Anticancer activity of peptide extracted from edible mushroom; Lentinus squarrosulus in human lung cancer cells

The inherent limitations, including serious side-effects and drug resistance, of current chemotherapies necessitate the search for alternative treatments especially for lung cancer. Herein, the anticancer activity of colicin N, bacteria-produced antibiotic peptide, was investigated in various human lung cancer cells. After 24 h of treatment, colicin N at 5–15 µM selectively caused cytotoxicity detected by MTT assay in human lung cancer H460, H292 and H23 cells with no noticeable cell death in human dermal papilla DPCs cells. Flow cytometry analysis of annexin V-FITC/propidium iodide indicated that colicin N primarily induced apoptosis in human lung cancer cells. The activation of extrinsic apoptosis evidenced with the reduction of c-FLIP and caspase-8, as well as the modulation of intrinsic apoptosis signaling proteins including Bax and Mcl-1 were observed via Western blot analysis in lung cancer cells cultured with colicin N (10–15 µM) for 12 h. Moreover, 5–15 µM of colicin N down-regulated the expression of activated Akt (p-Akt) and its upstream survival molecules, integrin β1 and αV in human lung cancer cells. Taken together, colicin N exhibits selective anticancer activity associated with suppression of integrin-modulated survival which potentiate the development of a novel therapy with high safety profile for treatment of human lung cancer.

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Potent anticancer activity of a new bismuth (III) complex against human lung cancer cells

Journal of Inorganic Biochemistry ◽

10.1016/j.jinorgbio.2016.12.006 ◽

2017 ◽

Vol 168 ◽

pp. 18-26 ◽

Cited By ~ 17

Author(s):

Ruizhuo Ouyang ◽

Yang Yang ◽

Xiao Tong ◽

Kai Feng ◽

Yaoqin Yang ◽

...

Keyword(s):

Lung Cancer ◽

Anticancer Activity ◽

Cancer Cells ◽

Human Lung ◽

Lung Cancer Cells ◽

Human Lung Cancer ◽

Human Lung Cancer Cells

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Peptides extracted from edible mushroom: Lentinus squarrosulus induces apoptosis in human lung cancer cells

Pharmaceutical Biology ◽

10.1080/13880209.2017.1325913 ◽

2017 ◽

Vol 55 (1) ◽

pp. 1792-1799 ◽

Cited By ~ 12

Author(s):

Arisara Prateep ◽

Somruethai Sumkhemthong ◽

Maneewan Suksomtip ◽

Pithi Chanvorachote ◽

Chatchai Chaotham

Keyword(s):

Lung Cancer ◽

Cancer Cells ◽

Human Lung ◽

Edible Mushroom ◽

Lung Cancer Cells ◽

Human Lung Cancer ◽

Human Lung Cancer Cells

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Enhanced oral bioavailability and anticancer activity of novel curcumin loaded mixed micelles in human lung cancer cells

Phytomedicine ◽

10.1016/j.phymed.2015.08.006 ◽

2015 ◽

Vol 22 (12) ◽

pp. 1103-1111 ◽

Cited By ~ 57

Author(s):

Sharvil Patil ◽

Bhavana Choudhary ◽

Atul Rathore ◽

Krishtey Roy ◽

Kakasaheb Mahadik

Keyword(s):

Lung Cancer ◽

Anticancer Activity ◽

Cancer Cells ◽

Oral Bioavailability ◽

Mixed Micelles ◽

Human Lung ◽

Lung Cancer Cells ◽

Human Lung Cancer ◽

Human Lung Cancer Cells

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Inhibitory effects of Actinidia Chinensis planch root extracts (acRoots) on human lung cancer cells through retinoic acid receptor beta

Molecular and Cellular Therapies ◽

10.26781/2052-8426-2017-02 ◽

2017 ◽

Vol 5 (1) ◽

Cited By ~ 1

Author(s):

Lingyan Wang ◽

Jiayun Hou ◽

Minghuan Zheng ◽

Lin Shi

Keyword(s):

Lung Cancer ◽

Cancer Cells ◽

Human Lung ◽

Retinoic Acid Receptor ◽

Lung Cancer Cells ◽

Human Lung Cancer ◽

Inhibitory Effects ◽

The Core ◽

Human Lung Cancer Cells ◽

Receptor Beta

Actinidia Chinensis Planch roots (acRoots) are used to treat many cancers, although the anti-tumor mechanism by which acRoots inhibit cancer cell growth remains unclear. The present study aims at investigating inhibitory effects of acRoots on human lung cancer cells and potential mechanisms. Our data demonstrate that the inhibitory effects of acRoots on lung cancer cells depend on genetic backgrounds and phenotypes of cells. We furthermore found the expression of metabolism-associated gene profiles varied between acRoots-hypersensitive (H460) or hyposensitive lung cancer cells (H1299) after screening lung cancer cells with different genetic backgrounds. We selected retinoic acid receptor beta (RARB) as the core target within metabolism-associated core gene networks and evaluated RARB changes and roles in cells treated with acRoots at different concentrations and timeframes. Hypersensitive cancer cells with the deletion of RARB expression did not response to the treatment with acRoots, while RARB deletion did not change effects of acRoots on hyposensitive cells. Thus, it seems that RARB as the core target within metabolism-associated networks plays important roles in the regulation of lung cancer cell sensitivity to acRoots.

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