Phytotoxic tetranorditerpenoids from the fungus Sclerotinia homoeocarpa, causal agent of dollar spot in turfgrass

Planta Medica ◽  
2008 ◽  
Vol 74 (09) ◽  
Author(s):  
HMT Herath ◽  
WHMW Herath ◽  
SO Duke ◽  
M Tomaso-Peterson ◽  
NPD Nanayakkara
Keyword(s):  
Causal Agent ◽  
Sclerotinia Homoeocarpa ◽  
Dollar Spot

A Satellite RNA of Ophiostoma novo-ulmi Mitovirus 3a in Hypovirulent Isolates of Sclerotinia homoeocarpa

2004 ◽  
Vol 94 (9) ◽  
pp. 917-923 ◽  
Author(s):  
F. Deng ◽  
G. J. Boland
Keyword(s):  
Northern Blotting ◽  
Biological Data ◽  
Causal Agent ◽  
Dutch Elm Disease ◽  
Nucleotide Sequence Analysis ◽  
Sclerotinia Homoeocarpa ◽  
Dollar Spot ◽  
Satellite Rna ◽  
Stem Loop ◽  
Significant Difference

Two genetically distinct double-stranded RNA (dsRNA) elements were identified in hypovirulent isolates of Sclerotinia homoeocarpa, the causal agent of dollar spot of turfgrass. The large dsRNA (L-dsRNA) was consistently present in all hypovirulent isolates, whereas the small dsRNA (S-dsRNA) was found only in some hypovirulent isolates. Virulence comparisons revealed that there was no significant difference between isolates containing one or both dsRNAs. Therefore, the L-dsRNA appears to be the genetic determinant of hypovirulence, while the S-dsRNA is not essential for hypovirulence in S. homoeocarpa. The L-dsRNA in hypovirulent isolate Sh12B of S. homoeocarpa was previously characterized as a fungal mitochondrial virus and designated Ophiostoma novo-ulmi mitovirus 3a-Sh12B (OnuMV3a-Sh12B) because it was conspecific with O. novo-ulmi mitovirus 3a-Ld from O. novo-ulmi, the causal agent of Dutch elm disease. In the present study, the nucleotide sequences of the S-dsRNAs (738 to 767 nucleotides) in hypovirulent isolates Sh12B, Sh279B, and Sh286B were determined. Nucleotide sequence analysis indicated that the S-dsRNA was not derived from the OnuMV3a dsRNA and it could not encode an RNA-dependent RNA polymerase. These results are consistent with biological data that the S-dsRNA was always associated with the L-dsRNA and was never found independently. Therefore, the S-dsRNA can be regarded as a satellite RNA of OnuMV3a in S. homoeocarpa. Northern blotting analysis indicated that nucleic acid extracts from isolate Sh12B of S. homoeocarpa contained more single (+) stranded RNA than dsRNA for this satellite RNA. The 5′- and 3′-terminal sequences of the positive strand of the S-dsRNA each could be folded into a stem-loop structure and the terminal 21 nucleotides were complementary to each other, potentially forming a panhandle structure.

scholarly journals Antibiosis and Antagonism of Sclerotinia homoeocarpa and Drechslera poae by Pseudomonas fluorescens Pf-5 In Vitro and In Planta

1997 ◽  
Vol 87 (6) ◽  
pp. 614-621 ◽  
Author(s):  
Fanny Rodriguez ◽  
William F. Pfender
Keyword(s):  
Pseudomonas Fluorescens ◽  
Antibiotic Production ◽  
Disease Incidence ◽  
Interactive Effect ◽  
Causal Agent ◽  
Sclerotinia Homoeocarpa ◽  
Deficient Mutant ◽  
Dollar Spot ◽  
Moist Chamber

