Scanning Electron Microscopic Observation of Platelets in Hemostasis

SummaryTo observe the very early changes in platelets during hemostasis, exposed carotid arteries of 3 rabbits were punctured with a needle, immediately fixed by 0.5% glutaraldehyde solution and their inner surface examined with a scanning electron microscope (SEM). Many platelets adhered in each case to the inner surface of the artery around the wound and all of them, whether solitary or aggregated, showed a spheroidal form with several pseudopodia.Platelets in shed blood from the incised ear vein of 3 control and 3 heparinized rabbits were fixed by glutaraldehyde and observed by SEM. The percentage of aggregated platelets was 60.9% in the control and 84.1 % in the heparinized rabbits immediately after the incision. The difference was statistically significant (P < 0.01). Sixty seconds after the incision, the percentages of aggregated platelets in the samples from the control and heparinized rabbits were 78.8% and 62.2% respectively. The difference was significant (P < 0.01). In accordance with this phenomenon, the percentage of the number of flat platelets decreased and that of altered platelets increased in the control rabbits. In the heparinized rabbits, far fewer flat platelets and larger numbers of altered platelets were observed in the samples than in those of the control rabbits immediately after the bleeding. However, 15 or 30 seconds later, the platelets of the heparinized rabbits showed fewer changes than those of the control.

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Scanning electron microscopic observation of the cytoskeleton of cells on the inner surface of mouse calvaria.

Orthopedics & Traumatology ◽

10.5035/nishiseisai.36.1000 ◽

1988 ◽

Vol 36 (3) ◽

pp. 1000-1003

Author(s):

Yasuo Noguchi ◽

Yoichi Sugioka

Keyword(s):

Microscopic Observation ◽

Electron Microscopic Observation ◽

Electron Microscopic ◽

Scanning Electron Microscopic Observation ◽

Mouse Calvaria ◽

Inner Surface ◽

Scanning Electron Microscopic ◽

Scanning Electron

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A scanning electron microscopic study on dissolving process of cholesterol gallstones by chenodeoxycholic acid (CDCA)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100083485 ◽

1978 ◽

Vol 36 (2) ◽

pp. 522-523

Author(s):

T. Kanetaka ◽

M. Cho ◽

S. Kawamura ◽

T. Sado ◽

K. Hara

Keyword(s):

Electron Microscope ◽

Chenodeoxycholic Acid ◽

Outer Surface ◽

Electron Microscopic ◽

Cholesterol Gallstones ◽

The Core ◽

Scanning Electron Microscopic Study ◽

Scanning Electron Microscopic ◽

Acceleration Voltage ◽

Scanning Electron

The authors have investigated the dissolution process of human cholesterol gallstones using a scanning electron microscope(SEM). This study was carried out by comparing control gallstones incubated in beagle bile with gallstones obtained from patients who were treated with chenodeoxycholic acid(CDCA).The cholesterol gallstones for this study were obtained from 14 patients. Three control patients were treated without CDCA and eleven patients were treated with CDCA 300-600 mg/day for periods ranging from four to twenty five months. It was confirmed through chemical analysis that these gallstones contained more than 80% cholesterol in both the outer surface and the core.The specimen were obtained from the outer surface and the core of the gallstones. Each specimen was attached to alminum sheet and coated with carbon to 100Å thickness. The SEM observation was made by Hitachi S-550 with 20 kV acceleration voltage and with 60-20, 000X magnification.

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Human Stapes Crura: Normal Ultrastructure. Scanning Electron Microscopic Findings

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100116267 ◽

1975 ◽

Vol 33 ◽

pp. 528-529

Author(s):

M.D. Graham

Keyword(s):

Electron Microscope ◽

Surgical Treatment ◽

Scanning Electron Microscope ◽

Osmic Acid ◽

Human Cadaver ◽

Electron Microscopic ◽

Scanning Electron Microscopic ◽

Normal Human ◽

Microscopic Findings ◽

Scanning Electron

The recent development of the scanning electron microscope has added great impetus to the study of ultrastructural details of normal human ossicles. A thorough description of the ultrastructure of the human ossicles is required in order to determine changes associated with disease processes following medical or surgical treatment.Human stapes crura were obtained at the time of surgery for clinical otosclerosis and from human cadaver material. The specimens to be examined by the scanning electron microscope were fixed immediately in the operating room in a cold phosphate buffered 2% gluteraldehyde solution, washed with Ringers, post fixed in cold 1% osmic acid and dehydrated in graded alcohol. Specimens were transferred from alcohol to a series of increasing concentrations of ethyl alcohol and amyl acetate. The tissue was then critical point dried, secured to aluminum stubs and coated with gold, approximately 150A thick on a rotating stage in a vacuum evaporator. The specimens were then studied with the Kent-Cambridge S4-10 Scanning Electron Microscope at an accelerating voltage of 20KV.

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