Thrombus Formation Time in Hemophilia An In Vitro Study

SummaryAn in vitro study was performed with 14 hemophilia-A patients, using a simple wheel with an electrically driven motor to form thrombi.The findings are discussed in relationship to normal thrombi and a direct comparison was made in regard to rate of thrombus formation, thrombus length and morphological differences.A relationship between the above and clinical severity of the disease with some speculations on the possible role played by white and red cells on thrombus formation time, was also presented.

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Platelet aggregation and thrombus formation in diabetes mellitus: An in vitro study

Pathology ◽

10.3109/00313027009077146 ◽

1970 ◽

Vol 2 (4) ◽

pp. 307-316 ◽

Cited By ~ 10

Author(s):

Raelene L. Rathbone ◽

Neville G. Ardlie ◽

Colin J. Schwartz

Keyword(s):

Diabetes Mellitus ◽

Platelet Aggregation ◽

Thrombus Formation ◽

In Vitro Study ◽

Vitro Study

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In vitro study of matrix surface properties of porous granulated calcium phosphate ceramic materials made in Russia

Bulletin of Experimental Biology and Medicine ◽

10.1007/s10517-008-0128-0 ◽

2008 ◽

Vol 145 (4) ◽

pp. 499-503 ◽

Cited By ~ 4

Author(s):

V. I. Chissov ◽

I. K. Sviridova ◽

N. S. Sergeeva ◽

V. A. Kirsanova ◽

S. A. Achmedova ◽

...

Keyword(s):

Calcium Phosphate ◽

Surface Properties ◽

In Vitro Study ◽

Ceramic Materials ◽

Vitro Study ◽

Calcium Phosphate Ceramic ◽

Matrix Surface ◽

Made In

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Accuracy of digital complete-arch, multi-implant scans made in the edentulous jaw with gingival movement simulation: An in vitro study

Journal of Prosthetic Dentistry ◽

10.1016/j.prosdent.2020.12.037 ◽

2021 ◽

Author(s):

Nathalie Knechtle ◽

Daniel Wiedemeier ◽

Albert Mehl ◽

Andreas Ender

Keyword(s):

In Vitro Study ◽

Vitro Study ◽

Movement Simulation ◽

Made In ◽

Edentulous Jaw

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Prediction Of The Thrombus Formation In Coronary Arteries By The In Vitro Study

Atherosclerosis ◽

10.1016/j.atherosclerosis.2019.06.419 ◽

2019 ◽

Vol 287 ◽

pp. e141

Author(s):

P. Savvinova ◽

A. Kalinskaya ◽

H. Vasilieva ◽

A. Shpektor

Keyword(s):

Coronary Arteries ◽

Thrombus Formation ◽

In Vitro Study ◽

Vitro Study

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Acidosis induced by carbon dioxide insufflation decreases heparin potency: a risk factor for thrombus formation

Perfusion ◽

10.1177/0267659116677307 ◽

2016 ◽

Vol 32 (3) ◽

pp. 214-219 ◽

Cited By ~ 7

Author(s):

Karin A.M. Gorter ◽

Marco C. Stehouwer ◽

Bart P. Van Putte ◽

Eline A. Vlot ◽

Rolf T. Urbanus

Keyword(s):

Cardiopulmonary Bypass ◽

Unfractionated Heparin ◽

Thrombus Formation ◽

Open Heart Surgery ◽

In Vitro Study ◽

Vitro Study ◽

Platelet Poor Plasma ◽

Advantages And Disadvantages ◽

Thrombin Formation

Background: Since the introduction of CO2 insufflation during open heart surgery in our hospital, we incidentally observed thrombus formation in the dissected heart, in the pericardium and in the cardiotomy reservoir of the cardiopulmonary bypass system. Furthermore, we measured very high levels of pCO2, causing severe acidosis, in stagnant blood in the pericardium and cardiotomy reservoir. Objectives: In this in vitro study, we assessed the influence of acidosis and hypothermia on heparin potency and thrombin formation. Methods: We assessed heparin potency in function of pH (pH 5.0-7.4) and temperature (24-37°C) by comparing the activated partial thromboplastin time in platelet-poor plasma between samples with and without unfractionated heparin. We measured thrombin formation in platelet-poor plasma by means of fluorescent, calibrated, automated thrombography in function of pH (pH 5.0-7.4) and temperature (24-37°C). The parameters of interest were the endogenous thrombin potential and the peak amount of thrombin generation. Results: The major finding of this study is the significant decrease in the efficiency of unfractionated heparin in delaying thrombus formation at acidotic (pH 5.0-7.0) conditions (p=0.034-0.05). Furthermore, we found that thrombin formation is significantly increased at hypothermic (24-34°C) conditions (p=<0.001-0.01). Conclusions: Based on the results of our in-vitro study, we conclude that acidosis may lead to a decreased heparin potency. Acidosis, as induced by CO2 insufflation, may predispose patients to incidental thrombus formation in stagnant blood in the open thorax and in the cardiotomy reservoir. Hypothermia might further increase this risk. Therefore, we recommend reconsidering the potential advantages and disadvantages of using CO2 insufflation during cardiopulmonary bypass.

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