (16 mM) was perfused via one of the probes while physiological saline only was perfused via the other, both at a flow rate of 2.5 μl/min. In both probes, ethanol was also perfused for adipose tissue blood flow estimation. Dialysates were collected every 10 min during rest (30 min), exercise at 50% maximal oxygen consumption (120 min), and recovery (30 min) for the measurement of glycerol concentration. During exercise, glycerol increased significantly in both probes. However, no differences in glycerol level and ethanol extraction were observed between the lactate and control probes. These findings suggest that lactate does not impair subcutaneous abdominal adipose tissue mobilization during exercise.

scholarly journals Metabolic characteristics of human subcutaneous abdominal adipose tissueafter overnight fast

2012 ◽  
Vol 302 (4) ◽  
pp. E468-E475 ◽  
Author(s):  
Keith N. Frayn ◽  
Sandy M. Humphreys
Keyword(s):  
Adipose Tissue ◽  
Fatty Acid ◽  
Glucose Uptake ◽  
Human Adipose Tissue ◽  
Tissue Blood Flow ◽  
Nonesterified Fatty Acids ◽  
Abdominal Adipose Tissue ◽  
Adipose Tissue Metabolism ◽  
Tissue Metabolism ◽  
Subcutaneous Abdominal Adipose Tissue

Subcutaneous abdominal adipose tissue is one of the largest fat depots and contributes the major proportion of circulating nonesterified fatty acids (NEFA). Little is known about aspects of human adipose tissue metabolism in vivo other than lipolysis. Here we collated data from 331 experiments in 255 healthy volunteers over a 23-year period, in which subcutaneous abdominal adipose tissue metabolism was studied by measurements of arterio-venous differences after an overnight fast. NEFA and glycerol were released in a ratio of 2.7:1, different ( P < 0.001) from the value of 3.0 that would indicate no fatty acid re-esterification. Fatty acid re-esterification was 10.2 ± 1.4%. Extraction of triacylglycerol (TG) (fractional extraction 5.7 ± 0.4%) indicated intravascular lipolysis by lipoprotein lipase, and this contributed 21 ± 3% of the glycerol released. Glucose uptake (fractional extraction 2.6 ± 0.3%) was partitioned around 20–25% for provision of glycerol 3-phosphate and 30% into lactate production. There was release of lactate and pyruvate, with extraction of the ketone bodies 3-hydroxybutyrate and acetoacetate, although these were small numerically compared with TG and glucose uptake. NEFA release (expressed per 100 g tissue) correlated inversely with measures of fat mass (e.g., with BMI, rs= −0.24, P < 0.001). We examined within-person variability. Systemic NEFA concentrations, NEFA release, fatty acid re-esterification, and adipose tissue blood flow were all more consistent within than between individuals. This picture of human adipose tissue metabolism in the fasted state should contribute to a greater understanding of adipose tissue physiology and pathophysiology.

Subcutaneous Abdominal Adipose Tissue Blood Flow: Variation within and between Subjects and Relationship to Obesity

1996 ◽  
Vol 91 (6) ◽  
pp. 679-683 ◽  
Author(s):  
Lucinda K. M. Summers ◽  
Jaswinder S. Samra ◽  
Sandy M. Humphreys ◽  
Richard J. Morris ◽  
Keith N. Frayn
Keyword(s):  
Body Mass Index ◽  
Blood Flow ◽  
Adipose Tissue ◽  
Body Mass ◽  
Insulin Concentration ◽  
Tissue Blood Flow ◽  
Abdominal Adipose Tissue ◽  
Insulin Resistant ◽  
Adipose Tissue Blood Flow ◽  
Subcutaneous Abdominal Adipose Tissue

1. We assessed the variation in subcutaneous abdominal adipose tissue blood flow within and between subjects and investigated whether it is correlated with body mass index. 2. We measured body mass index and subcutaneous abdominal adipose tissue blood flow in 38 fasting subjects on the same day and on different days and, in a subgroup of 16 subjects, after a mixed meal. 3. In 190 measurements in the fasted state, subcutaneous abdominal adipose tissue blood flow was significantly more variable between subjects than could be accounted for by the within-subject variation alone. Subcutaneous abdominal adipose tissue blood flow was also significantly more variable between days within subjects than could be accounted for by within-day variation alone. Fasting and post-prandial subcutaneous abdominal adipose tissue blood flow were negatively correlated with body mass index, as was the post-prandial rise in subcutaneous abdominal adipose tissue blood flow. Multiple regression analysis showed that fasting blood flow was not dependent on insulin concentration after allowing for body mass index. There was no correlation between post-prandial subcutaneous abdominal adipose tissue blood flow and insulin concentration. 4. Insulin does not appear to have a direct vasodilatory effect in subcutaneous adipose tissue. Obese subjects have lower fasting and post-prandial subcutaneous abdominal adipose tissue blood flow. This may be because of a blunted response to sympathetic stimulation, or it may be another aspect of the insulin-resistant state.

scholarly journals GIP-induced vasodilation in human adipose tissue involves capillary recruitment