Pseudomonas fluorescens strain Pf-5, which produces several antifun-gal metabolites, including the antibiotics pyoluteorin, pyrrolnitrin, and 2,4-diacetylphloroglucinol, was tested for its ability to inhibit Sclerotinia homoeocarpa (causal agent of dollar spot) and Drechslerapoae (causal agent of ‘melting-out’) in vitro and in turfgrass; Tn5 mutants with altered antibiotic production also were tested. Inhibition in vitro differed with the medium used, but both fungi generally were inhibited by Pf-5. In most cases, a mutant deficient in pyoluteorin but not pyrrolnitrin or 2,4-di-acetylphloroglucinol was as inhibitory as Pf-5, whereas a pyrrolnitrin-deficient mutant was less inhibitory than Pf-5 in most fungus/medium combinations. High-performance liquid chromatography analysis of culture extracts showed that bacterial genotype and nutrition have an interactive effect on antibiotic production, such that conditions causing an increase in one antibiotic may increase or decrease another. The purported deficiencies for the pyrrolnitrin- and pyoluteorin-deficient mutants were confirmed. In S. homoeocarpa-infested grass clippings incubated in a moist chamber, Pf-5 reduced mycelial growth, whereas the pyrrolnitrin-deficient mutant did not and the pyoluteorin-deficient mutant was intermediate. In greenhouse experiments, Pf-5 reduced dollar spot disease incidence in bentgrass and bluegrass when sprayed over inoculated turf. In grass clippings infested with D. poae and incubated in a moist chamber under favorable conditions for spore production, Pf-5 did not reduce significantly the number of spores produced compared with the non-treated control. However, Pf-5 reduced melting-out disease incidence and severity in bluegrass inoculated with spores of D. poae under greenhouse conditions.

scholarly journals Hypovirulence and Double-Stranded RNA in Sclerotinia homoeocarpa

1997 ◽  
Vol 87 (2) ◽  
pp. 147-153 ◽  
Author(s):  
Ting Zhou ◽  
Greg J. Boland
Keyword(s):  
Creeping Bentgrass ◽  
Potato Dextrose Agar ◽  
Causal Agent ◽  
Curative Treatment ◽  
Sclerotinia Homoeocarpa ◽  
Dollar Spot ◽  
Double Stranded Rna ◽  
Virulent Isolate ◽  
Novel Approach ◽  
Detached Leaves

One hundred and thirty-two isolates of Sclerotinia homoeocarpa, the causal agent of dollar spot of turfgrass, were evaluated for virulence on swards and detached leaves of creeping bentgrass and for the presence of double-stranded RNA (dsRNA). In at least four isolates, the hypovirulent phenotype was associated with the presence of specific segments of dsRNA. In addition, these hypovirulent isolates often grew slowly on potato dextrose agar (PDA), formed thin colonies with atypical colony margins, and failed to produce typical black stroma. The hypovirulent phenotype and dsRNA were transmitted from hypovirulent isolate Sh12B to virulent isolate Sh48B, and the converted isolate was hypovirulent and contained dsRNA. The hypovirulent phenotype and dsRNA also were transmitted to at least four other isolates of the pathogen, including the fungicide-resistant, dsRNA¯ isolate KY-7. Converted isolates of KY-7 developed the hypovirulent phenotype, grew on fungicide-amended medium, and contained dsRNA. Subcultures of hypovirulent isolate Sh12B that did not contain dsRNA were obtained through curative treatment using cycloheximide-containing medium and heat. Cured subcultures grew faster on PDA, had more typical colony morphologies, were more virulent on bentgrass leaves, and did not contain dsRNA. No cured subcultures were obtained from hypovirulent isolate Sh09B. Isolates regenerated from protoplasts of hypovirulent isolate Sh12B were not cured, remained hypovirulent, and contained dsRNA. Transmission of hypovirulence and dsRNA in S. homoeocarpa has potential as a novel approach to the management of dollar spot of turfgrass.

scholarly journals First Report of Dollar Spot of Sandbur Caused by Sclerotinia homoeocarpa in Oklahoma

Plant Disease ◽  
2014 ◽  
Vol 98 (8) ◽  
pp. 1160-1160
Author(s):  
F. Flores ◽  
N. R. Walker
Keyword(s):  
Warm Season ◽  
Its Region ◽  
Small Subunit ◽  
Type I ◽  
Academic Press ◽  
Sclerotinia Homoeocarpa ◽  
Dollar Spot ◽  
First Report ◽  
Small Subunit Rdna ◽  
Disturbed Soils

