In Vitro Metabolism of Glucose in the Isolated Fat Cells of Rats Inoculated with a Growth Hormone Secreting Tumor (MtT-W15)

1971 ◽  
Vol 3 (05) ◽  
pp. 326-331 ◽  
Author(s):  
H. Åkerblom ◽  
J. Martin ◽  
G. Garay
Keyword(s):  
Growth Hormone ◽  
In Vitro Metabolism ◽  
Fat Cells ◽  
Isolated Fat Cells

LIPOLYSIS IN CHICKEN ADIPOSE TISSUE IN VITRO

1969 ◽  
Vol 43 (2) ◽  
pp. 285-294 ◽  
Author(s):  
D. R. LANGSLOW ◽  
C. N. HALES
Keyword(s):  
Growth Hormone ◽  
Adipose Tissue ◽  
Sodium Succinate ◽  
Fat Cells ◽  
Propranolol Hydrochloride ◽  
Isolated Fat Cells ◽  
Adipose Tissue In Vitro ◽  
High Concentrations ◽  
Long Acting

SUMMARY The effects on lipolysis of various compounds have been studied in intact chicken adipose tissue and in isolated fat cells prepared from chicken adipose tissue. Glucagon stimulated lipolysis at concentrations down to 1 ng./ml. in intact pieces and 0·1 ng./ml. in isolated fat cells. The effect was enhanced by high concentrations of insulin. No anti-lipolytic effect of insulin was observed. Adrenaline, noradrenaline, porcine corticotrophin (ACTH) and long-acting ACTH were lipolytic but the effects were small and high concentrations were required. The adrenaline effect was blocked by propranolol hydrochloride. Dibutyryl 3′,5′-(cyclic)-AMP and theophylline stimulated lipolysis as did a combination of crude chicken growth hormone and hydrocortisone sodium succinate. It was concluded that the pattern of response of chicken adipose tissue was markedly different from that of the rat.

Reduced glucose incorporation to triglycerides following chronic exposure of human fat cells to growth hormone

1980 ◽  
Vol 95 (1) ◽  
pp. 129-133 ◽  
Author(s):  
Gudrun Nyberg ◽  
Stig Boström ◽  
Rolf Johansson ◽  
Ulf Smith
Keyword(s):  
Growth Hormone ◽  
Adipose Tissue ◽  
Glucose Metabolism ◽  
Human Growth ◽  
Human Adipose Tissue ◽  
Culture Technique ◽  
Fat Cells ◽  
Glucose Incorporation ◽  
Total Glucose

Abstract. Using the tissue culture technique we have recently demonstrated that long-term exposure of human adipose tissue to human growth hormone (GH) in vitro leads to an impairment in glucose incorporation into triglycerides. This effect was further studied in the present investigation. Biopsies of human adipose tissue which had been cultured for one week with or without GH were studied in subsequent short-term incubations where the conversion of glucose to CO2 and to total lipids was determined. The formation of CO2 was not changed by previous exposure of the biopsies to GH whereas the incorporation of glucose into triglycerides was reduced by about one third. Total glucose metabolism, as determined from the sum of the two pathways, was significantly reduced. The activities of three glycolytic enzymes were determined in biopsies of human adipose tissue which had been cultured with or without GH for one week. The activity of phosphofructokinase was reduced, while the hexokinase and the glucose-6-phosphate dehydrogenase activities were unchanged. The diminished activity of phosphofructokinase, the enzyme considered to be rate-limiting for glycolysis in human fat cells, may be responsible for the decreased rate of glucose metabolism found.

Effect of dihydroergotamine and growth hormone on lipolysis in isolated fat cells of the rat

1970 ◽  
Vol 48 (5) ◽  
pp. 324-326 ◽  
Author(s):  
N. Hotta ◽  
O. V. Sirek ◽  
A. Sirek
Keyword(s):  
Growth Hormone ◽  
Bovine Growth Hormone ◽  
Fat Cells ◽  
Isolated Fat Cells ◽  
Cell Preparation ◽  
Fat Cell ◽  
Lipolytic Effect ◽  
Mechanism Of Interaction

Dihydroergotamine (DHE) is lipolytic in the isolated fat cell preparation of Rodbell. Bovine growth hormone (GH) alone or in combination with dexamethasone causes lipolysis that is barely measurable. The combination of GH and DHE is equally ineffective. Incubation of fat cells with all three agents produces massive lipolysis that is by far greater than the sum of effects of individual components. The augmented lipolytic effect is suppressible with puromycin. The exact mechanism of interaction between GH, dexamethasone, and DHE has to be elucidated.

scholarly journals Lipid Peroxidation in Vitro by Isolated Fat Cells of Rats

1969 ◽  
Vol 244 (16) ◽  
pp. 4382-4386
Author(s):  
V R Lavis ◽  
A E Kitabchi ◽  
R H Williams
Keyword(s):  
Lipid Peroxidation ◽  
Fat Cells ◽  
Isolated Fat Cells

EFFECTS OF PITUITARY HORMONES AND CORTICOSTERONE ON LIPOLYSIS IN HYPOPHYSECTOMIZED RATS

1974 ◽  
Vol 77 (3) ◽  
pp. 502-508 ◽  
Author(s):  
Ekkehard Schillinger ◽  
Erich Gerhards
Keyword(s):  
Growth Hormone ◽  
Adrenal Gland ◽  
Pituitary Gland ◽  
Hormonal Regulation ◽  
Pituitary Hormones ◽  
Fat Cells ◽  
Isolated Fat Cells ◽  
Hypophysectomized Rats ◽  
Pituitary Homogenate

ABSTRACT Removal of the pituitary gland in rats reduces basal lipolysis and greatly diminishes the lipolytic response of isolated fat cells towards L-epinephrine, ACTH and dibutyryl-cyclic-3′5′-AMP (DB-cAMP). Subcutaneous application for 5 days of ACTH, LH, FSH, GH and TSH, single or in combination, does not appreciably influence the responsiveness of fat cells towards lipolytic stimulation. Administration of a hypophyscal homogenate restores the sensitivity of fat cells of hypophysectomized rats. A similar effect is obtained with a combination of growth hormone and corticosterone. In hypophysectomized-adrenalectomized animals only the combination of growth hormone and corticosterone, but not a pituitary homogenate, produces a normal responsiveness of fat cells towards lipolytic stimuli. It is concluded that both the pituitary and adrenal gland are intimately involved in the hormonal regulation of lipolysis in fat cells.

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