2019 ◽  
Vol 8 (6) ◽  
pp. 806-813 ◽  
Author(s):  
Meena Asmar ◽  
Ali Asmar ◽  
Lene Simonsen ◽  
Flemming Dela ◽  
Jens Juul Holst ◽  
...  
Keyword(s):  
Blood Flow ◽  
Adipose Tissue ◽  
Blood Volume ◽  
Tissue Blood Flow ◽  
Contrast Enhanced Ultrasound ◽  
Lipid Uptake ◽  
Abdominal Adipose Tissue ◽  
Capillary Recruitment ◽  
Contrast Enhanced ◽  
Subcutaneous Abdominal Adipose Tissue

Glucose-dependent insulinotropic polypeptide (GIP) in combination with hyperinsulinemia increase blood flow and triglyceride clearance in subcutaneous abdominal adipose tissue in lean humans. The present experiments were performed to determine whether the increase involves capillary recruitment. Eight lean healthy volunteers were studied before and after 1 h infusion of GIP or saline during a hyperglycemic–hyperinsulinemic clamp, raising plasma glucose and insulin to postprandial levels. Subcutaneous abdominal adipose tissue blood flow (ATBF) was measured by the 133Xenon clearance technique, and microvascular blood volume was determined by contrast-enhanced ultrasound imaging. During infusion of saline and the clamp, both ATBF (2.7 ± 0.5 mL/min 100 g/tissue) and microvascular blood volume remained unchanged throughout the experiments. During GIP infusion and the clamp, ATBF increased ~fourfold to 11.4 ± 1.9 mL/min 100 g/tissue, P < 0.001. Likewise, the contrast-enhanced ultrasound signal intensity, a measure of the microvascular blood volume, increased significantly 1 h after infusion of GIP and the clamp (P = 0.003), but not in the control experiments. In conclusion, the increase in ATBF during GIP infusion involves recruitment of capillaries in healthy lean subjects, which probably increases the interaction of circulating lipoproteins with lipoprotein lipase, thus promoting adipose tissue lipid uptake.

Subcutaneous Abdominal Adipose Tissue Lipolysis During Exercise Determined by Arteriovenous Measurements in Older Women

2002 ◽  
Vol 50 (2) ◽  
pp. 275-281 ◽  
Author(s):  
Kai Henrik Wiborg Lange ◽  
Jeanne Lorentsen ◽  
Fredrik Isaksson ◽  
Lene Simonsen ◽  
Anders Juul ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Older Women ◽  
Abdominal Adipose Tissue ◽  
Adipose Tissue Lipolysis ◽  
Subcutaneous Abdominal Adipose Tissue

Effect of training on epinephrine-stimulated lipolysis determined by microdialysis in human adipose tissue

1995 ◽  
Vol 269 (6) ◽  
pp. E1059-E1066 ◽  
Author(s):  
B. Stallknecht ◽  
L. Simonsen ◽  
J. Bulow ◽  
J. Vinten ◽  
H. Galbo
Keyword(s):  
Blood Flow ◽  
Adipose Tissue ◽  
Subcutaneous Adipose Tissue ◽  
Subcutaneous Tissue ◽  
Physical Work ◽  
Tissue Blood Flow ◽  
Epinephrine Infusion ◽  
Arterial Blood ◽  
Adipose Tissue Blood Flow ◽  
Subcutaneous Adipose

Trained humans (Tr) have a higher fat oxidation during submaximal physical work than sedentary humans (Sed). To investigate whether this reflects a higher adipose tissue lipolytic sensitivity to catecholamines, we infused epinephrine (0.3 nmol.kg-1.min-1) for 65 min in six athletes and six sedentary young men. Glycerol was measured in arterial blood, and intercellular glycerol concentrations in abdominal subcutaneous adipose tissue were measured by microdialysis. Adipose tissue blood flow was measured by 133Xe-washout technique. From these measurements adipose tissue lipolysis was calculated. During epinephrine infusion intercellular glycerol concentrations were lower, but adipose tissue blood flow was higher in trained compared with sedentary subjects (P < 0.05). Glycerol output from subcutaneous tissue (Tr: 604 +/- 322 nmol.100 g-1.min-1; Sed: 689 +/- 203; mean +/- SD) as well as arterial glycerol concentrations (Tr: 129 +/- 36 microM; Sed: 119 +/- 56) did not differ between groups. It is concluded that in intact subcutaneous adipose tissue epinephrine-stimulated blood flow is enhanced, whereas lipolytic sensitivity to epinephrine is the same in trained compared with untrained subjects.

An in vivo study of the cortisol-cortisone shuttle in subcutaneous abdominal adipose tissue

1999 ◽  
Vol 50 (1) ◽  
pp. 63-68 ◽  
Author(s):  
J. R. Katz ◽  
V. Mohamed-Ali ◽  
P. J. Wood ◽  
J. S. Yudkin ◽  
S. W. Coppack
Keyword(s):  
Adipose Tissue ◽  
In Vivo Study ◽  
Abdominal Adipose Tissue ◽  
Subcutaneous Abdominal Adipose Tissue
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