Sandbur (Cenchrus incertus Curtis) is a warm-season, annual, noxious, grassy weed native to southern North America. It is common in sandy, disturbed soils and can also be found in home lawns and sport fields where low turf density facilitates its establishment. In July 2013, after a period of frequent rainfall and heavy dew, symptoms of dollar spot-like lesions (1) were observed on sandbur plants growing in a mixed stand of turf-type and native warm-season grasses in Logan County, Oklahoma. Lesions, frequently associated with leaf sheaths, were tan and surrounded by a dark margin. Symptomatic leaves were surface sterilized and plated on potato dextrose agar amended with 10 ppm rifampicin, 250 ppm ampicillin, and 5 ppm fenpropathrin. After incubation, a fungus morphologically identical to Sclerotinia homoeocarpa Bennett was consistently isolated. The nuclear ribosomal internal transcribed spacer (ITS) region of two different isolates, SCL2 and SCL3, were amplified using primers ITS4 and ITS5 (2). The DNA products were sequenced and BLAST analyses were used to compare sequences with those in GenBank. The sequence for isolate SLC2 was 869 bp, contained a type I intron in the 18S small subunit rDNA, and was identical to accession EU123803. The ITS sequence for isolate SLC3 was 535 bp and identical to accession EU123802. Twenty-five-day-old seedlings of C. incertus were inoculated by placing 5-mm-diameter agar plugs, colonized by mycelia of each S. homoeocarpa isolate, onto two of the plants' leaves. Plugs were held in place with Parafilm. Two plants were inoculated with each isolate and sterile agar plugs were placed on two leaves of another seedling as control. Plants were incubated in a dew chamber at 20°C and a 12-h photoperiod. After 3 days of incubation, water-soaked lesions surrounded by a dark margin appeared on inoculated plants only. Fungi that were later identified as S. homoeocarpa isolates SLC2 and SLC3 by sequencing of the ITS region were re-isolated from symptomatic leaves, fulfilling Koch's postulates. To our knowledge, this is the first report of dollar spot on sandbur. References: (1) R. W. Smiley et al. Page 22 in: Compendium of Turfgrass Diseases. 3rd ed. The American Phytopathological Society, St. Paul, MN, 2005. (2) T. J. White et al. Page 315 in: PCR Protocols: A Guide to Methods and Applications. Academic Press, San Diego, CA, 1990.

scholarly journals The Association Between In Vitro Propiconazole Sensitivity and Field Efficacy of Five New England Sclerotinia homoeocarpa Populations

Plant Disease ◽  
2012 ◽  
Vol 96 (4) ◽  
pp. 552-561 ◽  
Author(s):  
James T. Popko ◽  
Chang-Ho Ok ◽  
Katie Campbell-Nelson ◽  
Geunhwa Jung
Keyword(s):  
New England ◽  
Field Resistance ◽  
Golf Club ◽  
Sclerotinia Homoeocarpa ◽  
Dollar Spot ◽  
Mycelium Growth ◽  
Turfgrass Disease ◽  
Country Club ◽  
Field Efficacy

Dollar spot (Sclerotinia homoeocarpa) is a major turfgrass disease requiring fungicide application to maintain acceptable conditions for golf. A 2-year field experiment was conducted to determine the association between field efficacy of propiconazole and in vitro fungicide sensitivity of isolates from five S. homoeocarpa populations. Four golf courses with prior propiconazole exposure (Hartford Golf Club, Hickory Ridge Country Club, Shuttle Meadow Country Club, and Wintonbury Hills Golf Club), and a baseline site with no prior propiconazole exposure (Joseph Troll Turf Research Facility) were chosen as field sites. Experimental plots at each site received the following treatments at 21-day intervals: untreated, propiconazole (0.44, 0.88, 1.32, and 1.76 kg a.i. ha–1), and chlorothalonil (8.18 kg a.i. ha–1). S. homoeocarpa isolates were sampled at three time points during 2009 and 2010: initial (directly before fungicide treatment), 7 days after treatment (DAT), and 21 days after the last treatment. Isolates sampled from dollar spot infection centers at 7 DAT (2009 and 2010) were considered to exhibit “practical field resistance”. In parallel, S. homoeocarpa isolates from each site were assayed for in vitro sensitivity to propiconazole by determining relative mycelium growth percentages (RMG%) on potato dextrose agar amended with propiconazole at a discriminatory concentration of 0.1 μg a.i. ml–1. S. homoeocarpa isolates from the four exposed populations displayed significantly higher RMG% values than the baseline population. In general, field efficacy at all propiconazole rates tested was lower at the four locations with prior propiconazole exposure when compared with the baseline population. Increased RMG% values on the propiconazole discriminatory concentration 0.1 μg a.i. ml–1 were associated with decreased relative control values for all propiconazole rates in 2009 and 2010. Results suggest RMG values above 50% at the propiconazole discriminatory concentration of 0.1 μg a.i. ml–1 may be a suitable threshold for detection of S. homoeocarpa isolates that cause practical DMI field resistance.

