Growth hormone effects on in vitro metabolism of avian adipose and liver tissue

The effect of prolonged feeding (3–5 months) of pemmican on some aspects of the in vitro metabolism of liver tissue was investigated. The endogenous metabolism of liver slices and homogenates was significantly increased by pemmican, probably due to an increase in the amount of readily metabolizable substrate. Utilizing mitochondrial preparations, it was found that with all substrates studied, except glutamate, α-ketoglutarate and succinate, the respiratory rate was not affected by the previous diet in the absence of added cytochrome c and diphosphopyridine nucleotide. The three substrates mentioned were oxidized at significantly lower rates in the pemmican group. Upon the addition of cytochrome c and diphosphopyridine nucleotide the qo2 of glutamate, α-ketoglutarate, and succinate was returned to the normal value.

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Metabolic effects of the major component of bovine growth hormone

Biochemical Journal ◽

10.1042/bj1220633 ◽

1971 ◽

Vol 122 (5) ◽

pp. 633-640 ◽

Cited By ~ 5

Author(s):

N. I. Swislocki ◽

M. Sonenberg ◽

M. Kikutani

Keyword(s):

Growth Hormone ◽

Deae Cellulose ◽

Heterogeneous Material ◽

Parent Material ◽

Metabolic Effects ◽

Sedimentation Equilibrium ◽

Bovine Growth Hormone ◽

Hormone Effects

Bovine growth hormone, subjected to DEAE-cellulose chromatography, yielded one major and several minor components. The various chromatographic fractions of bovine growth hormone were compared with the parent material for their ability to promote hormone effects in vivo and in vitro. The major component of bovine growth hormone was homogeneous by acrylamide-gel electrophoresis, rechromatography and sedimentation equilibrium. Its amino acid composition was similar to that of the parent hormone. The major component possessed all the qualitative activities present in the original heterogeneous material, including promotion of acute hypoglycaemia and hypolipaemia. In studies in vitro in adipose-tissue segments the major component of the hormone increased entry of glucose and its oxidation to CO2, conversion of glucose into glyceride glycerol, release of glycerol and incorporation of histidine into adiposetissue protein. Other chromatographic fractions of bovine growth hormone were not homogeneous and possessed some but not all of the metabolic activities attributed to the hormone preparations or its major component. Thus, the metabolic effects obtained with bovine growth-hormone preparations in vivo and in vitro can be obtained with the major homogeneous component of the hormone. This observation precludes the possibility that the metabolic effects obtained with bovine growth-hormone preparations are due to the combined actions of a number of components found therein.

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In Vitro Metabolism of Glucose in the Isolated Fat Cells of Rats Inoculated with a Growth Hormone Secreting Tumor (MtT-W15)

Hormone and Metabolic Research ◽

10.1055/s-0028-1094136 ◽

1971 ◽

Vol 3 (05) ◽

pp. 326-331 ◽

Cited By ~ 3

Author(s):

H. Åkerblom ◽

J. Martin ◽

G. Garay

Keyword(s):

Growth Hormone ◽

In Vitro Metabolism ◽

Fat Cells ◽

Isolated Fat Cells

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Application of in vitro [35S]GTPγ-S autoradiography in studies of growth hormone effects on opioid receptors in the male rat brain

Brain Research Bulletin ◽

10.1016/j.brainresbull.2012.09.008 ◽

2013 ◽

Vol 90 ◽

pp. 100-106 ◽

Cited By ~ 6

Author(s):

Jenny Johansson ◽

Alfhild Grönbladh ◽

Fred Nyberg ◽

Mathias Hallberg

Keyword(s):

Growth Hormone ◽

Rat Brain ◽

Opioid Receptors ◽

Male Rat ◽

Hormone Effects

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In vitro metabolism of aflatoxin B1 by normal and tumorous liver tissue from Thailand

Carcinogenesis ◽

10.1093/carcin/14.12.2613 ◽

1993 ◽

Vol 14 (12) ◽

pp. 2613-2620 ◽

Cited By ~ 39

Author(s):

G. M. Kirby ◽

C. R. Wolf ◽

G. E. Neal ◽

D. J. Judah ◽

C. J. Henderson ◽

...

Keyword(s):

Aflatoxin B1 ◽

Liver Tissue ◽

In Vitro Metabolism

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In Vitro Metabolism of Glucose by Adipose Tissue from Rats Bearing a Growth Hormone Secreting Tumor (MtTW15)

Diabetes ◽

10.2337/diab.18.6.387 ◽

1969 ◽

Vol 18 (6) ◽

pp. 387-391 ◽

Cited By ~ 5

Author(s):

D. P. Chattopadhyay ◽

H. K. Akerblom ◽

J. M. Martin

Keyword(s):

Growth Hormone ◽

Adipose Tissue ◽

In Vitro Metabolism

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IN VITRO METABOLISM OF OESTROGENS IN LIVER TISSUE FROM DOMESTIC ANIMALS

Acta Endocrinologica ◽

10.1530/acta.0.0590682 ◽

1968 ◽

Vol 59 (4) ◽

pp. 682-688 ◽

Cited By ~ 1

Author(s):

Olav Lyngset ◽

Weiert Velle

Keyword(s):

Thin Layer ◽

Thin Layer Chromatography ◽

Liver Tissue ◽

Domestic Animals ◽

In Vitro Metabolism ◽

List Type ◽

Tissue Slices ◽

Fluorescence Reaction ◽

Layer Chromatography

ABSTRACT Tissue slices from horse, dog, pig, cow, sheep and goat livers were incubated aerobically with oestrone, 17β-oestradiol or 17α-oestradiol for 1 hour. Metabolites were characterized by thin layer chromatography, and colour and fluorescence reaction. In addition metabolites were quantitatively determined. Oestrone gave rise to: 17β-oestradiol (horse, pig, cow) and 17α-oestradiol (cow, sheep, goat). 17β-oestradiol gave rise to: oestrone (horse, pig, dog, cow, sheep, goat) and 17α-oestradiol (cow, sheep, goat). 17α-oestradiol gave rise to: oestrone (cow, sheep, goat).

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