scholarly journals Residual Efficacy of Fungicides for Control of Dollar Spot on Creeping Bentgrass

Plant Disease ◽  
2006 ◽  
Vol 90 (5) ◽  
pp. 571-575 ◽  
Author(s):  
Richard Latin
Keyword(s):  
Creeping Bentgrass ◽  
Effective Concentration ◽  
Disease Suppression ◽  
Golf Course ◽  
Sclerotinia Homoeocarpa ◽  
Dollar Spot ◽  
Residual Efficacy ◽  
Bioassay Technique ◽  
Precipitous Decline ◽  
Field Plots

The duration of effective concentrations of fungicides for control of dollar spot on creeping bentgrass fairways was investigated using a bioassay technique. In each of three runs of the experiment, fungicide treatments were applied once to turf in replicated field plots; then, the plots were sampled periodically over 3 weeks by removing turf plugs from the field. The sampled plugs were placed in incubation containers and inoculated with sections from a 4-day-old colony of Sclerotinia homoeocarpa growing on potato dextrose agar. After a 96-h incubation period, the extent of pathogen growth on the turf plugs was measured. Results described a precipitous decline in effective concentration for all fungicide treatments beginning 7 to 10 days after application. The fungicides were only marginally effective at 14 days after application, and none provided any disease suppression at 21 days after application. The efficacy half-life (EHL) associated with four of the fungicides (chlorothalonil, iprodione, propiconazole, and thiophanate methyl) was estimated with two models. EHL estimates ranged from 6.1 to 15.2 days depending on the fungicide and the model. This research contributes to our knowledge of the duration of effective fungicide concentrations on creeping bentgrass and can provide insight for scheduling fungicide sprays for golf course fairways.

scholarly journals Genetic Diversity of Sclerotinia homoeocarpa Isolates from Turfgrasses from Various Regions in North America

Plant Disease ◽  
2004 ◽  
Vol 88 (11) ◽  
pp. 1269-1276 ◽  
Author(s):  
G. Viji ◽  
W. Uddin ◽  
N. R. O'Neill ◽  
S. Mischke ◽  
J. A. Saunders
Keyword(s):  
United States ◽  
Genetic Diversity ◽  
Creeping Bentgrass ◽  
The United States ◽  
Vegetative Compatibility ◽  
Major Group ◽  
Golf Courses ◽  
Sclerotinia Homoeocarpa ◽  
Dollar Spot ◽  
Geographical Regions

Sixty-seven isolates of Sclerotinia homoeocarpa, causing dollar spot disease in creeping bentgrass, annual bluegrass, Bermudagrass, and perennial ryegrass turf, collected from 23 golf courses in various geographical regions of the United States and Canada between 1972 and 2001, were characterized by vegetative compatibility, genetic diversity, and pathogenicity. Eleven vegetative compatibility groups (VCGs A to K) were identified among the isolates tested in this study, and five of them (VCGs G to K) were new. VCG B was the most predominant group, typifying 33 isolates (51%) tested. S. homoeocarpa isolates collected from golf courses in Pennsylvania belonged to seven VCGs (A, B, E, F, G, I, and K), whereas three groups were observed in those collected from New York (B, E, and G) and New Jersey (E, H, and I). Two isolates, one each from Pennsylvania and Canada, were incompatible when paired with the tester isolates in all possible combinations, and did not fall into any known VCG. An isolate collected from Canada was compatible with tester isolates from two VCGs (C and D). Genetic analyses using amplified fragment length polymorphism (AFLP) showed the presence of two genetically distinct groups, designated as major group and the minor group. The major group included 36 isolates collected from various golf courses in the United States and Canada. Two isolates collected from bermudagrass in Florida formed a separate cluster, the minor group. Isolates that belonged to the major group were further divided into two subgroups (1 and 2). Subgroup 1 consisted of all the isolates that belonged to VCGs A, E, G, H, and I. Three of the four isolates that belonged to VCG K also were clustered with isolates of subgroup 1. Subgroup 2 consisted of all the isolates from VCG B, and one each from VCGs F and K. Pathogenicity assays on Penncross creeping bentgrass showed significant differences (P = 0.05) in virulence among the isolates. Overall, a relationship between virulence and VCGs was observed, in which certain virulence groups corresponded to specific VCGs; however, such a relationship was not observed between virulence and AFLPs. Close similarity among isolates of S. homoeocarpa collected from different locations in the United States and Canada suggests that isolates of the same genotype could be involved in outbreaks of dollar spot epidemics at multiple locations.

scholarly journals Mowing Frequency and Plant Growth Regulator Effects on Dollar Spot Severity and on Duration of Dollar Spot Control by Fungicides

Plant Disease ◽  
2011 ◽  
Vol 95 (11) ◽  
pp. 1433-1442 ◽  
Author(s):  
Alexander I. Putman ◽  
John E. Kaminski
Keyword(s):  
Plant Growth ◽  
Creeping Bentgrass ◽  
Agrostis Stolonifera ◽  
Golf Course ◽  
Sclerotinia Homoeocarpa ◽  
Dollar Spot ◽  
Confounding Effect ◽  
Residual Efficacy ◽  
The University ◽  
University Of Connecticut

Management of dollar spot (incited by Sclerotinia homoeocarpa) on golf course fairways is increasingly challenging. The objectives of this study were to determine the influence of mowing frequency and plant growth regulators (PGRs) on dollar spot severity and on the residual efficacy of fungicides for control of dollar spot. Two 4-month-long studies were conducted on ‘Putter’ creeping bentgrass (Agrostis stolonifera) maintained as a fairway at the University of Connecticut. Treatments were arranged in a three-by-three-by-five factorial that assessed the influence of mowing frequency (2, 4, or 6 days week–1) and PGRs (paclobutrazol, trinexapac-ethyl, or none) on dollar spot control by five fungicide treatments (boscalid, chlorothalonil, iprodione, propiconazole, or none). Turf was mowed in the afternoon hours to minimize the confounding effect of mowing frequency on leaf wetness duration. Treatments were initiated in the late spring of 2007 and 2008, and each fungicide treatment was reapplied only when dollar spot exceeded a threshold of five infection centers plot–1. In the absence of fungicides, dollar spot severity was reduced by 63 to 90% in plots treated with paclobutrazol and by 13 to 55% in plots treated with trinexapac-ethyl. Dollar spot severity was 23 to 50% lower in plots mown 2 days week–1 compared with those mown 6 days week–1. In cases where a significant interaction was observed between mowing frequency and PGRs, dollar spot was reduced on most rating dates in plots treated with trinexapacethyl that were mown 2 days week–1 compared with those mown 6 days week–1. Survival analysis of days until threshold was met revealed that duration of control of fungicides in plots receiving paclobutrazol were 28 to 84% longer compared with plots not receiving PGR. Duration of control by fungicides was generally similar between plots treated with trinexapac-ethyl and no PGR. In general, mowing frequency did not influence duration of control. Results from this study indicate that paclobutrazol could be used to increase the treatment interval of fungicides and that mowing frequency in the absence of dew is likely to have little influence on fungicide residual efficacy. When used without fungicides, PGRs and less frequent mowing may reduce dollar spot in situations where fungicide use is limited.

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